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We performed a direct three-dimensional simulation on the behaviors of the tumor cells in a real microvascular network, by a hybrid method of the smoothed dissipative particle dynamics and immersed boundary method (SDPD-IBM). The tumor cells are found to adhere at the microvascular bifurcations more frequently, and there is a positive correlation between the adhesion of the tumor cells and the wall-directed force from the surrounding red blood cells (RBCs). The larger the wall-directed force is, the closer the tumor cells are marginated towards the wall, and the higher the probability of adhesion behavior happen is. A relatively low or high hematocrit can help to prevent the adhesion of tumor cells, and similarly, increasing the shear rate of blood flow can serve the same purpose. These results suggest that the tumor cells may be more likely to extravasate at the microvascular bifurcations if the blood flow is slow and the hematocrit is moderate.<\/jats:p>","DOI":"10.1371\/journal.pcbi.1008746","type":"journal-article","created":{"date-parts":[[2021,2,20]],"date-time":"2021-02-20T05:16:01Z","timestamp":1613798161000},"page":"e1008746","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":47,"title":["Margination and adhesion dynamics of tumor cells in a real microvascular 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Law","volume":"78","author":"TF Sherman","year":"1981","journal-title":"J Gen Physiol"},{"key":"pcbi.1008746.ref050","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/0026-2862(72)90069-6","article-title":"Improved measurements of the erythrocyte geometry","volume":"4","author":"E Evans","year":"1972","journal-title":"Microvasc Res"},{"key":"pcbi.1008746.ref051","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1007\/s00384-009-0694-2","article-title":"Metastatic tumor cell arrest in the liver-lumen occlusion and specific adhesion are not exclusive","volume":"24","author":"P Gassmann","year":"2009","journal-title":"Int J Colorectal Dis"},{"key":"pcbi.1008746.ref052","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1007\/s10237-011-0339-6","article-title":"Effects of wall shear stress and its gradient on tumor cell adhesion in curved microvessels","volume":"11","author":"WW Yan","year":"2012","journal-title":"Biomech Model 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