{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T03:57:40Z","timestamp":1791863860131,"version":"4.3.4"},"reference-count":22,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2021,7,14]],"date-time":"2021-07-14T00:00:00Z","timestamp":1626220800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010135","name":"finska l\u00e4kares\u00e4llskapet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010135","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010116","name":"medicinska underst\u00f6dsf\u00f6reningen liv och h\u00e4lsa","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010116","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010119","name":"stiftelsen dorothea olivia, karl walter och jarl walter perkl\u00e9ns minne","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010119","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009067","name":"k. albin johanssons stiftelse","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009067","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Turunmaa Duodecim Association"},{"DOI":"10.13039\/501100005609","name":"turun yliopisto","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005609","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:p>Cyclic AMP element modulator (CREM) is a transcription factor best known for its intricate involvement in spermatogenesis. The CREM gene encodes for multiple protein isoforms, which can enhance or repress transcription of target genes. Recent studies have identified fusion genes, with CREM as a partner gene in many neoplastic diseases. EWSR1-CREM fusion genes have been found in several mesenchymal tumors and in salivary gland carcinoma. These genes encode fusion proteins that include the C-terminal DNA-binding domain of CREM. We used a transcriptomic approach and immunohistochemistry to study the expression of CREM isoforms that include DNA-binding domains across human tissues. We found that CREM protein is widely expressed in almost all normal human tissues. A transcriptomic analysis of normal tissues and cancer showed that transcription of CREM can be altered in tumors, suggesting that also wild-type CREM may be involved in cancer biology. The wide expression of CREM protein in normal human tissues and cancer may limit the utility of immunohistochemistry for identification of tumors with CREM fusions:<\/jats:p>","DOI":"10.1369\/00221554211032008","type":"journal-article","created":{"date-parts":[[2021,7,15]],"date-time":"2021-07-15T02:36:15Z","timestamp":1626316575000},"page":"495-509","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":23,"title":["Expression of Transcription Factor\n                    <i>CREM<\/i>\n                    in Human Tissues"],"prefix":"10.1369","volume":"69","author":[{"given":"Heidi","family":"Kaprio","sequence":"first","affiliation":[{"name":"Department of Pathology, Institute of Biomedicine, Turku University Hospital and University of Turku, Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanina D.","family":"Heuser","sequence":"additional","affiliation":[{"name":"Department of Pathology, Institute of Biomedicine, Turku University Hospital and University of Turku, Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katri","family":"Orte","sequence":"additional","affiliation":[{"name":"Department of Pathology, Institute of Biomedicine, Turku University Hospital and University of Turku, Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikko","family":"Tukiainen","sequence":"additional","affiliation":[{"name":"Auria Biobank, Turku University Hospital and University of Turku, Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilmo","family":"Leivo","sequence":"additional","affiliation":[{"name":"Department of Pathology, Institute of Biomedicine, Turku University Hospital and University of Turku, Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Gardberg","sequence":"additional","affiliation":[{"name":"Department of Pathology, Institute of Biomedicine, Turku University Hospital and University of Turku, Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,7,14]]},"reference":[{"issue":"2","key":"bibr1-00221554211032008","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/S0959-437X(05)80274-6","volume":"2","author":"Brindle PK","year":"1992","journal-title":"Curr Opin Genet