{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T09:29:00Z","timestamp":1768469340280,"version":"3.49.0"},"update-to":[{"DOI":"10.1371\/journal.pcbi.1008482","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2020,12,16]],"date-time":"2020-12-16T00:00:00Z","timestamp":1608076800000}}],"reference-count":34,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2020,12,4]],"date-time":"2020-12-04T00:00:00Z","timestamp":1607040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"crossref","award":["06294-2019-RGPIN"],"award-info":[{"award-number":["06294-2019-RGPIN"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.ploscompbiol.org"],"crossmark-restriction":false},"short-container-title":["PLoS Comput Biol"],"abstract":"<jats:p>Integrated into their bacterial hosts\u2019 genomes, prophage sequences exhibit a wide diversity of length and gene content, from highly degraded cryptic sequences to intact, functional prophages that retain a full complement of lytic-function genes. We apply three approaches\u2014bioinformatics, analytical modelling and computational simulation\u2014to understand the diverse gene content of prophages. In the bioinformatics work, we examine the distributions of over 50,000 annotated prophage genes identified in 1384 prophage sequences, comparing the gene repertoires of intact and incomplete prophages. These data indicate that genes involved in the replication, packaging, and release of phage particles have been preferentially lost in incomplete prophages, while tail fiber, transposase and integrase genes are significantly enriched. Consistent with these results, our mathematical and computational approaches predict that genes involved in phage lytic function are preferentially lost, resulting in shorter prophages that often retain genes that benefit the host. Informed by these models, we offer novel hypotheses for the enrichment of integrase and transposase genes in cryptic prophages. Overall, we demonstrate that functional and cryptic prophages represent a diversity of genetic sequences that evolve along a parasitism-mutualism continuum.<\/jats:p>","DOI":"10.1371\/journal.pcbi.1008482","type":"journal-article","created":{"date-parts":[[2020,12,4]],"date-time":"2020-12-04T20:29:27Z","timestamp":1607113767000},"page":"e1008482","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["Evolution along the parasitism-mutualism continuum determines the genetic repertoire of prophages"],"prefix":"10.1371","volume":"16","author":[{"given":"Amjad","family":"Khan","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4304-5551","authenticated-orcid":true,"given":"Alita R.","family":"Burmeister","sequence":"additional","affiliation":[]},{"given":"Lindi 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