{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T07:25:13Z","timestamp":1778743513781,"version":"3.51.4"},"update-to":[{"DOI":"10.1371\/journal.pcbi.1008704","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T00:00:00Z","timestamp":1616025600000}}],"reference-count":65,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T00:00:00Z","timestamp":1615161600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.ploscompbiol.org"],"crossmark-restriction":false},"short-container-title":["PLoS Comput Biol"],"abstract":"<jats:p>\n                    Acrylic acid is a value-added chemical used in industry to produce diapers, coatings, paints, and adhesives, among many others. Due to its economic importance, there is currently a need for new and sustainable ways to synthesise it. Recently, the focus has been laid in the use of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    to express the full bio-based pathway using 3-hydroxypropionate as an intermediary through three distinct pathways (glycerol, malonyl-CoA, and\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -alanine). Hence, the goals of this work were to use COPASI software to assess which of the three pathways has a higher potential for industrial-scale production, from either glucose or glycerol, and identify potential targets to improve the biosynthetic pathways yields. When compared to the available literature, the models developed during this work successfully predict the production of 3-hydroxypropionate, using glycerol as carbon source in the glycerol pathway, and using glucose as a carbon source in the malonyl-CoA and\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -alanine pathways. Finally, this work allowed to identify four potential over-expression targets (glycerol-3-phosphate dehydrogenase (G3pD), acetyl-CoA carboxylase (AccC), aspartate aminotransferase (AspAT), and aspartate carboxylase (AspC)) that should, theoretically, result in higher AA yields.\n                  <\/jats:p>","DOI":"10.1371\/journal.pcbi.1008704","type":"journal-article","created":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T13:36:34Z","timestamp":1615210594000},"page":"e1008704","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["A kinetic model of the central carbon metabolism for acrylic acid production in Escherichia coli"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2255-8983","authenticated-orcid":true,"given":"Alexandre","family":"Oliveira","sequence":"first","affiliation":[]},{"given":"Joana","family":"Rodrigues","sequence":"additional","affiliation":[]},{"given":"Eug\u00e9nio Campos","family":"Ferreira","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9265-0630","authenticated-orcid":true,"given":"L\u00edgia","family":"Rodrigues","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1765-7178","authenticated-orcid":true,"given":"Oscar","family":"Dias","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2021,3,8]]},"reference":[{"key":"pcbi.1008704.ref001","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.ymben.2015.08.005","article-title":"Direct fermentation route for the production of acrylic acid","volume":"32","author":"HS Chu","year":"2015","journal-title":"Metab Eng"},{"key":"pcbi.1008704.ref002","doi-asserted-by":"crossref","first-page":"4901","DOI":"10.1007\/s00253-015-7272-z","article-title":"Biosynthetic pathway for acrylic acid from glycerol in recombinant Escherichia coli","volume":"100","author":"W Tong","year":"2016","journal-title":"Appl Microbiol Biotechnol"},{"key":"pcbi.1008704.ref003","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1039\/C4GC02076F","article-title":"Catalytic routes towards acrylic acid, adipic acid and \u03b5-caprolactam starting from biorenewables","volume":"17","author":"R Beerthuis","year":"2015","journal-title":"Green Chem"},{"key":"pcbi.1008704.ref004","unstructured":"Allied Market Research. Acrylic Acid Market Report. 2016 [cited 20 Oct 2019]. 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