Dev"},{"issue":"1","key":"bibr2-00221554211032008","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S1357-2725(97)00093-9","volume":"30","author":"Sassone-Corsi P","year":"1998","journal-title":"Int J Biochem Cell Biol"},{"issue":"3","key":"bibr3-00221554211032008","doi-asserted-by":"crossref","DOI":"10.1002\/j.1460-2075.1993.tb05759.x","volume":"12","author":"Laoide BM","year":"1993","journal-title":"EMBO J"},{"key":"bibr4-00221554211032008","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031798"},{"issue":"2","key":"bibr5-00221554211032008","volume":"47","author":"Wu X","year":"2012","journal-title":"J Mol Neurosci"},{"issue":"5","key":"bibr6-00221554211032008","doi-asserted-by":"crossref","DOI":"10.1038\/mp.2017.80","volume":"23","author":"Miller ML","year":"2018","journal-title":"Mol Psychiatry"},{"issue":"1","key":"bibr7-00221554211032008","doi-asserted-by":"crossref","first-page":"e5679","DOI":"10.1097\/MD.0000000000005679","volume":"96","author":"Feng F","year":"2017","journal-title":"Medicine"},{"issue":"6","key":"bibr8-00221554211032008","volume":"34","author":"Wang Y","year":"2013","journal-title":"Tumour Biol"},{"issue":"9","key":"bibr9-00221554211032008","doi-asserted-by":"crossref","DOI":"10.1097\/PAS.0000000000001114","volume":"42","author":"Chapman E","year":"2018","journal-title":"Am J Surg Pathol"},{"issue":"2","key":"bibr10-00221554211032008","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1097\/PAP.0000000000000291","volume":"28","author":"Toper MH","year":"2021","journal-title":"Adv Anat Pathol"},{"issue":"12","key":"bibr11-00221554211032008","doi-asserted-by":"crossref","DOI":"10.1097\/PAS.0000000000001331","volume":"43","author":"Yoshida A","year":"2019","journal-title":"Am J Surg Pathol"},{"issue":"1","key":"bibr12-00221554211032008","doi-asserted-by":"crossref","DOI":"10.1186\/s13000-018-0752-6","volume":"13","author":"Segawa K","year":"2018","journal-title":"Diagn Pathol"},{"issue":"11","key":"bibr13-00221554211032008","doi-asserted-by":"crossref","DOI":"10.1038\/s41379-020-0646-5","volume":"33","author":"Argani P","year":"2020","journal-title":"Mod Pathol"},{"issue":"1","key":"bibr14-00221554211032008","doi-asserted-by":"crossref","first-page":"4128","DOI":"10.1007\/s12032-012-0412-8","volume":"30","author":"Cantile M","year":"2013","journal-title":"Med Oncol"},{"key":"bibr15-00221554211032008","doi-asserted-by":"publisher","DOI":"10.1186\/bcr2615"},{"issue":"11","key":"bibr16-00221554211032008","doi-asserted-by":"crossref","DOI":"10.1210\/endo.141.11.7829","volume":"141","author":"Sanborn BM.","year":"2000","journal-title":"Endocrinology"},{"issue":"4","key":"bibr17-00221554211032008","doi-asserted-by":"crossref","first-page":"e1003464","DOI":"10.1371\/journal.pgen.1003464","volume":"9","author":"Giacomini CP","year":"2013","journal-title":"PLoS Genet"},{"key":"bibr18-00221554211032008","volume":"12","author":"Park SJ","year":"2019","journal-title":"BMC Med Genomics"},{"issue":"12","key":"bibr19-00221554211032008","doi-asserted-by":"crossref","DOI":"10.1097\/PAS.0000000000001590","volume":"44","author":"Skalova A","year":"2020","journal-title":"Am J Surg Pathol"},{"key":"bibr20-00221554211032008","doi-asserted-by":"publisher","DOI":"10.1126\/science.1260419"},{"issue":"5","key":"bibr21-00221554211032008","volume":"21","author":"Chan JK","year":"2013","journal-title":"Int J Surg Pathol"},{"issue":"16","key":"bibr22-00221554211032008","volume":"61","author":"Yehia G","year":"2001","journal-title":"Cancer Res"}],"container-title":["Journal of Histochemistry &amp; Cytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/00221554211032008","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1369\/00221554211032008","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/00221554211032008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:28:20Z","timestamp":1777501700000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1369\/00221554211032008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,14]]},"references-count":22,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["10.1369\/00221554211032008"],"URL":"https:\/\/doi.org\/10.1369\/00221554211032008","relation":{},"ISSN":["0022-1554","1551-5044"],"issn-type":[{"value":"0022-1554","type":"print"},{"value":"1551-5044","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,14]]}}}