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Versican\u2019s potential as an immunomodulatory molecule makes it a promising therapeutic target for controlling host immune responses in the lungs. To establish changes to versican expression and accumulation during influenza A viral pneumonia, we document the temporal and spatial changes to versican mRNA and protein in concert with pulmonary inflammatory cell infiltration. These studies were performed in the lungs of wild-type C57BL6\/J mice on days 3, 6, 9, and 12 post-infection with influenza A virus using immunohistochemistry, in situ hybridization, and quantitative digital pathology. Using duplex in situ hybridization, we demonstrate that type I interferon signaling contributes significantly to versican expression in lung stromal cells. Our findings show that versican is a type I interferon\u2013stimulated gene in pulmonary fibroblasts and pericytes in the context of viral pneumonia. These data also provide a guide for future studies to determine the role of versican in the pulmonary immune response to influenza infection:<\/jats:p>","DOI":"10.1369\/00221554211054447","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T14:56:03Z","timestamp":1634741763000},"page":"691-709","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["Type I Interferon Signaling Increases Versican Expression and Synthesis in Lung Stromal Cells During Influenza Infection"],"prefix":"10.1369","volume":"69","author":[{"given":"Jourdan E.","family":"Brune","sequence":"first","affiliation":[{"name":"Center for Lung Biology, University of Washington, Seattle, Washington"},{"name":"Department of Comparative Medicine, University of Washington, Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary 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