{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T00:39:22Z","timestamp":1793407162259,"version":"4.5.0"},"reference-count":32,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2020,3,10]],"date-time":"2020-03-10T00:00:00Z","timestamp":1583798400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100002661","name":"fonds de la recherche scientifique - fnrs","doi-asserted-by":"publisher","award":["FC29559"],"award-info":[{"award-number":["FC29559"]}],"id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:p>Senescence-associated beta-galactosidase (SA-\u03b2-gal) activity assay is commonly used to evaluate the increased beta-galactosidase (\u03b2-gal) activity in senescent cells related to enhanced lysosomal activity. Although the optimal pH for \u03b2-gal is 4.0, this enzymatic activity has been most commonly investigated at a suboptimal pH by using histochemical reaction on fresh tissue material. In the current study, we optimized a SA-\u03b2-gal activity histochemistry protocol that can also be applied on cryopreserved hepatic tissue. This protocol was developed on livers obtained from control rats and after bile duct resection (BDR). A significant increase in \u03b2-gal liver activity was observed in BDR rats vs controls after 2 hr of staining at physiological pH 4.0 (6.98 \u00b1 1.19% of stained\/total area vs 0.38 \u00b1 0.22; p&lt;0.01) and after overnight staining at pH 5.8 (24.09 \u00b1 6.88 vs 0.12 \u00b1 0.08; p&lt;0.01). Although we noticed that \u03b2-gal activity staining decreased with cryopreservation time (from 4 to 12 months of storage at \u221280C; p&lt;0.05), the enhanced staining observed in BDR compared with controls remained detectable up to 12 months after cryopreservation ( p&lt;0.01). In conclusion, we provide an optimized protocol for SA-\u03b2-gal activity histochemical detection at physiological pH 4.0 on long-term cryopreserved liver tissue:<\/jats:p>","DOI":"10.1369\/0022155420913534","type":"journal-article","created":{"date-parts":[[2020,3,10]],"date-time":"2020-03-10T11:30:19Z","timestamp":1583839819000},"page":"269-278","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":35,"title":["An Optimized Protocol for Histochemical Detection of Senescence-associated Beta-galactosidase Activity in Cryopreserved Liver Tissue"],"prefix":"10.1369","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7452-8727","authenticated-orcid":false,"given":"Giulia","family":"Jannone","sequence":"first","affiliation":[{"name":"Laboratory of Pediatric Hepatology and Cell Therapy, Institut de Recherche Exp\u00e9rimentale et Clinique, Universit\u00e9 Catholique de Louvain, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milena","family":"Rozzi","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Hepatology and Cell Therapy, Institut de Recherche Exp\u00e9rimentale et Clinique, Universit\u00e9 Catholique de Louvain, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustapha","family":"Najimi","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Hepatology and Cell Therapy, Institut de Recherche Exp\u00e9rimentale et Clinique, Universit\u00e9 Catholique de Louvain, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anabelle","family":"Decottignies","sequence":"additional","affiliation":[{"name":"Genetic and Epigenetic Alterations of Genomes Group, de Duve Institute, Universit\u00e9 Catholique de Louvain, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etienne 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