{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:22:48Z","timestamp":1790400168532,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.pcbi.1006944","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2019,4,23]],"date-time":"2019-04-23T00:00:00Z","timestamp":1555977600000}}],"reference-count":62,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2019,4,11]],"date-time":"2019-04-11T00:00:00Z","timestamp":1554940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.ploscompbiol.org"],"crossmark-restriction":false},"short-container-title":["PLoS Comput Biol"],"DOI":"10.1371\/journal.pcbi.1006944","type":"journal-article","created":{"date-parts":[[2019,4,11]],"date-time":"2019-04-11T15:21:33Z","timestamp":1554996093000},"page":"e1006944","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":13,"title":["Model-based analysis of influenza A virus replication in genetically engineered cell lines elucidates the impact of host cell factors on key kinetic parameters of virus growth"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7922-7595","authenticated-orcid":true,"given":"Tanja","family":"Laske","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mandy","family":"Bachmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Dostert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2187-2057","authenticated-orcid":true,"given":"Alexander","family":"Karlas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2541-6251","authenticated-orcid":true,"given":"Dagmar","family":"Wirth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timo","family":"Frensing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas F.","family":"Meyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hansj\u00f6rg","family":"Hauser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Udo","family":"Reichl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2019,4,11]]},"reference":[{"key":"ref1","unstructured":"WHO. FAQs WHO Estimate of Respiratory Deaths due to Seasonal Influenza. 2018; Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/influenza\/surveillance_monitoring\/bod\/en\/\" xlink:type=\"simple\">http:\/\/www.who.int\/influenza\/surveillance_monitoring\/bod\/en\/<\/ext-link>"},{"key":"ref2","first-page":"1","article-title":"Current Topics in Microbiology and Immunology","author":"ML Shaw","year":"2017"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.tips.2011.10.004","article-title":"Emerging cellular targets for influenza antiviral agents","volume":"33","author":"KH M\u00fcller","year":"2012","journal-title":"Trends Pharmacol Sci"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/j.chom.2014.11.002","article-title":"Influenza Virus-Host Interactome Screen as a Platform for Antiviral Drug Development.","volume":"16","author":"T Watanabe","year":"2014","journal-title":"Cell Host Microbe"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1877","DOI":"10.1007\/s00705-015-2452-9","article-title":"Interplay between influenza A virus and host factors: targets for antiviral intervention","author":"S Tripathi","year":"2015","journal-title":"Archives of Virology"},{"key":"ref6","article-title":"Advancements in Host-Based Interventions for Influenza Treatment.","volume":"9","author":"TF Yip","year":"2018","journal-title":"Front Immunol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1694","DOI":"10.1128\/JVI.01464-15","article-title":"Engineering Enhanced Vaccine Cell Lines To Eradicate Vaccine-Preventable Diseases: the Polio End Game","volume":"90","author":"SMG van der Sanden","year":"2016","journal-title":"J Virol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"2093","DOI":"10.1016\/j.vaccine.2018.03.010","article-title":"Enhancing viral vaccine production using engineered knockout vero cell lines\u2013A second look","volume":"36","author":"F Hoeksema","year":"2018","journal-title":"Vaccine"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1038\/nature08760","article-title":"Genome-wide RNAi screen identifies human host factors crucial for influenza virus replication","volume":"463","author":"A Karlas","year":"2010","journal-title":"Nature"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1038\/nature08699","article-title":"Human host factors required for influenza virus replication","volume":"463","author":"R K\u00f6nig","year":"2010","journal-title":"Nature"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.chom.2010.05.008","article-title":"Cellular networks involved in the influenza virus life cycle","volume":"7","author":"T Watanabe","year":"2010","journal-title":"Cell Host Microbe"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"7806","DOI":"10.1128\/JVI.00080-12","article-title":"Modeling