{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T09:45:42Z","timestamp":1764841542273,"version":"3.34.0"},"reference-count":46,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2006,9,1]],"date-time":"2006-09-01T00:00:00Z","timestamp":1157068800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:p> The skeleton of demosponges is built of spicules consisting of biosilica. Using the primmorph system from Suberites domuncula, we demonstrate that silicatein, the biosilica-synthesizing enzyme, and silicase, the catabolic enzyme, are colocalized at the surface of growing spicules as well as in the axial filament located in the axial canal. It is assumed that these two enzymes are responsible for the deposition of biosilica. In search of additional potential structural molecules that might guide the mineralization process during spiculogenesis to species-specific spicules, electron microscopic studies with antibodies against galectin and silicatein were performed. These studies showed that silicatein forms a complex with galectin; the strings\/bundles of this complex are intimately associated with the surface of the spicules and arranged concentrically around them. Collagen fibers are near the silactein\/galectin complexes. The strings\/bundles formed from silicatein\/galectin display a lower degree of orientation than the collagen fibers arranged in a highly ordered pattern around the spicules. These data indicate that species-specific formation of spicules involves a network of (diffusible) regulatory factor(s) controlling enzymatic silica deposition; this mineralization process proceeds on a galectin\/collagen organic matrix. <\/jats:p>","DOI":"10.1369\/jhc.5a6903.2006","type":"journal-article","created":{"date-parts":[[2006,5,19]],"date-time":"2006-05-19T00:58:28Z","timestamp":1148000308000},"page":"1031-1040","source":"Crossref","is-referenced-by-count":46,"title":["Histochemical and Electron Microscopic Analysis of Spiculogenesis in the Demosponge <i>Suberites domuncula<\/i>"],"prefix":"10.1177","volume":"54","author":[{"given":"Carsten","family":"Eckert","sequence":"first","affiliation":[{"name":"Museum f\u00fcr Naturkunde, Universit\u00e4t, Institut f\u00fcr Systematische Zoologie, Berlin, Germany"}]},{"given":"Heinz C.","family":"Schr\u00f6der","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Physiologische Chemie, Abteilung Angewandte Molekularbiologie, Universit\u00e4t, Mainz, Germany"}]},{"given":"David","family":"Brandt","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Physiologische Chemie, Abteilung Angewandte Molekularbiologie, Universit\u00e4t, Mainz, Germany"}]},{"given":"Sanja","family":"Perovic-Ottstadt","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Physiologische Chemie, Abteilung Angewandte Molekularbiologie, Universit\u00e4t, Mainz, Germany"}]},{"given":"Werner E.G.","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Physiologische Chemie, Abteilung Angewandte Molekularbiologie, Universit\u00e4t, Mainz, Germany"}]}],"member":"179","published-online":{"date-parts":[[2006,9,1]]},"reference":[{"volume-title":"Sponges","year":"1978","author":"Bergquist PR","key":"bibr1-jhc.5A6903.2006"},{"key":"bibr2-jhc.5A6903.2006","doi-asserted-by":"crossref","DOI":"10.5479\/si.00810282.596","volume-title":"Thesaurus of Sponge Morphology","author":"Boury-Esnault N","year":"1997"},{"key":"bibr3-jhc.5A6903.2006","first-page":"235","volume":"64","author":"B\u00fctschli O","year":"1901","journal-title":"Z Wiss Zool"},{"key":"bibr4-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.2.361"},{"key":"bibr5-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1007\/BF00592566"},{"key":"bibr6-jhc.5A6903.2006","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1242\/jcs.79.1.271","volume":"79","author":"Diehl-Seifert B","year":"1985","journal-title":"J Cell Sci"},{"volume-title":"Oral Cells and Tissues","year":"2003","author":"Garant PR","key":"bibr7-jhc.5A6903.2006"},{"volume-title":"Phylogenesis of Connective Tissue","year":"1978","author":"Garrone R","key":"bibr8-jhc.5A6903.2006"},{"key":"bibr9-jhc.5A6903.2006","first-page":"461","volume-title":"The Role of Extracellular Matrix in Development","author":"Garrone R","year":"1984"},{"key":"bibr10-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-72236-3_6"},{"key":"bibr11-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.2307\/1541014"},{"key":"bibr12-jhc.5A6903.2006","first-page":"1","volume":"5","author":"Ijima