{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T23:20:07Z","timestamp":1788996007136,"version":"build-2803163510"},"reference-count":24,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2008,12,24]],"date-time":"2008-12-24T00:00:00Z","timestamp":1230076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:p>Immunoglobulin (Ig) molecules have thus far been found only to be produced by differentiated B lymphocytes. As we know, immune privilege in the normal adult mouse testis exists to make these privileged sites generally free of patrolling immune cells and Igs. However, in this study, the expression of Ig in normal adult mouse testis and epididymis was detected. First, by IHC, a strong pattern of Ig expression was detected in the spermatogenic cells of adult mouse testis at different development stages. Second, by Western blot analysis, different strong bands of Igs in mouse testicular spermatogenic cells and epididymal epithelial cell extract were recognized using specific antibodies against IgG. More importantly, by ISH and cell sorting\u2013related RT-PCR, rearranged Ig \u03b3 chain and \u03ba chain transcripts were expressed in testicular spermatogenic cells and epididymal epithelial cells. These results suggested that Ig in testis and epididymis was mainly produced by adult mouse testicular spermatogenic cells and epididymal epithelial cells. This manuscript contains online supplemental material at http:\/\/www.jhc.org. Please visit this article online to view these materials.<\/jats:p>","DOI":"10.1369\/jhc.2008.951434","type":"journal-article","created":{"date-parts":[[2008,12,8]],"date-time":"2008-12-08T20:48:29Z","timestamp":1228769309000},"page":"339-349","source":"Crossref","is-referenced-by-count":49,"title":["Expression of Immunoglobulin Gene With Classical V-(D)-J Rearrangement in Mouse Testis and Epididymis"],"prefix":"10.1177","volume":"57","author":[{"given":"Jing","family":"Huang","sequence":"first","affiliation":[{"name":"Center for Human Disease Genomics, Institution of Basic Medical Science, Peking University, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Human Disease Genomics, Institution of Basic Medical Science, Peking University, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teng","family":"Ma","sequence":"additional","affiliation":[{"name":"Center for Human Disease Genomics, Institution of Basic Medical Science, Peking University, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Human Disease Genomics, Institution of Basic Medical Science, Peking University, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Qiu","sequence":"additional","affiliation":[{"name":"Center for Human Disease Genomics, Institution of Basic Medical Science, Peking University, Beijing, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2008,12,24]]},"reference":[{"key":"bibr1-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402030209"},{"key":"bibr2-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-3704"},{"key":"bibr3-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20129"},{"key":"bibr4-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0378(92)90032-Y"},{"key":"bibr5-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/17.4.984"},{"key":"bibr6-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2006.00438.x"},{"key":"bibr7-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0897.2001.d01-28.x"},{"key":"bibr8-jhc.2008.951434","first-page":"289","volume":"12","author":"Gurevich P","year":"2003","journal-title":"Int J Mol Med"},{"key":"bibr9-jhc.2008.951434","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1002\/j.1939-4640.1994.tb00502.x","volume":"15","author":"Hedger MP","year":"1994","journal-title":"J Androl"},{"key":"bibr10-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0378(02)00060-8"},{"key":"bibr11-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.04.021"},{"key":"bibr12-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1996.tb00165.x"},{"key":"bibr13-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2007.12.004"},{"key":"bibr14-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2005.06.007"},{"key":"bibr15-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.3109\/01485019108987645"},{"key":"bibr16-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.105.041079"},{"key":"bibr17-jhc.2008.951434","first-page":"443","volume":"7","author":"Lenzi A","year":"2001","journal-title":"Hum Reprod Update"},{"key":"bibr18-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2006.07.012"},{"key":"bibr19-jhc.2008.951434","first-page":"6488","volume":"63","author":"Qiu X","year":"2003","journal-title":"Cancer Res"},{"key":"bibr20-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.1002\/path.2014"},{"key":"bibr21-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.11.6790"},{"key":"bibr22-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-03-0341"},{"key":"bibr23-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2006.05.001"},{"key":"bibr24-jhc.2008.951434","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2006.08.012"}],"container-title":["Journal of Histochemistry &amp; 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