{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T04:38:46Z","timestamp":1781671126679,"version":"3.54.5"},"reference-count":23,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T00:00:00Z","timestamp":1704672000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"National Institute on Drug Abuse","award":["R01 DA030386"],"award-info":[{"award-number":["R01 DA030386"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:p>Human intestinal spirochetosis (HIS) is a colorectal bacterial infection caused by the Brachyspira species. Griffonia simplicifolia-II (GS-II) is a lectin specific to terminal \u03b1\/\u03b2GlcNAc residues. Here, we investigated terminal \u03b2GlcNAc residues in the context of HIS infection using GS-II-horseradish peroxidase staining and HIK1083 immunostaining specific to terminal \u03b1GlcNAc residues. Fourteen of 15 HIS cases were GS-II-positive on the bacterial body. No cases showed HIK1083 positivity. The percentage of bacterial bodies staining positively for GS-II based on comparison with anti- Treponema immunostaining was \u226430% in seven cases, 30\u201370% in two, and &gt;70% in six. Of 15 HIS cases analyzed, none were comorbid with tubular adenomas, and three were comorbid with sessile serrated lesions (SSLs). To determine the species of spirochete infected, the B. aalborgi-specific or B. pilosicoli-specific NADPH oxidase genes were amplified by PCR. After direct sequencing of the PCR products, all nine cases in which PCR products were observed were found to be infected with B. aalborgi alone. These results indicate that the HIS bacterial body, especially of B. aalborgi, is characterized by terminal \u03b2GlcNAc and also indicate that terminal \u03b2GlcNAc on the HIS bacterial body is associated with HIS preference for SSLs:<\/jats:p>","DOI":"10.1369\/00221554231222963","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T05:30:32Z","timestamp":1704691832000},"page":"71-78","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification of Terminal \u03b2GlcNAc on\n                    <i>Brachyspira<\/i>\n                    Species in Human Intestinal Spirochetosis"],"prefix":"10.1369","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9045-0835","authenticated-orcid":false,"given":"Hisanori","family":"Matoba","sequence":"first","affiliation":[{"name":"Department of Infection and Host Defense (HM); Department of Molecular Pathology (HM, CF, JN); Center for Medical Education and Clinical Training (CF), Shinshu University School of Medicine, Matsumoto, Japan; Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan (MI); Department of Biotechnology, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Matsumoto, Japan (CF); and Department of Pathology, North Alps Medical..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2135-2219","authenticated-orcid":false,"given":"Mai","family":"Iwaya","sequence":"additional","affiliation":[{"name":"Department of Infection and Host Defense (HM); Department of Molecular Pathology (HM, CF, JN); Center for Medical Education and Clinical Training (CF), Shinshu University School of Medicine, Matsumoto, Japan; Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan (MI); Department of Biotechnology, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Matsumoto, Japan (CF); and Department of Pathology, North Alps Medical..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8735-5274","authenticated-orcid":false,"given":"Chifumi","family":"Fujii","sequence":"additional","affiliation":[{"name":"Department of Infection and Host Defense (HM); Department of Molecular Pathology (HM, CF, JN); Center for Medical Education and Clinical Training (CF), Shinshu University School of Medicine, Matsumoto, Japan; Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan (MI); Department of Biotechnology, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Matsumoto, Japan (CF); and Department of Pathology, North Alps Medical..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6773-2802","authenticated-orcid":false,"given":"Jun","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Department of Infection and Host Defense (HM); Department of Molecular Pathology (HM, CF, JN); Center for Medical Education and Clinical Training (CF), Shinshu University School of Medicine, Matsumoto, Japan; Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan (MI); Department of Biotechnology, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, Matsumoto, Japan (CF); and Department of Pathology, North Alps Medical..."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,1,8]]},"reference":[{"key":"bibr1-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/570526"},{"key":"bibr2-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-020-02785-w"},{"key":"bibr3-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2006.04606.x"},{"key":"bibr4-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2014.03.007"},{"key":"bibr5-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2016.07.032"},{"key":"bibr6-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(76)90444-6"},{"key":"bibr7-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1111\/pin.13321"},{"key":"bibr8-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2010.212704"},{"key":"bibr9-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800987"},{"key":"bibr10-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2015.08.011"},{"key":"bibr11-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2011.12.004"},{"key":"bibr12-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.2000.01020-3.x"},{"key":"bibr13-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1309\/G7U6BD85W4G3WJ0J"},{"key":"bibr14-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1136\/gut.12.2.126"},{"key":"bibr15-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1995.tb01530.x"},{"key":"bibr16-00221554231222963","volume":"14","author":"Lilley C","year":"2022","journal-title":"Cureus"},{"key":"bibr17-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.1.347-350.2001"},{"key":"bibr18-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.11.4111-4118.2001"},{"key":"bibr19-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.6.2093-2098.1999"},{"key":"bibr20-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0052281"},{"key":"bibr21-00221554231222963","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-68.7.1525"},{"key":"bibr22-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00087-17"},{"key":"bibr23-00221554231222963","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00680-11"}],"container-title":["Journal of Histochemistry &amp; 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