{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T22:14:31Z","timestamp":1792016071585,"version":"4.4.0"},"reference-count":21,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T00:00:00Z","timestamp":1697155200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["DP2GM128198"],"award-info":[{"award-number":["DP2GM128198"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"name":"HHS | NIH | National Institute of Biomedical Imaging and Bioengineering","award":["2-P41-EB015871-31"],"award-info":[{"award-number":["2-P41-EB015871-31"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:p>In the clinical setting, routine identification of the main types of tissue amyloid deposits, light-chain amyloid (AL) and serum amyloid A (AA), is based on histochemical staining; rarer types of amyloid require mass spectrometry analysis. Raman spectroscopic imaging is an analytical tool, which can be used to chemically map, and thus characterize, the molecular composition of fluid and solid tissue. In this proof-of-concept study, we tested the feasibility of applying Raman spectroscopy combined with artificial intelligence to detect and characterize amyloid deposits in unstained frozen tissue sections from kidney biopsies with pathologic diagnosis of AL and AA amyloidosis and control biopsies with no amyloidosis (NA). Raman hyperspectral images, mapped in a 2D grid-like fashion over the tissue sections, were obtained. Three machine learning\u2013assisted analysis models of the hyperspectral images could accurately distinguish AL (types \u03bb and \u03ba), AA, and NA 93\u2013100% of the time. Although very preliminary, these findings illustrate the potential of Raman spectroscopy as a technique to identify, and possibly, subtype renal amyloidosis.<\/jats:p>","DOI":"10.1369\/00221554231206858","type":"journal-article","created":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T00:42:36Z","timestamp":1697244156000},"page":"643-652","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Hyperspectral Raman Imaging for Automated Recognition of Human Renal Amyloid"],"prefix":"10.1369","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5689-1445","authenticated-orcid":false,"given":"Jeong Hee","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering, Johns Hopkins University, Baltimore, Maryland, Johns Hopkins University School of Medicine, Baltimore, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1911-8258","authenticated-orcid":false,"given":"Chi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, Johns Hopkins University, Baltimore, Maryland, Johns Hopkins University School of Medicine, Baltimore, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8409-0714","authenticated-orcid":false,"given":"C. 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Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9092-3313","authenticated-orcid":false,"given":"Serena M.","family":"Bagnasco","sequence":"additional","affiliation":[{"name":"Clinical Renal Biopsy Service, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,10,13]]},"reference":[{"key":"bibr1-00221554231206858","doi-asserted-by":"publisher","DOI":"10.1007\/s40620-017-0426-6"},{"key":"bibr2-00221554231206858","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2019-206269"},{"key":"bibr3-00221554231206858","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.10491012"},{"key":"bibr4-00221554231206858","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2012.108"},{"key":"bibr5-00221554231206858","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.7b00413"},{"key":"bibr6-00221554231206858","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-16002-3"},{"key":"bibr7-00221554231206858","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2021.113115"},{"key":"bibr8-00221554231206858","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-190675"},{"key":"bibr9-00221554231206858","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1003281"},{"key":"bibr10-00221554231206858","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-021-00620-3"},{"key":"bibr11-00221554231206858","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.812388"},{"key":"bibr12-00221554231206858","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-161238"},{"key":"bibr13-00221554231206858","doi-asserted-by":"publisher","DOI":"10.1039\/C8CC03217C"},{"key":"bibr14-00221554231206858","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112863"},{"key":"bibr15-00221554231206858","doi-asserted-by":"publisher","DOI":"10.1039\/b821856k"},{"key":"bibr16-00221554231206858","doi-asserted-by":"publisher","DOI":"10.1038\/srep29096"},{"key":"bibr17-00221554231206858","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1002"},{"key":"bibr18-00221554231206858","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12898-9"},{"key":"bibr19-00221554231206858","doi-asserted-by":"publisher","DOI":"10.1016\/j.ekir.2018.03.010"},{"key":"bibr20-00221554231206858","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-018-9770-9"},{"key":"bibr21-00221554231206858","doi-asserted-by":"publisher","DOI":"10.1111\/jgh.14738"}],"container-title":["Journal of Histochemistry &amp; Cytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/00221554231206858","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1369\/00221554231206858","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/00221554231206858","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:25:46Z","timestamp":1777501546000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1369\/00221554231206858"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,13]]},"references-count":21,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2023,11]]}},"alternative-id":["10.1369\/00221554231206858"],"URL":"https:\/\/doi.org\/10.1369\/00221554231206858","relation":{},"ISSN":["0022-1554","1551-5044"],"issn-type":[{"value":"0022-1554","type":"print"},{"value":"1551-5044","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,13]]}}}