{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T12:35:04Z","timestamp":1789734904244,"version":"4.0.1"},"reference-count":18,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:p>Helicobacter pylori ( H. pylori) is the causative pathogen underlying gastric diseases such as chronic gastritis and gastric cancer. Previously, the authors revealed that \u03b11,4-linked N-acetylglucosamine-capped O-glycan (\u03b1GlcNAc) found in gland mucin suppresses H. pylori growth and motility by inhibiting catalytic activity of cholesterol \u03b1-glucosyltransferase (CHL\u03b1GcT), the enzyme responsible for biosynthesis of the major cell wall component cholesteryl-\u03b1-d-glucopyranoside (CGL). Here, the authors developed a polyclonal antibody specific for CHL\u03b1GcT and then undertook quantitative ultrastructural analysis of the enzyme\u2019s localization in H. pylori. They show that 66.3% of CHL\u03b1GcT is detected in the cytoplasm beneath the H. pylori inner membrane, whereas 24.7% is present on the inner membrane. In addition, 2.6%, 5.0%, and 1.4% of the protein were detected in the periplasm, on the outer membrane, and outside microbes, respectively. By using an in vitro CHL\u03b1GcT assay with fractionated H. pylori proteins, which were used as an enzyme source for CHL\u03b1GcT, the authors demonstrated that the membrane fraction formed CGL, whereas other fractions did not. These data combined together indicate that CHL\u03b1GcT is originally synthesized in the cytoplasm of H. pylori as an inactive form and then activated when it is associated with the cell membrane. This article contains online supplemental material at http:\/\/www.jhc.org . Please visit this article online to view these materials.<\/jats:p>","DOI":"10.1369\/jhc.2010.957092","type":"journal-article","created":{"date-parts":[[2010,9,28]],"date-time":"2010-09-28T00:59:15Z","timestamp":1285635555000},"page":"98-105","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":29,"title":["Membrane-associated Activation of Cholesterol \u03b1-Glucosyltransferase, an Enzyme Responsible for Biosynthesis of Cholesteryl-\u03b1-\n                    <scp>d<\/scp>\n                    -Glucopyranoside in\n                    <i>Helicobacter pylori<\/i>\n                    Critical for Its Survival"],"prefix":"10.1177","volume":"59","author":[{"given":"Hitomi","family":"Hoshino","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiko","family":"Tsuchida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyokazu","family":"Kametani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masako","family":"Mori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoko","family":"Nishizawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takefumi","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitomi","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heeseob","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, Pusan National University, Jangjeon-dong, Geumjeong-gu, Busan 609-735, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Ito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motohiro","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junya","family":"Masumoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaya","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minoru","family":"Fukuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Nakayama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2011,2,23]]},"reference":[{"key":"bibr1-jhc.2010.957092","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1988.tb13820.x"},{"key":"bibr2-jhc.2010.957092","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-5378.2008.00631.x"},{"key":"bibr3-jhc.2010.957092","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.34.21357"},{"key":"bibr4-jhc.2010.957092","doi-asserted-by":"publisher","DOI":"10.1136\/gut.49.4.474"},{"key":"bibr5-jhc.2010.957092","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.18.5327-5333.1995"},{"key":"bibr6-jhc.2010.957092","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5349.373"},{"key":"bibr7-jhc.2010.957092","doi-asserted-by":"publisher","DOI":"10.1177\/002215540305100502"},{"key":"bibr8-jhc.2010.957092","doi-asserted-by":"publisher","DOI":"10.1126\/science.1099250"},{"key":"bibr9-jhc.2010.957092","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M603345200"},{"key":"bibr10-jhc.2010.957092","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.08.145"},{"key":"bibr11-jhc.2010.957092","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwn037"},{"key":"bibr12-jhc.2010.957092","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M301704200"},{"key":"bibr13-jhc.2010.957092","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(84)91816-6"},{"key":"bibr14-jhc.2010.957092","doi-asserted-by":"publisher","DOI":"10.1038\/nrc703"},{"key":"bibr15-jhc.2010.957092","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00153.x"},{"key":"bibr16-jhc.2010.957092","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2818.2003.01225.x"},{"key":"bibr17-jhc.2010.957092","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-5378.2003.00173.x"},{"key":"bibr18-jhc.2010.957092","doi-asserted-by":"publisher","DOI":"10.1038\/nm1480"}],"container-title":["Journal of Histochemistry &amp; 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