the Intracellular Dynamics of Influenza Virus Replication To Understand the Control of Viral RNA Synthesis","author":"FS Heldt","year":"2012","journal-title":"Journal of Virology"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1038\/nature07151","article-title":"Drosophila RNAi screen identifies host genes important for influenza virus replication","volume":"454","author":"L Hao","year":"2008","journal-title":"Nature"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1016\/j.cell.2009.12.018","article-title":"A Physical and Regulatory Map of Host-Influenza Interactions Reveals Pathways in H1N1 Infection.","volume":"139","author":"SD Shapira","year":"2009","journal-title":"Cell"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1016\/j.cell.2009.12.017","article-title":"The IFITM Proteins Mediate Cellular Resistance to Influenza A H1N1 Virus, West Nile Virus, and Dengue Virus","volume":"139","author":"AL Brass","year":"2009","journal-title":"Cell"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"15109","DOI":"10.1074\/jbc.M111.328070","article-title":"Influenza A virus neuraminidase protein enhances cell survival through interaction with carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) protein","volume":"287","author":"P Gaur","year":"2012","journal-title":"J Biol Chem"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"13010","DOI":"10.1128\/JVI.02651-10","article-title":"Generation and comprehensive analysis of an influenza virus polymerase cellular interaction network","volume":"85","author":"L Tafforeau","year":"2011","journal-title":"J Virol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1743-422X-11-154","article-title":"Influenza polymerase encoding mRNAs utilize atypical mRNA nuclear export","volume":"11","author":"S Larsen","year":"2014","journal-title":"Virol J"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"25405","DOI":"10.1074\/jbc.M114.558817","article-title":"Phospholipase D facilitates efficient entry of influenza virus, allowing escape from innate immune inhibition","volume":"289","author":"TH Oguin","year":"2014","journal-title":"J Biol Chem"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"11330","DOI":"10.1021\/bi00093a009","article-title":"Evaluation of Viral Membrane Fusion Assays. Comparison of the Octadecylrhodamine Dequenching Assay with the Pyrene Excimer Assay","volume":"32","author":"T Stegmann","year":"1993","journal-title":"Biochemistry"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jviromet.2010.12.014","article-title":"Strand-specific real-time RT-PCR for distinguishing influenza vRNA, cRNA, and mRNA","volume":"173","author":"E Kawakami","year":"2011","journal-title":"J Virol Methods"},{"key":"ref22","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1003372","article-title":"Multiscale Modeling of Influenza A Virus Infection Supports the Development of Direct-Acting Antivirals.","volume":"9","author":"FS Heldt","year":"2013","journal-title":"PLoS Comput Biol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.virusres.2008.04.028","article-title":"Maturation efficiency of viral glycoproteins in the ER impacts the production of influenza A virus","volume":"136","author":"M Ueda","year":"2008","journal-title":"Virus Res"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.virol.2017.06.020","article-title":"Influenza A virus cap-snatches host RNAs based on their abundance early after infection","volume":"509","author":"D Sikora","year":"2017","journal-title":"Virology"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"2331","DOI":"10.1016\/j.celrep.2016.05.036","article-title":"CEACAM1-Mediated Inhibition of Virus Production","volume":"15","author":"A Vitenshtein","year":"2016","journal-title":"Cell Rep"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"2973","DOI":"10.1007\/s00018-015-1879-1","article-title":"Influenza infection induces host DNA damage and dynamic DNA damage responses during tissue regeneration","volume":"72","author":"N Li","year":"2015","journal-title":"Cell Mol life Sci"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1074\/jbc.M706647200","article-title":"Isolation of XAB2 complex involved in pre-mRNA splicing, transcription, and transcription-coupled repair","volume":"283","author":"I Kuraoka","year":"2008","journal-title":"J Biol Chem"},{"key":"ref28","first-page":"1377","article-title":"VhfRNAi: A web-platform for analysis of host genes involved in viral infections discovered by genome wide RNAi screens. Mol Biosyst","volume":"13","author":"A Thakur","year":"2017","journal-title":"Royal