{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:48:41Z","timestamp":1762271321346},"reference-count":79,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2009,4,3]],"date-time":"2009-04-03T00:00:00Z","timestamp":1238716800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.ploscompbiol.org"],"crossmark-restriction":false},"short-container-title":["PLoS Comput Biol"],"DOI":"10.1371\/journal.pcbi.1000344","type":"journal-article","created":{"date-parts":[[2009,4,2]],"date-time":"2009-04-02T22:07:42Z","timestamp":1238710062000},"page":"e1000344","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["Genome-Wide Functional Divergence after the Symbiosis of Proteobacteria with Insects Unraveled through a Novel Computational Approach"],"prefix":"10.1371","volume":"5","author":[{"given":"Christina","family":"Toft","sequence":"first","affiliation":[]},{"given":"Tom A.","family":"Williams","sequence":"additional","affiliation":[]},{"given":"Mario A.","family":"Fares","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2009,4,3]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1038\/385151a0","article-title":"Episodic adaptive evolution of primate lysozymes.","volume":"385","author":"W Messier","year":"1997","journal-title":"Nature"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1038\/366223a0","article-title":"Punctuated equilibrium comes of age.","volume":"366","author":"SJ Gould","year":"1993","journal-title":"Nature"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"14338","DOI":"10.1073\/pnas.0504070102","article-title":"Why highly expressed proteins evolve slowly.","volume":"102","author":"DA Drummond","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1534\/genetics.106.061754","article-title":"Thermodynamics of neutral protein evolution.","volume":"175","author":"JD Bloom","year":"2007","journal-title":"Genetics"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1093\/molbev\/msm019","article-title":"Proportion of solvent-exposed amino acids in a protein and rate of protein evolution.","volume":"24","author":"YS Lin","year":"2007","journal-title":"Mol Biol Evol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/S0968-0004(02)02094-7","article-title":"Predicting functional divergence in protein evolution by site-specific rate shifts.","volume":"27","author":"EA Gaucher","year":"2002","journal-title":"Trends Biochem Sci"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/oxfordjournals.molbev.a003973","article-title":"Heterotachy, an important process of protein evolution.","volume":"19","author":"P Lopez","year":"2002","journal-title":"Mol Biol Evol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1002\/prot.20550","article-title":"Large-scale prediction of function shift in protein families with a focus on enzymatic function.","volume":"60","author":"S Abhiman","year":"2005","journal-title":"Proteins"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1002\/jez.b.21120","article-title":"Genome evolution and functional divergence in Yersinia.","volume":"308","author":"J Gu","year":"2007","journal-title":"J Exp Zool B Mol Dev Evol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1007\/BF00486096","article-title":"An improved method for determining codon variability in a gene and its application to the rate of fixation of mutations in evolution.","volume":"4","author":"WM Fitch","year":"1970","journal-title":"Biochem Genet"},{"key":"ref11","article-title":"Evolution by Gene Duplication","author":"S Ohno","year":"1970"},{"key":"ref12","first-page":"94","article-title":"Rapid evolution of goat and sheep globin genes following gene duplication.","volume":"1","author":"WH Li","year":"1983","journal-title":"Mol Biol Evol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"2950","DOI":"10.1073\/pnas.91.8.2950","article-title":"Invasion and maintenance of a gene duplication.","volume":"91","author":"AG Clark","year":"1994","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1098\/rspb.1994.0058","article-title":"The evolution of functionally novel proteins after gene duplication.","volume":"256","author":"AL Hughes","year":"1994","journal-title":"Proc Biol Sci"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/S0168-9525(96)80020-5","article-title":"The coevolution of gene family trees.","volume":"12","author":"KJ Fryxell","year":"1996","journal-title":"Trends Genet"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"7799","DOI":"10.1073\/pnas.94.15.7799","article-title":"Evolution by the birth-and-death process in multigene families of the vertebrate immune system.","volume":"94","author":"M Nei","year":"1997","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1093\/genetics\/151.4.1531","article-title":"Preservation of duplicate genes by complementary, degenerative mutations.","volume":"151","author":"A Force","year":"1999","journal-title":"Genetics"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/S0168-9525(03)00139-2","article-title":"Evolution of duplicate genes versus genetic robustness against null mutations.","volume":"19","author":"X Gu","year":"2003","journal-title":"Trends Genet"},{"key":"ref19","article-title":"The ecology of adaptive radiation","author":"D Schluter","year":"2000"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1111\/j.1461-0248.2004.00715.x","article-title":"Ecological