{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T23:44:01Z","timestamp":1777851841934,"version":"3.51.4"},"reference-count":44,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2006,9,1]],"date-time":"2006-09-01T00:00:00Z","timestamp":1157068800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:p>\n                    This work was directed to evaluate immunoexpression of markers for apoptosis, resistance to apoptosis, and cell proliferation, as well as estimates of nuclear size in ventral prostate of rats treated with cadmium chloride and cadmium + zinc chloride because a possible protective effect of zinc has been postulated. The following variables were studied: volume fraction (V\n                    <jats:sub>F<\/jats:sub>\n                    ) of Bcl-2 immunostaining, percentage of cells immunoreactive to proliferating cell nuclear antigen (LI\n                    <jats:sub>PCNA<\/jats:sub>\n                    ) and p53 (LI\n                    <jats:sub>p53<\/jats:sub>\n                    ), numerical density of caspase-3 immunoreactive cells (N\n                    <jats:sub>V<\/jats:sub>\n                    caspase-3), and estimates of volume-weighted mean nuclear volume (\u03c5\n                    <jats:sub>V<\/jats:sub>\n                    ). The LI\n                    <jats:sub>PCNA<\/jats:sub>\n                    and V\n                    <jats:sub>F<\/jats:sub>\n                    of Bcl-2 were significantly increased in the treated animals. The dysplasias (independent of their origin) showed a significant increase of the LI\n                    <jats:sub>p53<\/jats:sub>\n                    , N\n                    <jats:sub>V<\/jats:sub>\n                    caspase-3, and \u03c5\n                    <jats:sub>V<\/jats:sub>\n                    in comparison with normal acini from treated and control animals. It can be concluded that cell proliferation is enhanced in long-term cadmium-exposed rats, and exposure to zinc combined with cadmium had no effect on any of the variables studied when comparing with normal acini. The increase of nuclear \u03c5\n                    <jats:sub>V<\/jats:sub>\n                    could indicate a more aggressive behavior for pretumoral lesions.\n                  <\/jats:p>","DOI":"10.1369\/jhc.5a6733.2006","type":"journal-article","created":{"date-parts":[[2006,4,3]],"date-time":"2006-04-03T20:33:58Z","timestamp":1144096438000},"page":"981-990","source":"Crossref","is-referenced-by-count":22,"title":["Immunohistochemical Study of Cell Proliferation, Bcl-2, p53, and Caspase-3 Expression on Preneoplastic Changes Induced by Cadmium and Zinc Chloride in the Ventral Rat Prostate"],"prefix":"10.1177","volume":"54","author":[{"given":"Ri\u00e1nsares","family":"Arriazu","sequence":"first","affiliation":[{"name":"Cell Biology and Histology Laboratory, San Pablo-CEU University, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 M.","family":"Pozuelo","sequence":"additional","affiliation":[{"name":"Cell Biology and Histology Laboratory, San Pablo-CEU University, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nuno","family":"Henriques-Gil","sequence":"additional","affiliation":[{"name":"Genetics Laboratory, San Pablo-CEU University, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Perucho","sequence":"additional","affiliation":[{"name":"Genetics Laboratory, San Pablo-CEU University, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roc\u00edo","family":"Mart\u00edn","sequence":"additional","affiliation":[{"name":"Service of Pathology, Hospital N. Sra. de Sonsoles, \u00c1vila, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosario","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Cell Biology and Histology Laboratory, San Pablo-CEU University, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis","family":"Santamar\u00eda","sequence":"additional","affiliation":[{"name":"Department of Morphology, School of Medicine, Autonomous University of Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2006,9,1]]},"reference":[{"key":"bibr1-jhc.5A6733.2006","first-page":"455","volume":"61","author":"Achanzar WE","year":"2001","journal-title":"Cancer Res"},{"key":"bibr2-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0045(20010201)46:2<134::AID-PROS1017>3.0.CO;2-9"},{"key":"bibr3-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1002\/pros.20192"},{"key":"bibr4-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-23761-5_58"},{"key":"bibr5-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1159\/000019879"},{"key":"bibr6-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240501205"},{"key":"bibr7-jhc.5A6733.2006","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1159\/000474164","volume":"30","author":"Bostwick DG","year":"1996","journal-title":"Eur