Society of Chemistry"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"8938","DOI":"10.1038\/ncomms9938","article-title":"Single-cell analysis and stochastic modelling unveil large cell-to-cell variability in influenza A virus infection","volume":"6","author":"FS Heldt","year":"2015","journal-title":"Nat Commun"},{"key":"ref30","first-page":"01786","article-title":"A novel type of influenza A virus-derived defective interfering particle with nucleotide substitutions in its genome","author":"SY Kupke","year":"2018","journal-title":"J Virol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"10651","DOI":"10.1128\/JVI.07244-11","article-title":"The H275Y Neuraminidase Mutation of the Pandemic A\/H1N1 Influenza Virus Lengthens the Eclipse Phase and Reduces Viral Output of Infected Cells, Potentially Compromising Fitness in Ferrets","volume":"86","author":"LT Pinilla","year":"2012","journal-title":"J Virol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/srep24154","article-title":"Avian influenza viruses that cause highly virulent infections in humans exhibit distinct replicative properties in contrast to human H1N1 viruses","volume":"6","author":"PF Simon","year":"2016","journal-title":"Sci Rep."},{"key":"ref33","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.virusres.2015.09.011","article-title":"Multi-scale model for hepatitis C viral load kinetics under treatment with direct acting antivirals","volume":"218","author":"D Clausznitzer","year":"2015","journal-title":"Virus Res"},{"key":"ref34","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1003561","article-title":"Replication Vesicles are Load- and Choke-Points in the Hepatitis C Virus Lifecycle","volume":"9","author":"M Binder","year":"2013","journal-title":"PLoS Pathog"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.virusres.2015.11.016","article-title":"Modeling the intracellular replication of influenza A virus in the presence of defective interfering RNAs","volume":"213","author":"T Laske","year":"2016","journal-title":"Virus Res"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"303","DOI":"10.2217\/fmb.09.121","article-title":"The use of RNAi-based screens to identify host proteins involved in viral replication","volume":"5","author":"AJ Hirsch","year":"2010","journal-title":"Future Microbiol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.micinf.2011.01.012","article-title":"Uncovering the global host cell requirements for influenza virus replication via RNAi screening","volume":"13","author":"S Stertz","year":"2011","journal-title":"Microbes Infect"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.mib.2015.06.001","article-title":"Recent strategies and progress in identifying host factors involved in virus replication","volume":"26","author":"R K\u00f6nig","year":"2015","journal-title":"Curr Opin Microbiol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"3259","DOI":"10.1038\/ncomms4259","article-title":"Fragile X mental retardation protein stimulates ribonucleoprotein assembly of influenza A virus","volume":"5","author":"Z Zhou","year":"2014","journal-title":"Nat Commun"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"e1005013","DOI":"10.1371\/journal.pcbi.1005013","article-title":"Integrating Transcriptomic and Proteomic Data Using Predictive Regulatory Network Models of Host Response to Pathogens","volume":"12","author":"D Chasman","year":"2016","journal-title":"PLOS Comput Biol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/j.chom.2015.11.002","article-title":"Meta- and Orthogonal Integration of Influenza \u201cOMICs\u201d Data Defines a Role for UBR4 in Virus Budding","volume":"18","author":"S Tripathi","year":"2015","journal-title":"Cell Host Microbe"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"E4324","DOI":"10.1073\/pnas.1701670114","article-title":"Energetic cost of building a virus","volume":"114","author":"G Mahmoudabadi","year":"2017","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.copbio.2007.07.013","article-title":"Road to precision: recombinase-based targeting technologies for genome engineering","volume":"18","author":"D Wirth","year":"2007","journal-title":"Curr Opin Biotechnol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1146\/annurev-biochem-060815-014607","article-title":"CRISPR\/Cas9 in Genome Editing and Beyond","volume":"85","author":"H Wang","year":"2016","journal-title":"Annu Rev Biochem"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"5547","DOI":"10.1073\/pnas.89.12.5547","article-title":"Tight control of gene expression in mammalian cells by tetracycline-responsive promoters","volume":"89","author":"M