speciation.","volume":"8","author":"HD Rundle","year":"2005","journal-title":"Ecol Lett"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"402","DOI":"10.2307\/2446333","article-title":"Phylogeny and radiation of pollination systems in DISA (Orchidaceae).","volume":"85","author":"SD Johnson","year":"1998","journal-title":"Am J Bot"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1600\/036364406777585928","article-title":"Flowering phenology in relation to adaptive radiation.","volume":"31","author":"DA Levin","year":"2006","journal-title":"Syst Bot"},{"key":"ref23","first-page":"262","article-title":"The web of life: development of over 3.8 billion years of trophic relationships.","author":"PW Price","year":"1991"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S0966-842X(98)01312-2","article-title":"Reductive evolution of resident genomes.","volume":"6","author":"SG Andersson","year":"1998","journal-title":"Trends Microbiol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1126\/science.1130441","article-title":"A small microbial genome: the end of a long symbiotic relationship?","volume":"314","author":"V Perez-Brocal","year":"2006","journal-title":"Science"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"2069","DOI":"10.1093\/molbev\/msn153","article-title":"The evolution of the flagellar assembly pathway in endosymbiotic bacterial genomes.","volume":"25","author":"C Toft","year":"2008","journal-title":"Mol Biol Evol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"2873","DOI":"10.1073\/pnas.93.7.2873","article-title":"Accelerated evolution and Muller's rachet in endosymbiotic bacteria.","volume":"93","author":"NA Moran","year":"1996","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1093\/oxfordjournals.molbev.a025834","article-title":"Mutation accumulation in nuclear, organelle, and prokaryotic transfer RNA genes.","volume":"14","author":"M Lynch","year":"1997","journal-title":"Mol Biol Evol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1093\/oxfordjournals.molbev.a025557","article-title":"Mutation accumulation in transfer RNAs: molecular evidence for Muller's ratchet in mitochondrial genomes.","volume":"13","author":"M Lynch","year":"1996","journal-title":"Mol Biol Evol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1086\/303396","article-title":"Accumulation of deleterious mutations in endosymbionts: Muller's ratchet with two levels of selection.","volume":"156","author":"C Rispe","year":"2000","journal-title":"Am Nat"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1093\/oxfordjournals.molbev.a025958","article-title":"Evolutionary rates for tuf genes in endosymbionts of aphids.","volume":"15","author":"EU Brynnel","year":"1998","journal-title":"Mol Biol Evol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.1093\/oxfordjournals.molbev.a026071","article-title":"Sequence evolution in bacterial endosymbionts having extreme base compositions.","volume":"16","author":"MA Clark","year":"1999","journal-title":"Mol Biol Evol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1093\/genetics\/157.2.477","article-title":"Intraspecific variation in symbiont genomes: bottlenecks and the aphid-buchnera association.","volume":"157","author":"DJ Funk","year":"2001","journal-title":"Genetics"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1098\/rspb.2000.1159","article-title":"Decay of mutualistic potential in aphid endosymbionts through silencing of biosynthetic loci: Buchnera of Diuraphis.","volume":"267","author":"JJ Wernegreen","year":"2000","journal-title":"Proc Biol Sci"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"4454","DOI":"10.1073\/pnas.062067299","article-title":"Extreme genome reduction in Buchnera spp.: toward the minimal genome needed for symbiotic life.","volume":"99","author":"R Gil","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"2649","DOI":"10.1046\/j.1365-294X.2002.01646.x","article-title":"Extremely low levels of genetic polymorphism in endosymbionts (Buchnera) of aphids (Pemphigus).","volume":"11","author":"P Abbot","year":"2002","journal-title":"Mol Ecol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"4458","DOI":"10.1073\/pnas.95.8.4458","article-title":"Deleterious mutations destabilize ribosomal RNA in endosymbiotic bacteria.","volume":"95","author":"JD Lambert","year":"1998","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1073\/pnas.0235981100","article-title":"Reductive genome evolution in Buchnera aphidicola.","volume":"100","author":"RC van Ham","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"6539","DOI":"10.1128\/JB.00561-06","article-title":"Hundreds of flagellar basal bodies cover the cell surface of the endosymbiotic bacterium Buchnera aphidicola sp. strain APS.","volume":"188","author":"K Maezawa","year":"2006","journal-title":"J Bacteriol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1038\/35024074","article-title":"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS.","volume":"407","author":"S Shigenobu","year":"2000","journal-title":"Nature"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1093\/molbev\/msi036","article-title":"Nonhomogeneous model of sequence evolution indicates independent origins