{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T17:35:21Z","timestamp":1788716121405,"version":"build-2803163510"},"reference-count":24,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T00:00:00Z","timestamp":1664323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["21H02702"],"award-info":[{"award-number":["21H02702"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:p>Spi-B, a member of the E26 transformation-specific (ETS) family of transcription factors, plays an important role in B cell differentiation. Spi-B also functions in development of diffuse large B-cell lymphoma; thus, we hypothesized that it may participate in leukemogenesis of B-cell acute lymphoblastic leukemia (B-ALL). To test this hypothesis, we first generated an anti-Spi-B monoclonal antibody that recognized Spi-B on formalin-fixed, paraffin-embedded tissue sections. This antibody, designated S28-5, selectively stained B cell nuclei at the pre-plasma cell stage (including centrocytes and centroblasts in germinal centers) and nuclei of plasmacytoid dendritic cells, but not fully differentiated plasma cells, T cells, macrophages, or follicular dendritic cells. Employing S28-5, we then performed immunohistochemical staining of bone marrow aspiration biopsy specimens obtained from B-ALL patients ( n=62). Cases that showed stronger nuclear S28-5 signals than T-cell ALL were scored positive. In 26 (42%) of 62 specimens, leukemic cells showed nuclear Spi-B expression, and positivity was associated with patient age at diagnosis, and serum uric acid and creatinine levels. Moreover, Spi-B-positive patients demonstrated significantly shorter overall survival than did Spi-B-negative patients. These results suggest that Spi-B expression may serve as a prognostic indicator of B-ALL.<\/jats:p>","DOI":"10.1369\/00221554221130383","type":"journal-article","created":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T08:49:02Z","timestamp":1664354942000},"page":"683-694","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Expression and Clinical Significance of Spi-B in B-cell Acute Lymphoblastic Leukemia"],"prefix":"10.1369","volume":"70","author":[{"given":"Yuzuru","family":"Ariga","sequence":"first","affiliation":[{"name":"Department of Tumor Pathology, Faculty of Medical Sciences, University of Fukui, Eiheiji, Japan"},{"name":"Department of Pediatrics, Faculty of Medical Sciences, University of Fukui, Eiheiji, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shulin","family":"Low","sequence":"additional","affiliation":[{"name":"Department of Tumor Pathology, Faculty of Medical 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