{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:57:56Z","timestamp":1792713476060,"version":"4.5.0"},"reference-count":76,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T00:00:00Z","timestamp":1580083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100002322","name":"coordena\u00e7\u00e3o de aperfei\u00e7oamento de pessoal de n\u00edvel superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:p>Idiopathic varicocele is closely associated with male infertility or subfertility. Sertoli cell is a very important regulator of spermatogenesis. We investigated the morphofunctional alterations in the Sertoli cell and its possible involvement in the establishment of testicular primary lesion in experimental left-sided varicocele, induced from peripuberty. Twenty-five male peripubertal rats (44 days postpartum [dpp]) were distributed into two groups: control (C) and varicocele (V). Experimental left varicocele was induced in rats through the partial ligature of the left renal vein. Euthanasia was performed at 100 dpp. Testicular histopathology and testosterone plasmatic level were evaluated. Transferrin and vimentin proteins were, respectively, used as immunomarkers of Sertoli cell function and structure. Significant reductions in vimentin and transferrin expressions were noticed in androgen-dependent stages (VII and VIII) of the seminiferous epithelium cycle in V rats; testosterone plasmatic level was also reduced. Bilateral testicular histopathological alterations were found in V rats, mainly massive germ cell desquamation. The histological damage and changes in protein expressions occurred bilaterally. The relevant impairment of the functional and structural characteristics of the Sertoli cell, together with the typical massive germ cell desquamation, indicates that Sertoli cell changes can primarily contribute to the significant testicular dysfunction associated with varicocele.<\/jats:p>","DOI":"10.1369\/0022155420902547","type":"journal-article","created":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T06:31:29Z","timestamp":1580106689000},"page":"185-198","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Sertoli Cell Alterations in Peripubertal Varicocelized Rats: Evidence of Primary Damage on Spermatogenesis"],"prefix":"10.1369","volume":"68","author":[{"given":"Andr\u00e9 da Costa","family":"Vaz","sequence":"first","affiliation":[{"name":"Laboratory of Developmental Biology, Department of Morphology and Genetics, Federal University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila Cicconi","family":"Paccola","sequence":"additional","affiliation":[{"name":"Laboratory of Developmental Biology, Department of Morphology and Genetics, Federal University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Talita Biude","family":"Mendes","sequence":"additional","affiliation":[{"name":"Laboratory of Developmental Biology, Department of Morphology and Genetics, Federal University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina Elizabeth Louren\u00e7o","family":"Cabral","sequence":"additional","affiliation":[{"name":"Laboratory of Developmental Biology, Department of Morphology and Genetics, Federal University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joana Nogu\u00e8res","family":"Simas","sequence":"additional","affiliation":[{"name":"Laboratory of Developmental Biology, Department of Morphology and Genetics, Federal University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Vendramini","sequence":"additional","affiliation":[{"name":"Laboratory of Developmental Biology, Department of Morphology and Genetics, Federal University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra Maria","family":"Miraglia","sequence":"additional","affiliation":[{"name":"Laboratory of Developmental Biology, Department of Morphology and Genetics, Federal University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,1,27]]},"reference":[{"key":"bibr1-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2012.198"},{"key":"bibr2-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(99)80385-9"},{"key":"bibr3-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1590\/S1677-55382006000400002"},{"key":"bibr4-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2012.197"},{"key":"bibr5-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1016\/j.ucl.2013.08.001"},{"key":"bibr6-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)60638-4"},{"key":"bibr7-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dei089"},{"issue":"1","key":"bibr8-0022155420902547","first-page":"302","volume":"36","author":"R\u00f3th E","year":"1997","journal-title":"Acta Chir Hung"},{"issue":"2","key":"bibr9-0022155420902547","first-page":"206","volume":"46","author":"Turner TT","year":"1996","journal-title":"Lab Anim Sci"},{"key":"bibr10-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.63.579"},{"key":"bibr11-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2013.12.006"},{"key":"bibr12-0022155420902547","doi-asserted-by":"publisher","DOI":"10.5603\/FM.2014.0050"},{"key":"bibr13-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1530\/REP-15-0135"},{"key":"bibr14-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)44719-9"},{"key":"bibr15-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2003.09.078"},{"key":"bibr16-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1530\/REP-14-0481"},{"key":"bibr17-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod31.1.195"},{"key":"bibr18-0022155420902547","first-page":"431","volume-title":"The Sertoli cell","author":"Griswold MD","year":"1993"},{"key":"bibr19-0022155420902547","first-page":"71","volume-title":"Infertility in the male","author":"Tripp BM","year":"1997"},{"key":"bibr20-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092200206"},{"key":"bibr21-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-09597-4_11"},{"key":"bibr22-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1007\/BF02463646"},{"key":"bibr23-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2005.06.007"},{"key":"bibr24-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod40.3.691"},{"issue":"20","key":"bibr25-0022155420902547","doi-asserted-by":"crossref","first-page":"9523","DOI":"10.1016\/S0021-9258(18)43419-9","volume":"255","author":"Skinner