{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:05:44Z","timestamp":1782173144562,"version":"3.54.5"},"reference-count":63,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T00:00:00Z","timestamp":1600387200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001691","name":"japan society for the promotion of science","doi-asserted-by":"publisher","award":["18H02964"],"award-info":[{"award-number":["18H02964"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"japan society for the promotion of science","doi-asserted-by":"publisher","award":["19K10040"],"award-info":[{"award-number":["19K10040"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:p>\n                    To demonstrate the ultrastructure of osteocytic osteolysis and clarify whether osteocytic osteolysis occurs independently of osteoclastic activities, we examined osteocytes and their lacunae in the femora and tibiae of 11-week-old male wild-type and Rankl\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice after injection of human parathyroid hormone (PTH) [1-34] (80 \u00b5g\/kg\/dose). Serum calcium concentration rose temporarily 1 hr after PTH administration in wild-type and Rankl\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice, when renal arteries and veins were ligated. After 6 hr, enlargement of osteocytic lacunae was evident in the cortical bones of wild-type and Rankl\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice, but not so in their metaphyses. Von Kossa staining and transmission electron microscopy showed broadly demineralized bone matrix peripheral to enlarged osteocytic lacunae, which contained fragmented collagen fibrils and islets of mineralized matrices. Nano-indentation by atomic force microscopy revealed the reduced elastic modulus of the PTH-treated osteocytic perilacunar matrix, despite the microscopic verification of mineralized matrix in that region. In addition,\n                    <jats:sup>44<\/jats:sup>\n                    Ca deposition was detected by isotope microscopy and calcein labeling in the eroded osteocytic lacunae of wild-type and Rankl\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice. Taken together, our findings suggest that osteocytes can erode the bone matrix around them and deposit minerals on their lacunar walls independently of osteoclastic activity, at least in the murine cortical bone.\u2003(J Histochem Cytochem 68: \u2013XXX, 2020)\n                  <\/jats:p>","DOI":"10.1369\/0022155420961375","type":"journal-article","created":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T03:53:48Z","timestamp":1600401228000},"page":"651-668","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Osteocytic Osteolysis in PTH-treated Wild-type and\n                    <i>Rankl<\/i>\n                    <sup>\u2212\/\u2212<\/sup>\n                    Mice Examined by Transmission Electron Microscopy, Atomic Force Microscopy, and Isotope Microscopy"],"prefix":"10.1369","volume":"68","author":[{"given":"Hiromi","family":"Hongo","sequence":"first","affiliation":[{"name":"Developmental Biology of Hard Tissue, Faculty of Dental Medicine, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoka","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Developmental Biology of Hard Tissue, Faculty of Dental Medicine, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masami","family":"Saito","sequence":"additional","affiliation":[{"name":"Bruker Japan K.K., Nano Surfaces & Metrology Division, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanako","family":"Tsuboi","sequence":"additional","affiliation":[{"name":"Dental Surgery, Haibara General Hospital, Makinohara, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomomaya","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Japan Ground Self Defense Force Camp Asaka, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muneteru","family":"Sasaki","sequence":"additional","affiliation":[{"name":"Department of Applied Prosthodontics, Medical and Dental Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miki","family":"Abe","sequence":"additional","affiliation":[{"name":"Developmental Biology of Hard Tissue, Faculty of Dental Medicine, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo","family":"Henrique Luiz de Freitas","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Federal University of Sergipe at Lagarto, Aracaju, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisayoshi","family":"Yurimoto","sequence":"additional","affiliation":[{"name":"Isotope Imaging Laboratory, Creative Research Institution, Hokkaido University, Sapporo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuyuki","family":"Udagawa","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Matsumoto Dental University, Shiojiri, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minqi","family":"Li","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Oral Biomedicine, School of Stomatology, Shandong University, Jinan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norio","family":"Amizuka","sequence":"additional","affiliation":[{"name":"Developmental Biology of Hard Tissue, Faculty of Dental Medicine, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,9,18]]},"reference":[{"key":"bibr1-0022155420961375","volume-title":"Endocrinology","volume":"112","author":"Podbesek R","year":"1983"},{"key":"bibr2-0022155420961375","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650070110"},{"key":"bibr3-0022155420961375","doi-asserted-by":"publisher","DOI":"10.1210\/en.2015-2028"},{"key":"bibr4-0022155420961375","doi-asserted-by":"publisher","DOI":"10.1007\/BF02279101"},{"key":"bibr5-0022155420961375","unstructured":"von Recklinghausen FV. 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