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The phenotype exhibited by mice lacking TRPA1 suggests its potential as a target for pharmacological intervention. Antibody-based detection for distribution analysis is a standard technique. In the case of TRPA1, however, there is no antibody with a plausible validation in knockout animals or functional studies, but many that have failed in this regard. To this end we employed the single molecule in situ hybridization technique RNAscope on sensory neurons immediately after detection of calcium responses to the TRPA1 agonist allyl isothiocyanate. There is a clearly positive correlation between TRPA1 calcium imaging and RNAscope detection ( R = 0.43), although less than what might have been expected. Thus, the technique of choice should be carefully considered to suit the research question. The marginal correlation between TRPV1 RNAscope and the specific agonist capsaicin indicates that such validation is advisable for every RNAscope target. Given the recent description of a long-awaited TRPA1 reporter mouse, TRPA1 RNAscope detection might still have its use cases, for detection of RNA at particular sites, for example, defined structurally or by other molecular markers.<\/jats:p>","DOI":"10.1369\/00221554241251904","type":"journal-article","created":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T03:26:20Z","timestamp":1715311580000},"page":"275-287","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Correlation of TRPA1 RNAscope and Agonist Responses"],"prefix":"10.1369","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1432-9336","authenticated-orcid":false,"given":"Natalia S.","family":"Rojas-Galvan","sequence":"first","affiliation":[{"name":"Centre for Physiology and Pharmacology, Medical University of Vienna, Vienna, Austria and Randall Centre for Cell & Molecular Biophysics, King\u2019s College London, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6832-0647","authenticated-orcid":false,"given":"Cosmin I.","family":"Ciotu","sequence":"additional","affiliation":[{"name":"Centre for Physiology and Pharmacology, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3398-0442","authenticated-orcid":false,"given":"Stefan","family":"Heber","sequence":"additional","affiliation":[{"name":"Centre for Physiology and Pharmacology, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3811-7066","authenticated-orcid":false,"given":"Michael J.M.","family":"Fischer","sequence":"additional","affiliation":[{"name":"Centre for Physiology and Pharmacology, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,5,10]]},"reference":[{"key":"bibr1-00221554241251904","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00158-2"},{"key":"bibr2-00221554241251904","volume":"121","author":"Meents JE","year":"2018","journal-title":"J Neurophysiol"},{"key":"bibr3-00221554241251904","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0047988"},{"key":"bibr4-00221554241251904","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0505356102"},{"key":"bibr5-00221554241251904","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20794"},{"key":"bibr6-00221554241251904","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3048-18.2019"},{"key":"bibr7-00221554241251904","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.11.7325"},{"key":"bibr8-00221554241251904","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2016-0089OC"},{"key":"bibr9-00221554241251904","doi-asserted-by":"publisher","DOI":"10.1523\/ENEURO.0350-23.2023"},{"key":"bibr10-00221554241251904","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.189530"},{"key":"bibr11-00221554241251904","doi-asserted-by":"crossref","unstructured":"Souza Monteiro de Ara\u00fajo D, De Logu F, Adembri C, Rizzo S, Janal MN, Landini L, Magi A, Mattei G, Cini N, Pandolfo P, Geppetti P, Nassini R, Da Costa Calaza K. 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