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A transmural, non-reperfused MI was induced in middle-aged male Sprague-Dawley rats via left anterior coronary artery ligation, and proliferating cells were labeled with 5-bromo-2\u2032-deoxyuridine. Hearts were collected at days 3, 7, and 14 after MI and analyzed using histology and immunohistochemistry. We found that the myocardial interstitium and coronary vessel adventitia harbored a population of cardiac CD34\u207a SCs. Following MI, activated CD34\u207a SCs expanded from the peri-infarct region across the healing wound through proliferation and migration, often alongside activated fibroblasts\/myofibroblasts. While \u03b1-SMA\u207a myofibroblasts accumulated at pro-fibrotic granulation tissue sites, CD34\u207a SCs preferentially repopulated residual endomysial scaffolds spared by phagocytic macrophages. Over time, expanding fibrotic tissue progressively overtook these regions, leading to disappearance of CD34\u207a SCs. Importantly, clusters of CD34\u207a SCs accumulated at the scar border around the stumps of surviving cardiac myocytes, seemingly facilitating integration of endomysial connective tissue from non-infarcted myocardium into the developing fibrotic scar matrix. Collectively, these findings suggest that, unlike \u03b1-SMA\u207a myofibroblasts, cardiac CD34\u207a SCs seemed to support regenerative rather than fibrotic repair during post-MI wound healing by contributing to the preservation of myocardial stromal architecture.<\/jats:p>","DOI":"10.1369\/00221554261434317","type":"journal-article","created":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T16:58:11Z","timestamp":1776013091000},"page":"285-310","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Contribution of Cardiac CD34\u207a Stromal Cells to Post-Myocardial Infarction Repair in Middle-Aged Rats"],"prefix":"10.1369","volume":"74","author":[{"given":"Daniel T.","family":"Schneider","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Cooper Medical School of Rowan University, Camden, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7309-7662","authenticated-orcid":false,"given":"Eduard I.","family":"Dedkov","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Cooper Medical School of Rowan University, Camden, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2026,4,12]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/ehf2.13144"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.25745"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.121.318172"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehac223"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2023.12.009"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.2174\/1874471013666201210140743"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c140067"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.303577"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2023.01.013"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2023.12.001"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2023.07.009"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2015.11.002"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22031201"},{"issue":"2","key":"e_1_3_3_15_2","article-title":"The dynamic facets of the cardiac stroma: from classical markers to omics and translational perspectives","volume":"14","author":"Picchio V","year":"2022","unstructured":"Picchio V, Bordin A, Floris E, Cozzolino C, Dhori X, Peruzzi M, Frati G, De Falco E, Pagano F, Chimenti I. 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