{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T05:31:03Z","timestamp":1790659863390,"version":"4.1.0"},"reference-count":34,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["22K06977"],"award-info":[{"award-number":["22K06977"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["21H02702"],"award-info":[{"award-number":["21H02702"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:p>Summary<\/jats:p>\n                  <jats:p>We previously demonstrated that among various histological types of human testicular germinal cell tumors (GCTs), embryonal carcinoma (EC) preferentially expresses low-sulfated keratan sulfate (KS) consisting of repeating N-acetyllactosamine (LacNAc) disaccharide units composed of galactose and 6- O-sulfated N-acetylglucosamine (GlcNAc), which is recognized by the R-10G antibody. Recently, we generated another anti-low-sulfated KS monoclonal antibody, 294-1B1. Immunohistochemical analysis of testicular GCTs ( n=83) revealed that the low-sulfated KS recognized by 294-1B1 is also preferentially expressed in EC but minimally in other GCT histological types. Moreover, immunolabeling with R-10G and 294-1B1 antibodies was resistant to peptide- N-glycosidase F digestion, and EC was not stained with the MECA-79 antibody, indicating that low-sulfated KS expressed in EC contains mucin-type core 2 O-glycans carrying GlcNAc-6- O-sulfated oligo-LacNAc. Double immunofluorescence staining showed that R-10G and 294-1B1 antibody signals colocalized with those for podocalyxin (PODXL). Furthermore, western blot analysis of recombinant human PODXL\u2022IgG fusion proteins secreted from low-sulfated KS-expressing human embryonic kidney 293T cells revealed that PODXL functions as a core protein for low-sulfated KS. Taken together, these findings strongly suggest that the PODXL glycoform decorated with low-sulfated KS is preferentially expressed in human testicular EC and may therefore serve as a diagnostic marker for this malignancy<\/jats:p>","DOI":"10.1369\/00221554241265162","type":"journal-article","created":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T07:06:21Z","timestamp":1721891181000},"page":"453-465","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Expression of Podocalyxin Potentially Decorated With Low-sulfated Keratan Sulfate in Human Testicular Embryonal Carcinoma"],"prefix":"10.1369","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6622-5638","authenticated-orcid":false,"given":"Akifumi","family":"Muramoto","sequence":"first","affiliation":[{"name":"Department of Tumor Pathology, Faculty of Medical Sciences, University of Fukui, Eiheiji, Japan"},{"name":"Division of Surgical Pathology, University of Fukui Hospital, Eiheiji, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitomi","family":"Hoshino","sequence":"additional","affiliation":[{"name":"Department of Tumor Pathology, Faculty of Medical Sciences, University of Fukui, Eiheiji, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1261-5059","authenticated-orcid":false,"given":"So","family":"Inamura","sequence":"additional","affiliation":[{"name":"Department of Tumor Pathology, Faculty of Medical Sciences, University of Fukui, Eiheiji, Japan"},{"name":"Department of Urology, Faculty of Medical Sciences, University of Fukui, Eiheiji, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1158-7375","authenticated-orcid":false,"given":"Masataka","family":"Murahashi","sequence":"additional","affiliation":[{"name":"Department of Tumor Pathology, Faculty of Medical Sciences, University of Fukui, Eiheiji, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4248-6929","authenticated-orcid":false,"given":"Tomoya O.","family":"Akama","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Kansai Medical University, Hirakata, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3591-1715","authenticated-orcid":false,"given":"Naoki","family":"Terada","sequence":"additional","affiliation":[{"name":"Department of Urology, Faculty of Medical Sciences, University of Fukui, Eiheiji, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7607-0801","authenticated-orcid":false,"given":"Motohiro","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Tumor Pathology, Faculty of Medical Sciences, University of Fukui, Eiheiji, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,7,25]]},"reference":[{"key":"bibr1-00221554241265162","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/10.10.951"},{"key":"bibr2-00221554241265162","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)49203-4"},{"key":"bibr3-00221554241265162","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.37.26165"},{"key":"bibr4-00221554241265162","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-009-0228-7"},{"key":"bibr5-00221554241265162","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwy003"},{"key":"bibr6-00221554241265162","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwad056"},{"issue":"14","key":"bibr7-00221554241265162","volume":"258","author":"Caterson B","year":"1983","journal-title":"Monoclonal antibodies to cartilage proteoglycan. 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