MK","year":"1980","journal-title":"J Biol Chem"},{"key":"bibr26-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12165"},{"key":"bibr27-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0190067"},{"key":"bibr28-0022155420902547","first-page":"201","volume-title":"The Sertoli cell","author":"Sylvester SR","year":"1993"},{"key":"bibr29-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-0496(199607)22:1<14::AID-PPUL3>3.0.CO;2-K"},{"key":"bibr30-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1111\/and.12011"},{"issue":"6","key":"bibr31-0022155420902547","volume":"14","author":"Zheng Y","year":"2008","journal-title":"Med Sci Monit"},{"key":"bibr32-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2003.08.010"},{"key":"bibr33-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.2007.00766.x"},{"key":"bibr34-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1542\/peds.100.1.112"},{"key":"bibr35-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/7.1.55"},{"key":"bibr36-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/7.1.78"},{"key":"bibr37-0022155420902547","doi-asserted-by":"publisher","DOI":"10.4103\/1008-682X.157398"},{"key":"bibr38-0022155420902547","unstructured":"Andersen ML, D\u2019Almeida V, Ko GM, Kawakami R, Martins PJF, de Magalh\u00e3es LE, Tufik S. [Ethical and practical principles of the use of experimental animals]. S\u00e3o Paulo, Brazil: UNIFESP; 2004. Portuguese."},{"issue":"6","key":"bibr39-0022155420902547","first-page":"570","volume":"57","author":"Cartner SC","year":"2007","journal-title":"Comp Med"},{"key":"bibr40-0022155420902547","unstructured":"Russell LD. Histological and histopathological evaluation of the testis. Clearwater, FL: Cache River Press; 1990, p. 286."},{"key":"bibr41-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1016\/S0940-2993(11)80260-5"},{"key":"bibr42-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)49278-0"},{"key":"bibr43-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2008.00898.x"},{"key":"bibr44-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.2008.951947"},{"key":"bibr45-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1042\/BC20040078"},{"key":"bibr46-0022155420902547","first-page":"131","volume":"16","author":"Mandarim-de-Lacerda CA","year":"1999","journal-title":"Braz J Morphol Sci"},{"key":"bibr47-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.30.1.23"},{"key":"bibr48-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1988.tb00954.x"},{"key":"bibr49-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-006-0168-3"},{"key":"bibr50-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-10-22"},{"key":"bibr51-0022155420902547","first-page":"509","volume-title":"The Sertoli cell","author":"Sar M","year":"1993"},{"issue":"5","key":"bibr52-0022155420902547","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1002\/j.1939-4640.1994.tb00468.x","volume":"15","author":"Sylvester SR","year":"1994","journal-title":"J Androl"},{"key":"bibr53-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)57018-6"},{"key":"bibr54-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)39502-2"},{"key":"bibr55-0022155420902547","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.891324"},{"key":"bibr56-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110252"},{"key":"bibr57-0022155420902547","first-page":"21","volume-title":"The Sertoli Cell","author":"Clermont Y","year":"1993"},{"key":"bibr58-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7698-0_11"},{"key":"bibr59-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(199707)248:3<391::AID-AR12>3.0.CO;2-K"},{"key":"bibr60-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-0735"},{"key":"bibr61-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12459"},{"key":"bibr62-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1006\/taap.1996.0055"},{"key":"bibr63-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1007\/BF00304701"},{"key":"bibr64-0022155420902547","doi-asserted-by":"publisher","DOI":"10.4161\/oxim.1.1.6843"},{"key":"bibr65-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1038\/aja.2010.94"},{"issue":"3","key":"bibr66-0022155420902547","volume":"20","author":"Tian L","year":"1991","journal-title":"Zhonghua Bing Li Xue Za Zhi"},{"key":"bibr67-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(99)00013-8"},{"key":"bibr68-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2004.00477.x"},{"key":"bibr69-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0091127"},{"key":"bibr70-0022155420902547","doi-asserted-by":"publisher","DOI":"10.3109\/01485019308988363"},{"issue":"9","key":"bibr71-0022155420902547","first-page":"883","volume":"57","author":"Nistal M","year":"2004","journal-title":"Arch Esp Urol"},{"key":"bibr72-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2265.2001.01302.x"},{"key":"bibr73-0022155420902547","first-page":"557","volume-title":"The Sertoli Cell","author":"Chakraborty J","year":"1993"},{"issue":"3","key":"bibr74-0022155420902547","first-page":"209","volume":"10","author":"Razi M","year":"2012","journal-title":"Iran J Reprod Med"},{"key":"bibr75-0022155420902547","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-017-2667-z"},{"issue":"6","key":"bibr76-0022155420902547","first-page":"5647","volume":"18","author":"Pan J","year":"2018","journal-title":"Mol Med Rep"}],"container-title":["Journal of Histochemistry &amp; Cytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/0022155420902547","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1369\/0022155420902547","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/0022155420902547","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:28:07Z","timestamp":1777501687000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1369\/0022155420902547"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,27]]},"references-count":76,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,3]]}},"alternative-id":["10.1369\/0022155420902547"],"URL":"https:\/\/doi.org\/10.1369\/0022155420902547","relation":{},"ISSN":["0022-1554","1551-5044"],"issn-type":[{"value":"0022-1554","type":"print"},{"value":"1551-5044","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,27]]}}}