{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T23:42:20Z","timestamp":1777851740225,"version":"3.51.4"},"reference-count":25,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2022,3,6]],"date-time":"2022-03-06T00:00:00Z","timestamp":1646524800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001691","name":"japan society for the promotion of science","doi-asserted-by":"publisher","award":["21H02702"],"award-info":[{"award-number":["21H02702"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:p>It is widely accepted that E-selectin, an inducible endothelial cell adhesion molecule, plays a critical role in the initial step of neutrophil recruitment to sites of acute inflammation. However, immunohistological analysis of E-selectin has been hampered by lack of E-selectin-specific monoclonal antibodies that can stain formalin-fixed, paraffin-embedded (FFPE) tissue sections. Here, we employed E-selectin\u2022IgM (a soluble form of E-selectin) as immunogen, and then, after negative selection with L-selectin\u2022IgM and P-selectin\u2022IgM and screening of FFPE sections of both COS-1 cells overexpressing E-selectin and acute appendicitis tissues, we successfully generated an E-selectin-specific monoclonal antibody capable of staining FFPE tissue sections. We used this antibody, designated U12-12, to perform quantitative immunohistological analysis of 390 colonic mucosal biopsy specimens representing ulcerative colitis. We found that the higher the histological disease activity, the greater the number of vessels expressing E-selectin, an observation consistent with previous analyses of frozen tissue sections. Furthermore, in active ulcerative colitis, E-selectin-expressing vessels contained neutrophils attached to endothelial cells, presumably in the process of extravasation, which eventually could cause epithelial damage. These results overall indicate that U12-12 is effective for E-selectin immunohistochemistry in archived FFPE samples representing various human diseases:<\/jats:p>","DOI":"10.1369\/00221554221085336","type":"journal-article","created":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T01:39:43Z","timestamp":1646617183000},"page":"299-310","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Vascular E-selectin Expression Detected in Formalin-fixed, Paraffin-embedded Sections With an E-selectin Monoclonal Antibody Correlates With Ulcerative Colitis Activity"],"prefix":"10.1369","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1158-7375","authenticated-orcid":false,"given":"Masataka","family":"Murahashi","sequence":"first","affiliation":[{"name":"Department of Tumor Pathology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akiya","family":"Kogami","sequence":"additional","affiliation":[{"name":"Department of Tumor Pathology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akifumi","family":"Muramoto","sequence":"additional","affiliation":[{"name":"Department of Tumor Pathology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitomi","family":"Hoshino","sequence":"additional","affiliation":[{"name":"Department of Tumor Pathology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4248-6929","authenticated-orcid":false,"given":"Tomoya O.","family":"Akama","sequence":"additional","affiliation":[{"name":"Faculty of Medical Sciences, University of Fukui, Eiheiji, Japan; Department of Pharmacology, Kansai Medical University, Hirakata, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8743-7031","authenticated-orcid":false,"given":"Junya","family":"Mitoma","sequence":"additional","affiliation":[{"name":"Department of Medical Life Sciences, School of Medical Life Sciences, Kyushu University of Health and Welfare, Nobeoka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7671-0648","authenticated-orcid":false,"given":"Takanori","family":"Goi","sequence":"additional","affiliation":[{"name":"First Department of Surgery"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsuhiro","family":"Hirayama","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Medicine, Shinshu University School of Medicine, Matsumoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6838-4931","authenticated-orcid":false,"given":"Takuma","family":"Okamura","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Medicine, Shinshu University School of Medicine, Matsumoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0091-8343","authenticated-orcid":false,"given":"Tadanobu","family":"Nagaya","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Medicine, Shinshu University School of Medicine, Matsumoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7607-0801","authenticated-orcid":false,"given":"Motohiro","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Tumor Pathology"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,3,6]]},"reference":[{"issue":"7","key":"bibr1-00221554221085336","doi-asserted-by":"crossref","DOI":"10.4049\/jimmunol.144.7.2558","volume":"144","author":"Wellicome SM","year":"1990","journal-title":"J Immunol"},{"key":"bibr2-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.24.9238"},{"key":"bibr3-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12318"},{"key":"bibr4-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199905)29:05<1551::AID-IMMU1551>3.0.CO;2-0"},{"key":"bibr5-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1126\/science.1701274"},{"issue":"2","key":"bibr6-00221554221085336","volume":"53","author":"Takada A","year":"1993","journal-title":"Cancer Res"},{"key":"bibr7-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0407503101"},{"key":"bibr8-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2007.01189.x"},{"key":"bibr9-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1111\/j.2047-2927.2014.00192.x"},{"key":"bibr10-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1016\/j.urolonc.2015.05.026"},{"key":"bibr11-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathol.2016.08.002"},{"key":"bibr12-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1016\/j.urolonc.2021.02.013"},{"key":"bibr13-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1369\/00221554211048551"},{"key":"bibr14-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(90)91400-M"},{"key":"bibr15-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(92)90015-Q"},{"issue":"12","key":"bibr16-00221554221085336","volume":"9","author":"Cellier C","year":"1997","journal-title":"Eur J Gastroenterol Hepatol"},{"key":"bibr17-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1136\/gut.43.1.40"},{"key":"bibr18-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.5.1673"},{"key":"bibr19-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)45372-5"},{"key":"bibr20-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80137-3"},{"key":"bibr21-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.20827"},{"key":"bibr22-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1038\/s41374-019-0267-0"},{"key":"bibr23-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2016.104"},{"key":"bibr24-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathol.2019.01.002"},{"key":"bibr25-00221554221085336","doi-asserted-by":"publisher","DOI":"10.1136\/gut.47.3.404"}],"container-title":["Journal of Histochemistry &amp; 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