of primary endosymbionts within the enterobacteriales (gamma-Proteobacteria).","volume":"22","author":"JT Herbeck","year":"2005","journal-title":"Mol Biol Evol"},{"key":"ref42","first-page":"811","article-title":"Endosymbionts of insects.","author":"GA Dasch","year":"1984"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1111\/j.1365-2958.1996.tb02557.x","article-title":"Intracellular endosymbiotic bacteria of Camponotus species (carpenter ants): systematics, evolution and ultrastructural characterization.","volume":"21","author":"D Schroder","year":"1996","journal-title":"Mol Microbiol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"993","DOI":"10.2108\/zsj.16.993","article-title":"Phylogenetic comparison of endosymbionts with their host ants based on molecular evidence.","volume":"16","author":"S Sameshima","year":"1999","journal-title":"Zool Sci"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/PL00001690","article-title":"Contributions to the life history of the Malaysian giant ant Camponotus gigas (Hymenoptera, Formicidae).","volume":"47","author":"M Pfeiffer","year":"2000","journal-title":"Insectes Sociaux"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"6027","DOI":"10.1128\/AEM.00933-06","article-title":"Relevance of the endosymbiosis of Blochmannia floridanus and carpenter ants at different stages of the life cycle of the host.","volume":"72","author":"E Zientz","year":"2006","journal-title":"Appl Environ Microbiol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1038\/sj.hdy.6801031","article-title":"Looking for Darwin in all the wrong places: the misguided quest for positive selection at the nucleotide sequence level.","volume":"99","author":"AL Hughes","year":"2007","journal-title":"Heredity"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"1877","DOI":"10.1099\/00207713-50-5-1877","article-title":"Systematic relationships and cospeciation of bacterial endosymbionts and their carpenter ant host species: proposal of the new taxon Candidatus Blochmannia gen. nov.","volume":"50","author":"C Sauer","year":"2000","journal-title":"Int J Syst Evol Microbiol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1146\/annurev.ento.43.1.17","article-title":"Nutritional interactions in insect-microbial symbioses: aphids and their symbiotic bacteria Buchnera.","volume":"43","author":"AE Douglas","year":"1998","journal-title":"Annu Rev Entomol"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/S0022-1910(99)00098-0","article-title":"Nutritional enhancement of host plants by aphids - a comparison of three aphid species on grasses.","volume":"46","author":"J Sandstrom","year":"2000","journal-title":"J Insect Physiol"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1093\/oxfordjournals.molbev.a004174","article-title":"The evolution of the heat-shock protein GroEL from Buchnera, the primary endosymbiont of aphids, is governed by positive selection.","volume":"19","author":"MA Fares","year":"2002","journal-title":"Mol Biol Evol"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1038\/417398a","article-title":"Endosymbiotic bacteria: groEL buffers against deleterious mutations.","volume":"417","author":"MA Fares","year":"2002","journal-title":"Nature"},{"key":"ref53","article-title":"Morphological and molecular studies on intracellular bacterial symbiotes of insects","author":"GA Dasch","year":"1975"},{"key":"ref54","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-662-10306-7","article-title":"The Ants","author":"B Holldobler","year":"1990"},{"key":"ref55","article-title":"Identification Guide to the Ant Genera of the World","author":"B Bolton","year":"1994"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1111\/j.1095-8312.1997.tb01785.x","article-title":"The role of resource imbalances in the evolutionary ecology of tropical arboreal ants.","volume":"61","author":"DW Davidson","year":"1997","journal-title":"Biological Journal of the Linnean Society"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1046\/j.1365-2311.1998.00145.x","article-title":"Resource discovery versus resource domination in ants: a functional mechanism for breaking the trade-off.","volume":"23","author":"DW Davidson","year":"1998","journal-title":"Ecol Entomol"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"1664","DOI":"10.1093\/oxfordjournals.molbev.a026080","article-title":"Statistical methods for testing functional divergence after gene duplication.","volume":"16","author":"X Gu","year":"1999","journal-title":"Mol Biol Evol"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1093\/oxfordjournals.molbev.a003824","article-title":"Maximum-likelihood approach for gene family evolution under functional divergence.","volume":"18","author":"X Gu","year":"2001","journal-title":"Mol Biol Evol"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.1093\/molbev\/msl056","article-title":"A simple statistical method for estimating type-II (cluster-specific) functional divergence of protein sequences.","volume":"23","author":"X Gu","year":"2006","journal-title":"Mol Biol Evol"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1007\/PL00006572","article-title":"The root of the tree of life in the light of the covarion model.","volume":"49","author":"P Lopez","year":"1999","journal-title":"J