{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T04:11:15Z","timestamp":1793419875678,"version":"4.5.0"},"reference-count":34,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T00:00:00Z","timestamp":1600041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"national institutes of health","doi-asserted-by":"publisher","award":["U54 NS065736"],"award-info":[{"award-number":["U54 NS065736"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:p>The detection of cutaneous phosphorylated alpha-synuclein (P-syn) in patients with Parkinson\u2019s disease (PD) has ranged from 30% to 100% across different studies. We hypothesize that part of the variability in P-syn detection is due to methodological differences using sections of different tissue thickness. Three skin biopsies were obtained from 29 individuals with PD and 21 controls. Tissues were cut into 10-, 20-, and 50-\u00b5m-thick sections and double-stained with protein gene product (PGP) 9.5 and P-syn. We quantified the deposition of P-syn with and without PGP 9.5 in sweat glands, pilomotor muscle, and blood vessels using confocal digital images of autonomic structures. Overall, the P-syn-positive rates with PGP 9.5 colocalization in subjects with PD were 100% using 50 \u00b5m sections, 90% using 20 \u00b5m sections, and 73% using 10 \u00b5m sections with 100% specificity. (No P-syn was detected within control subjects.) Without PGP 9.5, colocalization of the P-syn-positive rates was 100% for all samples, but specificity dropped below 70%. In this study, double-immunostained 50 \u00b5m skin biopsy tissue sections are superior to 20 and 10 \u00b5m tissue sections at detecting P-syn in subjects with PD. The increased sensitivity is likely secondary to a combination of greater volume of tissue analyzed and improved visualization of nerve fiber architecture.<\/jats:p>","DOI":"10.1369\/0022155420960250","type":"journal-article","created":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T03:10:54Z","timestamp":1600053054000},"page":"669-678","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":59,"title":["Phosphorylated Alpha-Synuclein Within Cutaneous Autonomic Nerves of Patients With Parkinson\u2019s Disease: The Implications of Sample Thickness on Results"],"prefix":"10.1369","volume":"68","author":[{"given":"Ningshan","family":"Wang","sequence":"first","affiliation":[{"name":"Center for Autonomic and Peripheral Nerve Disorders, Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, 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