{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T16:00:56Z","timestamp":1777737656629,"version":"3.51.4"},"update-to":[{"DOI":"10.1371\/journal.pcbi.1005612","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2017,7,7]],"date-time":"2017-07-07T00:00:00Z","timestamp":1499385600000}}],"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T00:00:00Z","timestamp":1498089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["435605-2013"],"award-info":[{"award-number":["435605-2013"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fondation de l'UQTR"}],"content-domain":{"domain":["www.ploscompbiol.org"],"crossmark-restriction":false},"short-container-title":["PLoS Comput Biol"],"DOI":"10.1371\/journal.pcbi.1005612","type":"journal-article","created":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T13:27:45Z","timestamp":1498138065000},"page":"e1005612","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":50,"title":["A novel algorithm identifies stress-induced alterations in mitochondrial connectivity and inner membrane structure from confocal images"],"prefix":"10.1371","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0651-4817","authenticated-orcid":true,"given":"Mathieu","family":"Ouellet","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00e9rald","family":"Guillebaud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valerie","family":"Gervais","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Lupien St-Pierre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7942-3185","authenticated-orcid":true,"given":"Marc","family":"Germain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2017,6,22]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nature12985","article-title":"Mitochondrial form and function","volume":"505","author":"JR Friedman","year":"2014","journal-title":"Nature"},{"key":"ref2","article-title":"Mitochondria: from cell death executioners to regulators of cell differentiation","author":"A Kasahara","year":"2014","journal-title":"Trends Cell Biol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1016\/j.cell.2012.02.035","article-title":"Mitochondria: in sickness and in health","volume":"148","author":"J Nunnari","year":"2012","journal-title":"Cell"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s00401-011-0930-z","article-title":"Less than perfect divorces: dysregulated mitochondrial fission and neurodegeneration","volume":"123","author":"B Oettinghaus","year":"2012","journal-title":"Acta Neuropathol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.molmed.2014.01.005","article-title":"Mitochondrial pathology: stress signals from the energy factory","volume":"20","author":"N Raimundo","year":"2014","journal-title":"Trends Mol Med"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1546","DOI":"10.1038\/sj.emboj.7600592","article-title":"Endoplasmic reticulum BIK initiates DRP1-regulated remodelling of mitochondrial cristae during apoptosis","volume":"24","author":"M Germain","year":"2005","journal-title":"Embo J"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/S1534-5807(01)00116-2","article-title":"A distinct pathway remodels mitochondrial cristae and mobilizes cytochrome c during apoptosis","volume":"2","author":"L Scorrano","year":"2002","journal-title":"Dev Cell"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1038\/ncb2220","article-title":"During autophagy mitochondria elongate, are spared from degradation and sustain cell viability","volume":"13","author":"LC Gomes","year":"2011","journal-title":"Nat Cell Biol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"3550","DOI":"10.1038\/ncomms4550","article-title":"Acidosis overrides oxygen deprivation to maintain mitochondrial function and cell survival","volume":"5","author":"M Khacho","year":"2014","journal-title":"Nat Commun"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"2676","DOI":"10.15252\/embj.201488349","article-title":"OPA1-dependent cristae modulation is essential for cellular adaptation to metabolic demand","volume":"33","author":"DA Patten","year":"2014","journal-title":"EMBO J"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"10190","DOI":"10.1073\/pnas.1107402108","article-title":"Tubular network formation protects mitochondria from autophagosomal degradation during nutrient starvation","volume":"108","author":"AS Rambold","year":"2011","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1038\/emboj.2009.89","article-title":"SLP-2 is required for stress-induced mitochondrial hyperfusion","volume":"28","author":"D Tondera","year":"2009","journal-title":"EMBO J"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"3038","DOI":"10.1038\/emboj.2012.170","article-title":"Mitochondrial dysfunction in Parkinson&apos;s disease: molecular mechanisms and pathophysiological consequences","volume":"31","author":"N Exner","year":"2012","journal-title":"Embo J"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"e461","DOI":"10.1038\/cddis.2012.213","article-title":"Computational classification of mitochondrial shapes reflects stress and