Mol Evol"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1186\/1471-2105-6-137","article-title":"SplitTester: software to identify domains responsible for functional divergence in protein family.","volume":"6","author":"X Gao","year":"2005","journal-title":"BMC Bioinformatics"},{"key":"ref63","doi-asserted-by":"crossref","first-page":"9388","DOI":"10.1073\/pnas.1533499100","article-title":"The genome sequence of Blochmannia floridanus: comparative analysis of reduced genomes.","volume":"100","author":"R Gil","year":"2003","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/1741-7007-5-48","article-title":"Nutritional upgrading for omnivorous carpenter ants by the endosymbiont Blochmannia.","volume":"5","author":"H Feldhaar","year":"2007","journal-title":"BMC Biol"},{"key":"ref65","doi-asserted-by":"crossref","first-page":"12221","DOI":"10.1073\/pnas.211106398","article-title":"Microinjection and growth of bacteria in the cytosol of mammalian host cells.","volume":"98","author":"M Goetz","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref66","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","article-title":"CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.","volume":"22","author":"JD Thompson","year":"1994","journal-title":"Nucleic Acids Res"},{"key":"ref67","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1016\/S0959-437X(02)00348-9","article-title":"Inference of selection from multiple species alignments.","volume":"12","author":"Z Yang","year":"2002","journal-title":"Curr Opin Genet Dev"},{"key":"ref68","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1093\/molbev\/msh084","article-title":"Evidence of positively selected sites in mammalian alpha-defensins.","volume":"21","author":"DJ Lynn","year":"2004","journal-title":"Mol Biol Evol"},{"key":"ref69","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1038\/nrg1770","article-title":"Hearing silence: non-neutral evolution at synonymous sites in mammals.","volume":"7","author":"JV Chamary","year":"2006","journal-title":"Nat Rev Genet"},{"key":"ref70","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1093\/molbev\/msj035","article-title":"Evidence for purifying selection against synonymous mutations in mammalian exonic splicing enhancers.","volume":"23","author":"JL Parmley","year":"2006","journal-title":"Mol Biol Evol"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"i319","DOI":"10.1093\/bioinformatics\/btm176","article-title":"Towards realistic codon models: among site variability and dependency of synonymous and non-synonymous rates.","volume":"23","author":"I Mayrose","year":"2007","journal-title":"Bioinformatics"},{"key":"ref72","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1093\/molbev\/msm100","article-title":"Widespread positive selection in synonymous sites of mammalian genes.","volume":"24","author":"AM Resch","year":"2007","journal-title":"Mol Biol Evol"},{"key":"ref73","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.1093\/molbev\/msm088","article-title":"PAML 4: phylogenetic analysis by maximum likelihood.","volume":"24","author":"Z Yang","year":"2007","journal-title":"Mol Biol Evol"},{"key":"ref74","first-page":"418","article-title":"Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions.","volume":"3","author":"M Nei","year":"1986","journal-title":"Mol Biol Evol"},{"key":"ref75","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1007\/BF02111283","article-title":"Evolution in bacteria: evidence for a universal substitution rate in cellular genomes.","volume":"26","author":"H Ochman","year":"1987","journal-title":"J Mol Evol"},{"key":"ref76","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1101\/gr.1358104","article-title":"Mutational and selective pressures on codon and amino acid usage in Buchnera, endosymbiotic bacteria of aphids.","volume":"14","author":"C Rispe","year":"2004","journal-title":"Genome Res"},{"key":"ref77","article-title":"Selection for translational robustness in Buchnera aphidicola, endosymbiotic bacteria of aphids.","author":"C Toft","year":"2009","journal-title":"Mol Biol Evol"},{"key":"ref78","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: kyoto encyclopedia of genes and genomes.","volume":"28","author":"M Kanehisa","year":"2000","journal-title":"Nucleic Acids Res"},{"key":"ref79","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1126\/science.278.5338.631","article-title":"A genomic perspective on protein families.","volume":"278","author":"RL Tatusov","year":"1997","journal-title":"Science"}],"container-title":["PLoS Computational Biology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pcbi.1000344","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,3]],"date-time":"2021-10-03T23:08:58Z","timestamp":1633302538000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pcbi.1000344"}},"subtitle":[],"editor":[{"given":"Csaba","family":"P\u00e1l","sequence":"first","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2009,4,3]]},"references-count":79,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2009,4,3]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pcbi.1000344","relation":{},"ISSN":["1553-7358"],"issn-type":[{"value":"1553-7358","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,4,3]]}}}