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L. McDonald","year":"2006","journal-title":"Tech Insight High-Pressure Freezing, Cellular Tomography, and Structural Cell Biology Sample Preservation for Biological Electron"},{"issue":"no. 3","key":"ref2","first-page":"343","article-title":"Dual-axis tomography: an approach with alignment methods that preserve resolution","volume":"vol. 120","author":"D. N. Mastronarde","year":"1997"},{"issue":"no. 12","key":"ref3","first-page":"4388","article-title":"The presynaptic dense projection of the Caenorhabditis elegans cholinergic neuromuscular junction localizes synaptic vesicles at the active zone through SYD-2\/liprin and UNC-10\/RIM-dependent interactions","volume":"vol. 31","author":"C. Stigloher","year":"2011"},{"issue":"no. 16","key":"ref4","article-title":"Conical Electron Tomography of a Chemical Synapse: Polyhedral Cages Dock Vesicles to the Active Zone","volume":"vol. 28","author":"G. A. 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Schulze","year":"2013"},{"issue":"no. 5","key":"ref10","first-page":"1096","article-title":"Learning Structured Models for Segmentation of 2-D and 3-D Imagery","volume":"vol. 34","author":"A. Lucchi","year":"2015"},{"key":"ref11","first-page":"49","article-title":"Robust membrane detection based on tensor voting for electron tomography","volume":"vol. 186","author":"A. Martinez-Sanchez","year":"2014"},{"issue":"no. 1","key":"ref12","first-page":"135","article-title":"Automated segmentation of electron tomograms for a quantitative description of actin filament networks","volume":"vol. 177","author":"A. Rigort","year":"2012"},{"issue":"no. 3","key":"ref13","first-page":"e33697","article-title":"A 3D image filter for parameter-free segmentation of macromolecular structures from electron tomograms","volume":"vol. 7","author":"R. A. 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Helmprobst","year":"2015"},{"key":"ref23","first-page":"2532","article-title":"Septins promote dendrite and axon development by negatively regulating microtubule stability via HDAC6-mediated deacetylation","volume":"vol. 4","author":"N. Ageta-Ishihara","year":"2013"},{"issue":"no. 2","key":"ref24","first-page":"148","article-title":"Daughter cell identity emerges from the interplay of Cdc42, septins, and exocytosis","volume":"vol. 26","author":"S. Okada","year":"2013"},{"key":"ref25","doi-asserted-by":"crossref","DOI":"10.1074\/jbc.M114.616201","article-title":"Septin dynamics are essential for exocytosis","author":"E. Tokhtaeva","year":"2015"},{"issue":"no. 1","key":"ref26","first-page":"16","article-title":"Synaptic dysfunction and septin protein family members in neurodegenerative diseases","volume":"vol. 10","author":"M. Marttinen","year":"2015"},{"issue":"no. 2","key":"ref27","first-page":"123","article-title":"Septin functions in organ system physiology and pathology","volume":"vol. 395","author":"L. Dolat","year":"2014"},{"issue":"no. 8\u20139","key":"ref28","first-page":"763","article-title":"Septin genomics: a road less travelled","volume":"vol. 392","author":"S. E. H. Russell","year":"2011"},{"issue":"no. 3","key":"ref29","first-page":"423","article-title":"Three-dimensional ultrastructure of the septin filament network in Saccharomyces cerevisiae","volume":"vol. 23","author":"A. Bertin","year":"2012"},{"issue":"no. 8","key":"ref30","first-page":"414","article-title":"Some assembly required: yeast septins provide the instruction manual","volume":"vol. 15","author":"M. Versele","year":"2005"},{"issue":"no. 11","key":"ref31","first-page":"236","article-title":"The septins","volume":"vol. 4","author":"M. Kinoshita","year":"2003"},{"key":"ref32","first-page":"71","article-title":"The Genetics of Caenorhabditis elegans","author":"S. Brenner","year":"1973","journal-title":"The Genetics of Caenorhabditis elegans"},{"issue":"no. 1","key":"ref33","first-page":"220","article-title":"A role for septins in cellular and axonal migration in C. elegans","volume":"vol. 261","author":"F. P. Finger","year":"2003"},{"issue":"no. 1","key":"ref34","first-page":"41","article-title":"Abnormal cell lineages in mutants of the nematode Caenorhabditis elegans","volume":"vol. 82","author":"J. E. Sulston","year":"1981"},{"issue":"no. 5867","key":"ref35","first-page":"584","article-title":"Neurone differentiation in cell lineage mutants of Caenorhabditis elegans","volume":"vol. 297","author":"J. G. White","year":"1982"},{"issue":"Pt 21","key":"ref36","first-page":"3825","article-title":"The C. elegans septin genes, unc-59 and unc-61, are required for normal postembryonic cytokineses and morphogenesis but have no essential function in embryogenesis","volume":"vol. 113","author":"T. Q. Nguyen","year":"2000"},{"issue":"no. 14","key":"ref37","first-page":"1840","article-title":"TANGO: a generic tool for high-throughput 3D image analysis for studying nuclear organization","volume":"vol. 29","author":"J. Ollion","year":"2013"},{"issue":"no. 1","key":"ref38","first-page":"274","article-title":"A high-level 3D visualization API for Java and ImageJ","volume":"vol. 11","author":"B. Schmid","year":"2010"},{"issue":"no. 1","key":"ref39","first-page":"71","article-title":"Computer visualization of three-dimensional image data using IMOD","volume":"vol. 116","author":"J. R. Kremer","year":"1996"},{"key":"ref40","first-page":"368","article-title":"Segmentation of electron tomographic data sets using fuzzy set theory principles","volume":"vol. 162","author":"E. Gardun","year":"2008"},{"issue":"no. 14","key":"ref41","first-page":"3296","article-title":"The Caenorhabditis elegans septin complex is nonpolar","volume":"vol. 26","author":"C. M. John","year":"2007"},{"issue":"no. 5","key":"ref42","first-page":"434","article-title":"The septin CDCrel-1 binds syntaxin and inhibits exocytosis","volume":"vol. 2","author":"C. L. Beites","year":"1999"},{"issue":"no. 10","key":"ref43","first-page":"1864","article-title":"Learning Context Cues for Synapse Segmentation","volume":"vol. 32","author":"C. Becker","year":"2013"},{"key":"ref44","doi-asserted-by":"crossref","unstructured":"R. Kumar, A. Vazquez-Reina, and H. 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