redox state","volume":"4","author":"T Ahmad","year":"2013","journal-title":"Cell Death Dis"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1073\/pnas.1311310110","article-title":"Presynaptic mitochondrial morphology in monkey prefrontal cortex correlates with working memory and is improved with estrogen treatment","volume":"111","author":"Y Hara","year":"2014","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.bbamcr.2014.11.002","article-title":"Quantitative analysis of mitochondrial morphology and membrane potential in living cells using high-content imaging, machine learning, and morphological binning","volume":"1853","author":"AP Leonard","year":"2015","journal-title":"Biochim Biophys Acta"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1038\/cdd.2011.13","article-title":"Altered fusion dynamics underlie unique morphological changes in mitochondria during hypoxia-reoxygenation stress","volume":"18","author":"X Liu","year":"2011","journal-title":"Cell Death Differ"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"e1002212","DOI":"10.1371\/journal.pcbi.1002212","article-title":"Automatic morphological subtyping reveals new roles of caspases in mitochondrial dynamics","volume":"7","author":"JY Peng","year":"2011","journal-title":"PLoS Comput Biol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"e28694","DOI":"10.1371\/journal.pone.0028694","article-title":"Multi-parametric analysis and modeling of relationships between mitochondrial morphology and apoptosis","volume":"7","author":"Y Reis","year":"2012","journal-title":"PLoS One"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1016\/j.biocel.2012.03.006","article-title":"Standardized mitochondrial analysis gives new insights into mitochondrial dynamics and OPA1 function","volume":"44","author":"A Chevrollier","year":"2012","journal-title":"Int J Biochem Cell Biol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/cyto.a.20198","article-title":"Simultaneous quantitative measurement and automated analysis of mitochondrial morphology, mass, potential, and motility in living human skin fibroblasts","volume":"69","author":"WJ Koopman","year":"2006","journal-title":"Cytometry A"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"e101365","DOI":"10.1371\/journal.pone.0101365","article-title":"Automated quantification and integrative analysis of 2D and 3D mitochondrial shape and network properties","volume":"9","author":"J Nikolaisen","year":"2014","journal-title":"PLoS One"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1093\/bioinformatics\/bts073","article-title":"Mytoe: automatic analysis of mitochondrial dynamics","volume":"28","author":"E Lihavainen","year":"2012","journal-title":"Bioinformatics"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.jneumeth.2016.01.008","article-title":"Quantification of mitochondrial morphology in neurites of dopaminergic neurons using multiple parameters","volume":"262","author":"L Wiemerslage","year":"2016","journal-title":"J Neurosci Methods"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"4772","DOI":"10.1074\/jbc.M110.167155","article-title":"The mitochondrial inner membrane GTPase, optic atrophy 1 (Opa1), restores mitochondrial morphology and promotes neuronal survival following excitotoxicity","volume":"286","author":"A Jahani-Asl","year":"2011","journal-title":"J Biol Chem"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"Levina E, Bickel P (2001) The Earth Mover&apos;s distance is the Mallows distance: Some insights from statistics. Proceedings Eighth IEEE International Conference on Computer Vision: IEEE. pp. 251\u2013256.","DOI":"10.1109\/ICCV.2001.937632"},{"key":"ref27","doi-asserted-by":"crossref","unstructured":"Rubner Y, Tomasi C, Guibas LJ (1998) A metric for distributions with applications to image databases. Proceedings of the IEEE International Conference on Computer Vision. Bombay, India. pp. 59\u201366.","DOI":"10.1109\/ICCV.1998.710701"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1023\/A:1026543900054","article-title":") The Earth Mover\u2019s Distance as a Metric for Image Retrieval","volume":"40","author":"Y Rubner","year":"2000","journal-title":"Int J Comput Vis"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1016\/j.devcel.2015.01.029","article-title":"Fatty acid trafficking in starved cells: regulation by lipid droplet lipolysis, autophagy, and mitochondrial fusion dynamics","volume":"32","author":"AS Rambold","year":"2015","journal-title":"Dev Cell"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1126\/science.aab4138","article-title":"AMP-activated protein kinase mediates mitochondrial fission in response to energy stress","volume":"351","author":"EQ Toyama","year":"2016","journal-title":"Science"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.cell.2013.08.032","article-title":"Mitochondrial Cristae Shape Determines Respiratory Chain Supercomplexes Assembly and Respiratory Efficiency","volume":"155","author":"S Cogliati","year":"2013","journal-title":"Cell"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"21649","DOI":"10.1074\/jbc.M803236200","article-title":"The