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Stockholm: Internal Report SUF\u2013PFY\/96\u201301; 2007."},{"key":"ref34","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1080\/00949658008810370","article-title":"Monte carlo estimates of the distributions of the random polygons of the voronoi tessellation with respect to a poisson process","volume":"10","author":"AL Hinde","year":"1980","journal-title":"J Stat Comput Simul"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1093\/biomet\/asq047","article-title":"On the Voronoi estimator for the intensity of an inhomogeneous planar Poisson process","volume":"97","author":"CD Barr","year":"2010","journal-title":"Biometrika"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"10062","DOI":"10.1016\/S0021-9258(17)44606-0","article-title":"Coating cells with colloidal silica for high yield isolation of plasma membrane sheets and identification of transmembrane proteins","volume":"258","author":"LK Chaney","year":"1983","journal-title":"J Biol 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