I","year":"1901","journal-title":"J Coll Sci Imp Univ Tokyo"},{"key":"bibr13-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1007\/s00114-004-0599-4"},{"key":"bibr14-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2000.01547.x"},{"key":"bibr15-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1089\/10445490252810320"},{"key":"bibr16-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a025742"},{"key":"bibr17-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-2760(98)00005-8"},{"key":"bibr18-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1016\/S0898-6568(98)00059-X"},{"key":"bibr19-jhc.5A6903.2006","first-page":"171","volume":"2","author":"Minchin EA","year":"1909","journal-title":"Ergebn Fortschr Zoologie"},{"key":"bibr20-jhc.5A6903.2006","first-page":"269","volume-title":"Encyclopedia of Molecular Cell Biology and Molecular Medicine","volume":"13","author":"M\u00fcller WEG","year":"2005"},{"key":"bibr21-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1016\/j.micron.2005.09.003"},{"key":"bibr22-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.4052\/tigg.9.123"},{"key":"bibr23-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-55486-5_8"},{"key":"bibr24-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-005-1141-5"},{"key":"bibr25-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.3354\/meps178205"},{"key":"bibr26-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1046\/j.1525-142X.2003.03023.x"},{"volume-title":"In Situ Hybridization","year":"1998","author":"Polak JM","key":"bibr27-jhc.5A6903.2006"},{"key":"bibr28-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.11.076"},{"key":"bibr29-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M512677200"},{"key":"bibr30-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-55486-5_10"},{"key":"bibr31-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20040463"},{"key":"bibr32-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-004-0869-7"},{"key":"bibr33-jhc.5A6903.2006","first-page":"513","volume-title":"Report on the Scientific Results of the Voyage of the HMS Challenger during the Years 1873-1876","volume":"21","author":"Schulze FE","year":"1887"},{"key":"bibr34-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.5962\/bhl.title.85181"},{"volume-title":"Die Bezeichnung der Spongiennadeln","year":"1889","author":"Schulze FE","key":"bibr35-jhc.5A6903.2006"},{"key":"bibr36-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.11.6234"},{"key":"bibr37-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051860310"},{"key":"bibr38-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-5214-6"},{"key":"bibr39-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1007\/s004410000234"},{"key":"bibr40-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.10395"},{"key":"bibr41-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.10401"},{"key":"bibr42-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.2113\/0540001"},{"volume-title":"Biologie und Mikroskopische Anatomie der S\u00fc\u00dfwasserschw\u00e4mme (Spongillidae)","year":"1989","author":"Weissenfels N","key":"bibr43-jhc.5A6903.2006"},{"key":"bibr44-jhc.5A6903.2006","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0021-9258(18)91582-6","volume":"7","author":"Wheeler HL","year":"1909","journal-title":"J Biol Chem"},{"key":"bibr45-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2006.25.152"},{"key":"bibr46-jhc.5A6903.2006","doi-asserted-by":"publisher","DOI":"10.1007\/s00239-003-0008-1"}],"container-title":["Journal of Histochemistry &amp; Cytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/jhc.5A6903.2006","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1369\/jhc.5A6903.2006","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/jhc.5A6903.2006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T10:09:30Z","timestamp":1738058970000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1369\/jhc.5A6903.2006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,9]]},"references-count":46,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2006,9]]}},"alternative-id":["10.1369\/jhc.5A6903.2006"],"URL":"https:\/\/doi.org\/10.1369\/jhc.5a6903.2006","relation":{},"ISSN":["0022-1554","1551-5044"],"issn-type":[{"type":"print","value":"0022-1554"},{"type":"electronic","value":"1551-5044"}],"subject":[],"published":{"date-parts":[[2006,9]]}}}