Urol"},{"key":"bibr8-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0045(199608)29:2<117::AID-PROS7>3.0.CO;2-C"},{"key":"bibr9-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1042\/bj3260001"},{"key":"bibr10-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1159\/000012183"},{"key":"bibr11-jhc.5A6733.2006","first-page":"1924","volume":"10","author":"Downing SR","year":"2003","journal-title":"Can J Urol"},{"key":"bibr12-jhc.5A6733.2006","first-page":"31","volume":"4","author":"Feng P","year":"2000","journal-title":"Mol Urol"},{"key":"bibr13-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.10735"},{"key":"bibr14-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)66712-0"},{"key":"bibr15-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1988.tb05320.x"},{"key":"bibr16-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.1985.tb02607.x"},{"key":"bibr17-jhc.5A6733.2006","first-page":"69","volume-title":"Three-Dimensional Measurement in Microscopy","author":"Howard CV","year":"1998"},{"key":"bibr18-jhc.5A6733.2006","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1016\/S0022-3565(24)38207-2","volume":"289","author":"King LM","year":"1999","journal-title":"J Pharmacol Exp Ther"},{"key":"bibr19-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.5507\/bp.2003.001"},{"key":"bibr20-jhc.5A6733.2006","first-page":"191","volume":"154","author":"Marshall JR","year":"2001","journal-title":"IARC Sci Publ"},{"key":"bibr21-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0045(200101)46:1<11::AID-PROS1003>3.0.CO;2-K"},{"key":"bibr22-jhc.5A6733.2006","first-page":"185","volume":"21","author":"Martin R","year":"1999","journal-title":"Anal Quant Cytol Histol"},{"key":"bibr23-jhc.5A6733.2006","first-page":"238","volume":"19","author":"Montironi R","year":"1994","journal-title":"J Cell Biochem Suppl"},{"key":"bibr24-jhc.5A6733.2006","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1002\/j.1939-4640.2002.tb02241.x","volume":"23","author":"Morrissey C","year":"2002","journal-title":"J Androl"},{"key":"bibr25-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204504"},{"key":"bibr26-jhc.5A6733.2006","first-page":"543","volume":"3","author":"Nakamura K","year":"2002","journal-title":"Int J Oncol"},{"key":"bibr27-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1210\/endo.137.7.8770934"},{"key":"bibr28-jhc.5A6733.2006","first-page":"275","volume":"18","author":"Nicholson B","year":"2003","journal-title":"Histol Histopathol"},{"key":"bibr29-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1002\/pros.1061"},{"key":"bibr30-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1995.tb01520.x"},{"key":"bibr31-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0045(20001001)45:2<101::AID-PROS3>3.0.CO;2-P"},{"key":"bibr32-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(01)00673-5"},{"key":"bibr33-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.1070300409"},{"key":"bibr34-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1993.1193"},{"key":"bibr35-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.1993.tb02796.x"},{"key":"bibr36-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5742(00)00039-9"},{"key":"bibr37-jhc.5A6733.2006","first-page":"759","volume":"19","author":"Sirvent JJ","year":"2004","journal-title":"Histol Histopathol"},{"key":"bibr38-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1097\/00000372-199104000-00001"},{"key":"bibr39-jhc.5A6733.2006","first-page":"66","volume":"70","author":"Visser AJ","year":"1978","journal-title":"Trans Am Assoc Genitourin Surg"},{"key":"bibr40-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1016\/S0162-0134(00)00009-X"},{"key":"bibr41-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.3109\/10408449209145323"},{"key":"bibr42-jhc.5A6733.2006","first-page":"1227","volume":"61","author":"Winter RN","year":"2001","journal-title":"Cancer Res"},{"key":"bibr43-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.1070320505"},{"key":"bibr44-jhc.5A6733.2006","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2003.11.001"}],"container-title":["Journal of Histochemistry &amp; Cytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/jhc.5A6733.2006","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1369\/jhc.5A6733.2006","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/jhc.5A6733.2006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:26:54Z","timestamp":1777501614000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1369\/jhc.5A6733.2006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,9]]},"references-count":44,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2006,9]]}},"alternative-id":["10.1369\/jhc.5A6733.2006"],"URL":"https:\/\/doi.org\/10.1369\/jhc.5a6733.2006","relation":{},"ISSN":["0022-1554","1551-5044"],"issn-type":[{"value":"0022-1554","type":"print"},{"value":"1551-5044","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,9]]}}}