{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T11:05:58Z","timestamp":1780657558910,"version":"3.54.1"},"reference-count":22,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T00:00:00Z","timestamp":1690156800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002794","name":"Cancerfonden","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003748","name":"Svenska S\u00e4llskapet f\u00f6r Medicinsk Forskning","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003748","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007232","name":"Radiumhemmets Forskningsfonder","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100007232","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100011727","name":"Stockholm l\u00e4ns landsting","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100011727","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:p>Telomerase reverse transcriptase ( TERT) gene aberrancies correlate to adverse prognosis in follicular thyroid carcinoma (FTC). As loss of 5-hydroxymethylcytosine (5hmC) has been associated with TERT promoter mutations in papillary thyroid carcinoma, this study sought to analyze the levels of 5hmC in a cohort of follicular thyroid tumors with available TERT data. A total of 29 tumors (26 FTCs, 2 follicular thyroid tumors of uncertain malignant potential, and 1 oncocytic thyroid carcinoma) with known TERT promoter mutational status and TERT gene expression were assessed for 5hmC immunoreactivity using two antibodies (clones RM236 and 4D9.) Slides were analyzed using a semiquantitative scoring system. Of the 10 tumor cases with aberrant TERT, only 1 scored negative with both antibodies (1\/10; 10%), whereas the remaining 9 cases (9\/10; 90%) exhibited some positivity for at least one antibody. Of the 19 TERT wild-type tumors, no case was scored negative using RM236, and 2 cases (2\/19; 11%) using 4D9. The differences between TERT promoter mutated and wild-type groups were non-significant. The sensitivity and specificity for 5hmC immunohistochemistry (IHC) to detect mutated cases were 10% and 100% (RM236) and 20% and 89% (4D9). Therefore, 5hmC IHC is not a sensitive marker for detecting TERT promoter mutations in follicular thyroid tumors.<\/jats:p>","DOI":"10.1369\/00221554231190437","type":"journal-article","created":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T08:31:16Z","timestamp":1690187476000},"page":"451-458","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["5hmC Immunohistochemistry: A Predictor of\n                    <i>TERT<\/i>\n                    Promoter Mutational Status in Follicular Thyroid Carcinoma?"],"prefix":"10.1369","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6544-5399","authenticated-orcid":false,"given":"Martin","family":"Hysek","sequence":"first","affiliation":[{"name":"Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden"},{"name":"Department of Pathology and Cancer Diagnostics, Karolinska University Hospital, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8884-2090","authenticated-orcid":false,"given":"Samuel L.","family":"Hellgren","sequence":"additional","affiliation":[{"name":"Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden"},{"name":"Department of Pathology and Cancer Diagnostics, Karolinska University Hospital, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Condello","sequence":"additional","affiliation":[{"name":"Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiyi","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catharina","family":"Larsson","sequence":"additional","affiliation":[{"name":"Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden"},{"name":"Department of Pathology and Cancer Diagnostics, Karolinska University Hospital, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Zedenius","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden"},{"name":"Department of Breast, Endocrine Tumors, and Sarcoma, Karolinska University Hospital, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5945-9081","authenticated-orcid":false,"given":"C. Christofer","family":"Juhlin","sequence":"additional","affiliation":[{"name":"Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden"},{"name":"Department of Pathology and Cancer Diagnostics, Karolinska University Hospital, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,7,24]]},"reference":[{"key":"bibr1-00221554231190437","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2018.12.003"},{"key":"bibr2-00221554231190437","doi-asserted-by":"publisher","DOI":"10.3390\/cancers11101443"},{"key":"bibr3-00221554231190437","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-2383"},{"key":"bibr4-00221554231190437","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2018.0695"},{"key":"bibr5-00221554231190437","doi-asserted-by":"publisher","DOI":"10.1530\/ERC-18-0050"},{"key":"bibr6-00221554231190437","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2013.446"},{"key":"bibr7-00221554231190437","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-3734"},{"key":"bibr8-00221554231190437","doi-asserted-by":"publisher","DOI":"10.1007\/s12022-022-09707-3"},{"key":"bibr9-00221554231190437","doi-asserted-by":"publisher","DOI":"10.1007\/s12022-018-9551-6"},{"key":"bibr10-00221554231190437","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2021-207631"},{"key":"bibr11-00221554231190437","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.07.033"},{"key":"bibr12-00221554231190437","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.27429"},{"key":"bibr13-00221554231190437","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2019.10531"},{"key":"bibr14-00221554231190437","doi-asserted-by":"publisher","DOI":"10.1007\/s12022-020-09652-z"},{"key":"bibr15-00221554231190437","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/2658727"},{"key":"bibr16-00221554231190437","doi-asserted-by":"publisher","DOI":"10.3390\/curroncol28050321"},{"issue":"5","key":"bibr17-00221554231190437","volume":"18","author":"Zhong J","year":"2019","journal-title":"Exp Ther Med"},{"key":"bibr18-00221554231190437","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2014-2624"},{"key":"bibr19-00221554231190437","doi-asserted-by":"publisher","DOI":"10.1016\/j.anndiagpath.2022.151903"},{"key":"bibr20-00221554231190437","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-021-02405-w"},{"key":"bibr21-00221554231190437","doi-asserted-by":"publisher","DOI":"10.1007\/s12022-019-09580-7"},{"key":"bibr22-00221554231190437","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2020.10.016"}],"container-title":["Journal of Histochemistry &amp; Cytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/00221554231190437","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1369\/00221554231190437","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/00221554231190437","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:25:51Z","timestamp":1777501551000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1369\/00221554231190437"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,24]]},"references-count":22,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["10.1369\/00221554231190437"],"URL":"https:\/\/doi.org\/10.1369\/00221554231190437","relation":{},"ISSN":["0022-1554","1551-5044"],"issn-type":[{"value":"0022-1554","type":"print"},{"value":"1551-5044","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,24]]}}}