{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T07:31:48Z","timestamp":1791617508888,"version":"4.3.4"},"reference-count":9,"publisher":"SAGE Publications","issue":"9-10","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:p>Probably the most sensitive and frequently performed stain for calcium deposits in mammalian tissues is the von Kossa, a stain used for over a century. When originally incorporated into daily use in the histopathology laboratory on paraffin tissue sections, the procedure called for immersion of tissue sections in an aqueous 5% silver nitrate solution and placed in direct sunlight for 20\u201330 min to make calcium deposits appear black. Although this effective method continues to be used in select locations, the modern laboratory often does not have access to bright sunlight. This study revisited the traditional von Kossa staining method with alternatives in light sources, silver nitrate concentrations, and timing of silver solution exposure and determined that a 1% silver nitrate solution is equally effective as a 5% solution in bright sunlight for 30\u201360 min. In addition, a 1% silver nitrate solution can be successfully used with exposure to a 3.5W light-emitting diode (LED) daylight lamp for 1 hr, sunlight through a tinted glass window for 1 hr, or overhead room lighting for 2 hr, and the staining reaction is unaffected by temperatures ranging from 1C to 40C:<\/jats:p>","DOI":"10.1369\/00221554251379535","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T09:25:37Z","timestamp":1758792337000},"page":"339-344","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Von Kossa Calcium Staining Procedure Revisited"],"prefix":"10.1369","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1446-2156","authenticated-orcid":false,"given":"Sheila","family":"Criswell","sequence":"first","affiliation":[{"name":"Department of Diagnostic and Health Sciences, College of Health Professions, The University of Tennessee Health Science Center, Memphis, Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,9,25]]},"reference":[{"key":"e_1_3_3_2_2","first-page":"163","article-title":"Ueber die im Organismus k\u00fcnstlich erzeugbaren Verkalkungen","volume":"29","author":"von Kossa J","year":"1901","unstructured":"von Kossa J. Ueber die im Organismus k\u00fcnstlich erzeugbaren Verkalkungen. Beitr Pathol Anat Allg Pathol. 1901;29:163\u2013202.","journal-title":"Beitr Pathol Anat Allg Pathol"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-021-02051-3"},{"key":"e_1_3_3_4_2","unstructured":"Carson F Hladik C. Histotechnology a self-instructional text third edition. Hong Kong: American Society of Clinical Pathology Press."},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00495978"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1179\/his.1985.8.1.11"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1177\/17.2.110"},{"key":"e_1_3_3_8_2","unstructured":"Photography. In: Chisholm H editor. Encyclopaedia britannica. 11th ed. vol. 21. Cambridge: Cambridge University Press; 1911. p. 732\u201348."},{"key":"e_1_3_3_9_2","unstructured":"Drury RAB Wallington EA editors. Carleton\u2019s histologic technique. 5th ed. 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