{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T23:43:26Z","timestamp":1777851806263,"version":"3.51.4"},"reference-count":23,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:p>Genetic immunization can be combined with hybridoma technology to generate high-affinity monoclonal antibodies (MAbs). A new anti-BCL-6 MAb (GI191E\/A8) was produced by cloning full-length BCL-6 cDNA into a eukaryotic vector and delivering this into mouse epidermis using a helium gene gun. A comparative study was made of the specificity and the effects of formalin fixation on immunohistochemistry quality of GI191E\/A8 and two other anti-BCL-6 MAbs. To evaluate its possible application to differential diagnosis of lymphomas, two tissue microarrays (89 diffuse large B-cell lymphomas and 24 B-cell chronic lymphocytic leukemia cases) were stained with GI191E\/A8 and another anti-BCL-6 MAb produced by conventional means. Using GI191E\/A8, the detection of BCL-6 protein was significantly increased, and its specificity was independent of formalin-fixation time. Using automatic quantified analysis, the correlation between the two anti-BCL-6 MAbs tested was identical in cases with overexpression or absence of BCL-6. In cases with intermediate BCL-6 protein expression, detection with GI191E\/A8 was more sensitive. A significant association of higher BCL-6 expression and longer median overall survival times in diffuse large B-cell lymphomas was found. Using conventionally produced MAbs in the same patient group, the association was not significant.<\/jats:p>","DOI":"10.1369\/jhc.5a6646.2005","type":"journal-article","created":{"date-parts":[[2005,7,26]],"date-time":"2005-07-26T22:44:33Z","timestamp":1122417873000},"page":"31-38","source":"Crossref","is-referenced-by-count":11,"title":["Genetic Immunization: A New Monoclonal Antibody for the Detection of BCL-6 Protein in Paraffin Sections"],"prefix":"10.1177","volume":"54","author":[{"given":"Jos\u00e9-Francisco","family":"Garc\u00eda","sequence":"first","affiliation":[{"name":"Monoclonal Antibodies Unit (J-FG, LM, EL, GR)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan-Fernando","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Monoclonal Antibodies Unit (J-FG, LM, EL, GR)"},{"name":"Biotechnology Program, Molecular Pathology Program (J-FG, M-AP), Spanish National Cancer Centre (CNIO), Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorena","family":"Maestre","sequence":"additional","affiliation":[{"name":"Monoclonal Antibodies Unit (J-FG, LM, EL, GR)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Lucas","sequence":"additional","affiliation":[{"name":"Monoclonal Antibodies Unit (J-FG, LM, EL, GR)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lydia","family":"S\u00e1nchez-Verde","sequence":"additional","affiliation":[{"name":"Histology and Immunochemistry Unit (LS-V)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Romero-Chala","sequence":"additional","affiliation":[{"name":"Protein Technology Unit (SR-C)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel-\u00c1ngel","family":"Piris","sequence":"additional","affiliation":[{"name":"Biotechnology Program, Molecular Pathology Program (J-FG, M-AP), Spanish National Cancer Centre (CNIO), Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanna","family":"Roncador","sequence":"additional","affiliation":[{"name":"Monoclonal Antibodies Unit (J-FG, LM, EL, GR)"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2006,1,1]]},"reference":[{"key":"bibr1-jhc.5A6646.2005","first-page":"616","volume":"16","author":"Barry MA","year":"1994","journal-title":"Biotechniques"},{"key":"bibr2-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1038\/nbt858"},{"key":"bibr3-jhc.5A6646.2005","doi-asserted-by":"crossref","first-page":"1458","DOI":"10.4049\/jimmunol.160.3.1458","volume":"160","author":"Costagliola S","year":"1998","journal-title":"J Immunol"},{"key":"bibr4-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(84)91451-X"},{"key":"bibr5-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.2.409"},{"key":"bibr6-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-04-1128"},{"key":"bibr7-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-71-7-1481"},{"key":"bibr8-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.18.1.927"},{"key":"bibr9-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1038\/sj.thj.6200013"},{"key":"bibr10-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.7.657"},{"key":"bibr11-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1089\/hyb.1998.17.569"},{"key":"bibr12-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1089\/027245700429846"},{"key":"bibr13-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1089\/153685902760213840"},{"key":"bibr14-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1656(99)00115-7"},{"key":"bibr15-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.4.945"},{"key":"bibr16-jhc.5A6646.2005","first-page":"175","volume-title":"Techniques in Immunocytochemistry","author":"Mason DY","year":"1983"},{"key":"bibr17-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(03)00192-3"},{"key":"bibr18-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-07-2356"},{"key":"bibr19-jhc.5A6646.2005","doi-asserted-by":"crossref","first-page":"4541","DOI":"10.1128\/JVI.72.5.4541-4545.1998","volume":"72","author":"Schmolke S","year":"1998","journal-title":"J Virol"},{"key":"bibr20-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1007\/BF00571929"},{"key":"bibr21-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1656(96)01610-0"},{"key":"bibr22-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200007000-00014"},{"key":"bibr23-jhc.5A6646.2005","doi-asserted-by":"publisher","DOI":"10.1038\/ng0697-161"}],"container-title":["Journal of Histochemistry &amp; 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