mechanism of mitochondrial superoxide production by the cytochrome bc1 complex","volume":"283","author":"S Drose","year":"2008","journal-title":"J Biol Chem"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"10263","DOI":"10.1074\/jbc.M115.695825","article-title":"Loss of Mitochondrial Function Impairs Lysosomes","volume":"291","author":"J Demers-Lamarche","year":"2016","journal-title":"J Biol Chem"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1543","DOI":"10.1091\/mbc.E04-08-0697","article-title":"The mitochondrial inner membrane protein mitofilin controls cristae morphology","volume":"16","author":"GB John","year":"2005","journal-title":"Mol Biol Cell"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1016\/S1097-2765(00)80391-3","article-title":"C. elegans dynamin-related protein DRP-1 controls severing of the mitochondrial outer membrane","volume":"4","author":"AM Labrousse","year":"1999","journal-title":"Mol Cell"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"5635","DOI":"10.1038\/sj.emboj.7601438","article-title":"Cytosolic factor- and TOM-independent import of C-tail-anchored mitochondrial outer membrane proteins","volume":"25","author":"K Setoguchi","year":"2006","journal-title":"EMBO J"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1093\/hmg\/dds500","article-title":"LKB1-regulated adaptive mechanisms are essential for neuronal survival following mitochondrial dysfunction","volume":"22","author":"M Germain","year":"2013","journal-title":"Hum Mol Genet"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1083\/jcb.77.1.134","article-title":"A study of rapid mitochondrial structural changes in vitro by spray-freeze-etching","volume":"77","author":"RD Lang","year":"1978","journal-title":"J Cell Biol"},{"key":"ref39","doi-asserted-by":"crossref","unstructured":"Harner ME, Unger AK, Geerts WJ, Mari M, Izawa T, et al. (2016) An evidence based hypothesis on the existence of two pathways of mitochondrial crista formation. Elife 5.","DOI":"10.7554\/eLife.18853"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.mito.2015.12.003","article-title":"Fully automated software for quantitative measurements of mitochondrial morphology","volume":"26","author":"PM McClatchey","year":"2016","journal-title":"Mitochondrion"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/S1534-5807(01)00055-7","article-title":"The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis","volume":"1","author":"S Frank","year":"2001","journal-title":"Dev Cell"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.molcel.2004.09.026","article-title":"Drp-1-dependent division of the mitochondrial network blocks intraorganellar Ca2+ waves and protects against Ca2+-mediated apoptosis","volume":"16","author":"G Szabadkai","year":"2004","journal-title":"Mol Cell"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.tibs.2016.01.001","article-title":"Mitochondrial Cristae: Where Beauty Meets Functionality","volume":"41","author":"S Cogliati","year":"2016","journal-title":"Trends Biochem Sci"},{"key":"ref44","article-title":"Role of the mitochondrial contact site and cristae organizing system in membrane architecture and dynamics","author":"H Rampelt","year":"2016","journal-title":"Biochim Biophys Acta"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1515\/hsz-2012-0239","article-title":"Mitofilin complexes: conserved organizers of mitochondrial membrane architecture","volume":"393","author":"RM Zerbes","year":"2012","journal-title":"Biol Chem"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1016\/j.tibs.2013.09.001","article-title":"Unearthing the secrets of mitochondrial ROS and glutathione in bioenergetics","volume":"38","author":"RJ Mailloux","year":"2013","journal-title":"Trends Biochem Sci"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"e1002745","DOI":"10.1371\/journal.pcbi.1002745","article-title":"Emergence of the mitochondrial reticulum from fission and fusion dynamics","volume":"8","author":"VM Sukhorukov","year":"2012","journal-title":"PLoS Comput Biol"}],"updated-by":[{"DOI":"10.1371\/journal.pcbi.1005612","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2017,7,7]],"date-time":"2017-07-07T00:00:00Z","timestamp":1499385600000}}],"container-title":["PLOS Computational Biology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pcbi.1005612","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T07:20:58Z","timestamp":1540192858000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pcbi.1005612"}},"subtitle":[],"editor":[{"given":"Martin","family":"Meier-Schellersheim","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2017,6,22]]},"references-count":47,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2017,6,22]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pcbi.1005612","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.pcbi.1005612","asserted-by":"object"}]},"ISSN":["1553-7358"],"issn-type":[{"value":"1553-7358","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,22]]}}}