Gossen","year":"1992","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref46","first-page":"451","article-title":"Methods in molecular biology","author":"W Weber","year":"2004"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1214\/ss\/1177013815","article-title":"Bootstrap Methods for Standard Errors, Confidence Intervals, and Other Measures of Statistical Accuracy","volume":"1","author":"B Efron","year":"1986","journal-title":"Stat Sci"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1038\/nrmicro1263","article-title":"Genome-wide analysis of retroviral DNA integration","volume":"3","author":"F Bushman","year":"2005","journal-title":"Nat Rev Microbiol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1063\/1.4822377","article-title":"CVODE, a stiff\/nonstiff ODE solver in C.","volume":"10","author":"SD Cohen","year":"1996","journal-title":"Comput Phys"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1093\/bioinformatics\/bti799","article-title":"Systems Biology Toolbox for MATLAB: A computational platform for research in systems biology","volume":"22","author":"H Schmidt","year":"2006","journal-title":"Bioinformatics"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1007\/s10898-006-9075-3","article-title":"Scatter search for chemical and bio-process optimization.","volume":"37","author":"JA Egea","year":"2007","journal-title":"J Glob Optim"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"8957","DOI":"10.1128\/JVI.77.16.8957-8951.2003","article-title":"Conditional Suppression of Cellular Genes: Lentivirus Vector-Mediated Drug-Inducible RNA Interference","volume":"77","author":"M Wiznerowicz","year":"2003","journal-title":"J Virol"},{"key":"ref53","first-page":"1","article-title":"Bimodal and hysteretic expression in mamalian cells from a synthetic gene circuit","volume":"3","author":"T May","year":"2008","journal-title":"PLoS One"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1093\/jnci\/51.5.1417","article-title":"In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors","volume":"51","author":"DJ Giard","year":"1973","journal-title":"J Natl Cancer Inst"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1002\/ijc.2910170110","article-title":"A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells","volume":"17","author":"M Lieber","year":"1976","journal-title":"Int J Cancer"},{"key":"ref56","first-page":"457","article-title":"Animal Cell Biotechnology: Methods and Protocols","author":"Y Genzel","year":"2007"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.biologicals.2007.10.002","article-title":"Monitoring influenza virus content in vaccine production: Precise assays for the quantitation of hemagglutination and neuraminidase activity","volume":"36","author":"B Kalbfuss","year":"2008","journal-title":"Biologicals"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1084\/jem.100.2.203","article-title":"Morphological and quantitative comparison between infectious and non-infectious forms of influenza virus","volume":"100","author":"GH Werner","year":"1954","journal-title":"J Exp Med"},{"key":"ref59","author":"BWJ Mahy","year":"1996"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"7181","DOI":"10.1007\/s00253-016-7542-4","article-title":"Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells","volume":"100","author":"T Frensing","year":"2016","journal-title":"Appl Microbiol Biotechnol"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"1422","DOI":"10.1016\/j.micinf.2007.07.007","article-title":"Visualization of microtubule-mediated transport of influenza viral progeny ribonucleoprotein","volume":"9","author":"F Momose","year":"2007","journal-title":"Microbes Infect"}],"updated-by":[{"DOI":"10.1371\/journal.pcbi.1006944","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2019,4,23]],"date-time":"2019-04-23T00:00:00Z","timestamp":1555977600000}}],"container-title":["PLOS Computational Biology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.pcbi.1006944","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T18:51:54Z","timestamp":1588963914000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pcbi.1006944"}},"subtitle":[],"editor":[{"given":"James","family":"Lloyd-Smith","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,4,11]]},"references-count":62,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2019,4,11]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pcbi.1006944","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.pcbi.1006944","asserted-by":"object"}]},"ISSN":["1553-7358"],"issn-type":[{"value":"1553-7358","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,11]]}}}