{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:51:34Z","timestamp":1792788694397,"version":"4.5.0"},"reference-count":45,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Ministry of Education, Culture and Science of the Netherlands,","award":["024.006.034"],"award-info":[{"award-number":["024.006.034"]}],"id":[{"id":"https:\/\/ror.org\/02x3w5g21","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Stichting Steunfonds Uitzicht","award":["UZ2022-25"],"award-info":[{"award-number":["UZ2022-25"]}],"id":[{"id":"https:\/\/ror.org\/05r1tfa82","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Diabetes Fonds","award":["2022.22.009"],"award-info":[{"award-number":["2022.22.009"]}],"id":[{"id":"https:\/\/ror.org\/04ch2g225","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:p>\n                    Microvascular dysfunction due to hypoxia is a key contributor in the pathogenesis of many disorders including cancer and retinal and cardiovascular diseases, but relevant human models are missing. Here, we present a robust 3D in vitro method with the use of human induced pluripotent stem cell\u2013derived blood vessel organoids to analyze in vitro microvascular remodeling. We present a detailed practical pipeline combining optical tissue clearing, high-resolution immunofluorescence, and surface marker analysis to quantitatively assess hypoxia-driven changes in endothelial cells, pericytes, and the basal lamina. Exposure of these blood vessel organoids to chronic hypoxia (1% O\n                    <jats:sub>2<\/jats:sub>\n                    ) for 1 week recapitulated key pathological features, including structural remodeling and a dysregulated secretome with altered vascular endothelial growth factor signaling. This approach establishes a versatile and human-relevant platform to study microvascular remodeling induced by chronic hypoxia and other pathological stimuli and their contribution to microvascular-related diseases:\n                  <\/jats:p>","DOI":"10.1369\/00221554261437861","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T11:27:47Z","timestamp":1778498867000},"page":"423-434","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Human iPSC-Derived Blood Vessel Organoids for Studying Chronic Hypoxia-Induced Microvascular Dysfunction"],"prefix":"10.1369","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0688-0083","authenticated-orcid":false,"given":"Paola","family":"Serrano Martinez","sequence":"first","affiliation":[{"name":"Ocular Angiogenesis Group, Department of Ophthalmology, Amsterdam UMC location University of Amsterdam, Amsterdam, The Netherlands"},{"name":"Microcirculation, Amsterdam Cardiovascular Sciences, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxime","family":"Cammeraat","sequence":"additional","affiliation":[{"name":"Ocular Angiogenesis Group, Department of Ophthalmology, Amsterdam UMC location University of Amsterdam, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amber","family":"Teppema","sequence":"additional","affiliation":[{"name":"Ocular Angiogenesis Group, Department of Ophthalmology, Amsterdam UMC location University of Amsterdam, Amsterdam, The Netherlands"},{"name":"Microcirculation, Amsterdam Cardiovascular Sciences, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6191-0505","authenticated-orcid":false,"given":"Cornelis J. 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