{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T17:55:17Z","timestamp":1778781317004,"version":"3.51.4"},"reference-count":14,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2007,10,1]],"date-time":"2007-10-01T00:00:00Z","timestamp":1191196800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:p>\n                    Pompe disease (glycogenosis type II) is a rare lysosomal disorder caused by a mutational deficiency of acid \u03b1-glucosidase (GAA). This deficiency leads to glycogen accumulation in multiple tissues: heart, skeletal muscles, and the central nervous system. A knockout mouse model mimicking the human condition has been used for histological evaluation. Currently, the best method for preserving glycogen in Pompe samples uses epon\u2013araldite resin. Although the preservation by this method is excellent, the size of the tissue is limited to 1 mm\n                    <jats:sup>3<\/jats:sup>\n                    . To accurately evaluate brain pathology in the Pompe mouse model, a modified glycol methacrylate (JB-4 Plus) method was developed. This approach allowed the production of larger tissue sections encompassing an entire mouse hemisphere (8 \u00d7 15 mm) while also providing a high level of morphological detail and preservation of glycogen. Application of the JB-4 Plus method is appropriate when a high level of cellular detail is desired. A modified paraffin method was also developed for use when rapid processing of multiple samples is a priority. Traditional paraffin processing results in glycogen loss. The modified paraffin method with periodic acid postfixation resulted in improved tissue morphology and glycogen preservation. Both techniques provide accurate anatomic evaluation of the glycogen distribution in Pompe mouse brain. (J Histochem Cytochem 55:991\u2013998, 2007)\n                  <\/jats:p>","DOI":"10.1369\/jhc.7a7239.2007","type":"journal-article","created":{"date-parts":[[2007,5,18]],"date-time":"2007-05-18T13:13:23Z","timestamp":1179494003000},"page":"991-998","source":"Crossref","is-referenced-by-count":14,"title":["Optimized Preservation of CNS Morphology for the Identification of Glycogen in the Pompe Mouse Model"],"prefix":"10.1177","volume":"55","author":[{"given":"Tatyana V.","family":"Taksir","sequence":"first","affiliation":[{"name":"Department of Pathology, Genzyme Corporation, Framingham, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise","family":"Griffiths","sequence":"additional","affiliation":[{"name":"Department of Pathology, Genzyme Corporation, Framingham, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Pathology, Genzyme Corporation, Framingham, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"Ryan","sequence":"additional","affiliation":[{"name":"Department of Pathology, Genzyme Corporation, Framingham, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lamya S.","family":"Shihabuddin","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Genzyme Corporation, Framingham, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beth L.","family":"Thurberg","sequence":"additional","affiliation":[{"name":"Department of Pathology, Genzyme Corporation, Framingham, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2007,10,1]]},"reference":[{"key":"bibr1-jhc.7A7239.2007","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199911)189:3<416::AID-PATH445>3.0.CO;2-6"},{"key":"bibr2-jhc.7A7239.2007","doi-asserted-by":"publisher","DOI":"10.1203\/01.pdr.0000233014.84318.4e"},{"key":"bibr3-jhc.7A7239.2007","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-197107000-00008"},{"key":"bibr4-jhc.7A7239.2007","first-page":"605","volume":"12","author":"Hall P","year":"1980","journal-title":"J Pathol"},{"key":"bibr5-jhc.7A7239.2007","doi-asserted-by":"publisher","DOI":"10.1179\/his.2000.23.1.51"},{"key":"bibr6-jhc.7A7239.2007","unstructured":"Luna LG (1968) Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, 3rd ed. New York, The Blakiston Division, McGraw-Hill Book Company, 3"},{"key":"bibr7-jhc.7A7239.2007","doi-asserted-by":"publisher","DOI":"10.1177\/002215540505300108"},{"key":"bibr8-jhc.7A7239.2007","unstructured":"McManus JFA, Mowry RW (1960) Staining Methods, Histological and Histochemical. New York, Hoeber, 126"},{"key":"bibr9-jhc.7A7239.2007","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.30.19086"},{"key":"bibr10-jhc.7A7239.2007","doi-asserted-by":"publisher","DOI":"10.3109\/10520296009114754"},{"key":"bibr11-jhc.7A7239.2007","first-page":"829","volume":"24","author":"Sakurai I","year":"1974","journal-title":"Acta Pathol Jpn"},{"key":"bibr12-jhc.7A7239.2007","volume-title":"Theory and Practice of Histo-technology","author":"Sheehan DC","year":"1980","edition":"2"},{"key":"bibr13-jhc.7A7239.2007","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700484"},{"key":"bibr14-jhc.7A7239.2007","doi-asserted-by":"publisher","DOI":"10.1177\/9.6.703"}],"container-title":["Journal of Histochemistry &amp; Cytochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/jhc.7A7239.2007","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1369\/jhc.7A7239.2007","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1369\/jhc.7A7239.2007","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:26:59Z","timestamp":1777501619000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1369\/jhc.7A7239.2007"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,10]]},"references-count":14,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["10.1369\/jhc.7A7239.2007"],"URL":"https:\/\/doi.org\/10.1369\/jhc.7a7239.2007","relation":{},"ISSN":["0022-1554","1551-5044"],"issn-type":[{"value":"0022-1554","type":"print"},{"value":"1551-5044","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,10]]}}}