{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T23:44:44Z","timestamp":1777851884120,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2015,2,19]],"date-time":"2015-02-19T00:00:00Z","timestamp":1424304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Histochem Cytochem."],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:p>MLL (mixed-lineage-leukemia) gene rearrangements are typical for acute leukemia and are associated with an aggressive course of disease, with a worse outcome than comparable case, and thus require intensified treatment. Here we describe a 69-year-old female with adult B cell precursor acute lymphoblastic leukemia (BCP-ALL) with hyperleukocytosis and immunophenotype CD10- and CD19+ with cryptic MLL rearrangements. G-banding at the time of diagnosis showed a normal karyotype: 46,XX. Molecular cytogenetics using multitude multicolor banding (mMCB) revealed a complex rearrangement of the two copies of chromosome 11. However, a locus-specific probe additionally identified that the MLL gene at 11q23.3 was disrupted, and that the 5\u2032 region was inserted into the chromosomal sub-band 4q21; thus the aberration involved three chromosomes and five break events. Unfortunately, the patient died six months after the initial diagnosis from serious infections and severe complications. Overall, the present findings confirm that, by far not all MLL aberrations are seen by routine chromosome banding techniques and that fluorescence in situ hybridization (FISH) should be regarded as standard tool to access MLL rearrangements in patients with BCP-ALL.<\/jats:p>","DOI":"10.1369\/0022155415576201","type":"journal-article","created":{"date-parts":[[2015,2,21]],"date-time":"2015-02-21T02:14:01Z","timestamp":1424484841000},"page":"384-390","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Novel Cryptic Rearrangements in Adult B-Cell Precursor Acute Lymphoblastic Leukemia Involving the MLL Gene"],"prefix":"10.1369","volume":"63","author":[{"given":"Moneeb A. K.","family":"Othman","sequence":"first","affiliation":[{"name":"Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Jena, Germany (MAKO, MR, KR, TL)"},{"name":"Cytogenetic Laboratory, Maria Sklodowska-Curie Memorial Cancer Centre and Institute, Warsaw, Poland (BG)"},{"name":"Department of Hematology, Institute of Hematology and Transfusion Medicine, Warsaw, Poland (BPG)"},{"name":"Laboratory of Cytogenetics and Genomics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal (JBM, IMC)"},{"name":"CIMAGO, Centro de Investiga\u00e7\u00e3o em Meio Ambiente, Gen\u00e9ticae Oncobiologia University of Coimbra, (JBM, IMC)"}]},{"given":"Beata","family":"Grygalewicz","sequence":"additional","affiliation":[{"name":"Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Jena, Germany (MAKO, MR, KR, TL)"},{"name":"Cytogenetic Laboratory, Maria Sklodowska-Curie Memorial Cancer Centre and Institute, Warsaw, Poland (BG)"},{"name":"Department of Hematology, Institute of Hematology and Transfusion Medicine, Warsaw, Poland (BPG)"},{"name":"Laboratory of Cytogenetics and Genomics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal (JBM, IMC)"},{"name":"CIMAGO, Centro de Investiga\u00e7\u00e3o em Meio Ambiente, Gen\u00e9ticae Oncobiologia University of Coimbra, (JBM, IMC)"}]},{"given":"Barbara","family":"Pienkowska-Grela","sequence":"additional","affiliation":[{"name":"Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Jena, Germany (MAKO, MR, KR, TL)"},{"name":"Cytogenetic Laboratory, Maria Sklodowska-Curie Memorial Cancer Centre and Institute, Warsaw, Poland (BG)"},{"name":"Department of Hematology, Institute of Hematology and Transfusion Medicine, Warsaw, Poland (BPG)"},{"name":"Laboratory of Cytogenetics and Genomics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal (JBM, IMC)"},{"name":"CIMAGO, Centro de Investiga\u00e7\u00e3o em Meio Ambiente, Gen\u00e9ticae Oncobiologia University of Coimbra, (JBM, IMC)"}]},{"given":"Martina","family":"Rincic","sequence":"additional","affiliation":[{"name":"Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Jena, Germany (MAKO, MR, KR, TL)"},{"name":"Cytogenetic Laboratory, Maria Sklodowska-Curie Memorial Cancer Centre and Institute, Warsaw, Poland (BG)"},{"name":"Department of Hematology, Institute of Hematology and Transfusion Medicine, Warsaw, Poland (BPG)"},{"name":"Laboratory of Cytogenetics and Genomics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal (JBM, IMC)"},{"name":"CIMAGO, Centro de Investiga\u00e7\u00e3o em Meio Ambiente, Gen\u00e9ticae Oncobiologia University of Coimbra, (JBM, IMC)"}]},{"given":"Katharina","family":"Rittscher","sequence":"additional","affiliation":[{"name":"Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Jena, Germany (MAKO, MR, KR, TL)"},{"name":"Cytogenetic Laboratory, Maria Sklodowska-Curie Memorial Cancer Centre and Institute, Warsaw, Poland (BG)"},{"name":"Department of Hematology, Institute of Hematology and Transfusion Medicine, Warsaw, Poland (BPG)"},{"name":"Laboratory of Cytogenetics and Genomics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal (JBM, IMC)"},{"name":"CIMAGO, Centro de Investiga\u00e7\u00e3o em Meio Ambiente, Gen\u00e9ticae Oncobiologia University of Coimbra, (JBM, IMC)"}]},{"given":"Joana B.","family":"Melo","sequence":"additional","affiliation":[{"name":"Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Jena, Germany (MAKO, MR, KR, TL)"},{"name":"Cytogenetic Laboratory, Maria Sklodowska-Curie Memorial Cancer Centre and Institute, Warsaw, Poland (BG)"},{"name":"Department of Hematology, Institute of Hematology and Transfusion Medicine, Warsaw, Poland (BPG)"},{"name":"Laboratory of Cytogenetics and Genomics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal (JBM, IMC)"},{"name":"CIMAGO, Centro de Investiga\u00e7\u00e3o em Meio Ambiente, Gen\u00e9ticae Oncobiologia University of Coimbra, (JBM, IMC)"}]},{"given":"Isabel M.","family":"Carreira","sequence":"additional","affiliation":[{"name":"Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Jena, Germany (MAKO, MR, KR, TL)"},{"name":"Cytogenetic Laboratory, Maria Sklodowska-Curie Memorial Cancer Centre and Institute, Warsaw, Poland (BG)"},{"name":"Department of Hematology, Institute of Hematology and Transfusion Medicine, Warsaw, Poland (BPG)"},{"name":"Laboratory of Cytogenetics and Genomics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal (JBM, IMC)"},{"name":"CIMAGO, Centro de Investiga\u00e7\u00e3o em Meio Ambiente, Gen\u00e9ticae Oncobiologia University of Coimbra, (JBM, IMC)"}]},{"given":"Britta","family":"Meyer","sequence":"additional","affiliation":[{"name":"Jena University Hospital, Friedrich Schiller University, Institute 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