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In this work, we have produced monoclonal antibodies that are specific to human villous cytotrophoblast. Monoclonal antibody (MAb) MG2 was specific to villous cytotrophoblast across gestation, and recognizes hepatocyte growth factor activator inhibitor type 1. MAb MD10 stained villous cytotrophoblast across gestation and also some endothelial cells, particularly in the second or third trimester. MAb MD10 recognizes human integrin \u03b16\u03b24. As a test for specificity, the novel MAbs were also used for staining of frozen tissue from human colon carcinoma. The results show that the two antibodies can be used as tools to study human villous cytotrophoblasts and also human tumors. The MG2 antibody seems most specific and promising for the study of various aspects of human villous cytotrophoblast.<\/jats:p>","DOI":"10.1369\/jhc.5a6816.2006","type":"journal-article","created":{"date-parts":[[2006,2,22]],"date-time":"2006-02-22T20:53:59Z","timestamp":1140641639000},"page":"745-752","source":"Crossref","is-referenced-by-count":8,"title":["Identification of Antibodies against HAI-1 and Integrin \u03b16\u03b24 as Immunohistochemical Markers of Human Villous Cytotrophoblast"],"prefix":"10.1177","volume":"54","author":[{"given":"Outi K.","family":"Hallikas","sequence":"first","affiliation":[{"name":"Department of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johanna M.","family":"Aaltonen","sequence":"additional","affiliation":[{"name":"Minerva Medical Research Institute, Biomedicum Helsinki, Helsinki, 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