{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T21:21:49Z","timestamp":1768771309837,"version":"3.49.0"},"update-to":[{"DOI":"10.1371\/journal.pcbi.1008855","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,4,8]],"date-time":"2021-04-08T00:00:00Z","timestamp":1617840000000}}],"reference-count":70,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2021,3,29]],"date-time":"2021-03-29T00:00:00Z","timestamp":1616976000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["He 3875\/15-1"],"award-info":[{"award-number":["He 3875\/15-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.ploscompbiol.org"],"crossmark-restriction":false},"short-container-title":["PLoS Comput Biol"],"abstract":"<jats:p>The Sec complex catalyzes the translocation of proteins of the secretory pathway into the endoplasmic reticulum and the integration of membrane proteins into the endoplasmic reticulum membrane. Some substrate peptides require the presence and involvement of accessory proteins such as Sec63. Recently, a structure of the Sec complex from <jats:italic>Saccharomyces cerevisiae<\/jats:italic>, consisting of the Sec61 channel and the Sec62, Sec63, Sec71 and Sec72 proteins was determined by cryo-electron microscopy (cryo-EM). Here, we show by co-precipitation that the Sec61 channel subunit Sbh1 is not required for formation of stable Sec63-Sec61 contacts. Molecular dynamics simulations started from the cryo-EM conformation of Sec61 bound to Sec63 and of unbound Sec61 revealed how Sec63 affects the conformation of Sec61 lateral gate, plug, pore region and pore ring diameter via three intermolecular contact regions. Molecular docking of SRP-dependent vs. SRP-independent signal peptide chains into the Sec61 channel showed that the pore regions affected by presence\/absence of Sec63 play a crucial role in positioning the signal anchors of SRP-dependent substrates nearby the lateral gate.<\/jats:p>","DOI":"10.1371\/journal.pcbi.1008855","type":"journal-article","created":{"date-parts":[[2021,3,29]],"date-time":"2021-03-29T17:39:11Z","timestamp":1617039551000},"page":"e1008855","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["How does Sec63 affect the conformation of Sec61 in yeast?"],"prefix":"10.1371","volume":"17","author":[{"given":"Pratiti","family":"Bhadra","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6614-7000","authenticated-orcid":true,"given":"Lalitha","family":"Yadhanapudi","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0415-6558","authenticated-orcid":true,"given":"Karin","family":"R\u00f6misch","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2180-9154","authenticated-orcid":true,"given":"Volkhard","family":"Helms","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2021,3,29]]},"reference":[{"key":"pcbi.1008855.ref001","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1146\/annurev-biophys-050511-102312","article-title":"Mechanisms of Sec61\/SecY-mediated protein translocation across membranes","volume":"41","author":"E Park","year":"2012","journal-title":"Annual review of biophysics"},{"key":"pcbi.1008855.ref002","doi-asserted-by":"crossref","first-page":"887","DOI":"10.3389\/fphys.2017.00887","article-title":"An update on Sec61 channel functions, mechanisms, and related diseases","volume":"8","author":"S Lang","year":"2017","journal-title":"Frontiers in Physiology"},{"issue":"3","key":"pcbi.1008855.ref003","doi-asserted-by":"crossref","DOI":"10.1242\/jcs.231340","article-title":"A clearer picture of the ER translocon complex","volume":"133","author":"M Gemmer","year":"2020","journal-title":"Journal of Cell Science"},{"issue":"1","key":"pcbi.1008855.ref004","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/sigtrans.2017.2","article-title":"Let\u2019s talk about Secs: Sec61, Sec62 and Sec63 in signal transduction, oncology and personalized medicine","volume":"2","author":"M Linxweiler","year":"2017","journal-title":"Signal transduction and targeted therapy"},{"issue":"6422","key":"pcbi.1008855.ref005","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1126\/science.aav6740","article-title":"Structure of the posttranslational Sec protein-translocation channel complex from yeast","volume":"363","author":"S Itskanov","year":"2019","journal-title":"Science"},{"issue":"7742","key":"pcbi.1008855.ref006","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1038\/s41586-018-0856-x","article-title":"Structure of the post-translational protein translocation machinery of the ER membrane","volume":"566","author":"X Wu","year":"2019","journal-title":"Nature"},{"issue":"6","key":"pcbi.1008855.ref007","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.tibs.2019.03.003","article-title":"Post-translational protein transport by the Sec complex","volume":"44","author":"WJ Allen","year":"2019","journal-title":"Trends in biochemical sciences"},{"issue":"4","key":"pcbi.1008855.ref008","doi-asserted-by":"crossref","first-page":"2956","DOI":"10.1074\/jbc.M110.159517","article-title":"Structural studies and the assembly of the heptameric post-translational translocon complex","volume":"286","author":"Y Harada","year":"2011","journal-title":"Journal of Biological Chemistry"},{"issue":"13","key":"pcbi.1008855.ref009","doi-asserted-by":"crossref","first-page":"7214","DOI":"10.1073\/pnas.97.13.7214","article-title":"Homologs of the yeast Sec complex subunits Sec62p and Sec63p are abundant proteins in dog pancreas microsomes","volume":"97","author":"J Tyedmers","year":"2000","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"10","key":"pcbi.1008855.ref010","doi-asserted-by":"crossref","first-page":"1662","DOI":"10.1091\/mbc.e10-01-0060","article-title":"The hydrophobic core of the Sec61 translocon defines the hydrophobicity threshold for membrane integration","volume":"21","author":"T Junne","year":"2010","journal-title":"Molecular biology of the cell"},{"issue":"23","key":"pcbi.1008855.ref011","doi-asserted-by":"crossref","first-page":"7171","DOI":"10.1021\/bi034395l","article-title":"An in vitro assay using overexpressed yeast SRP demonstrates that cotranslational translocation is dependent upon the J-domain of Sec63p","volume":"42","author":"M Willer","year":"2003","journal-title":"Biochemistry"},{"issue":"1","key":"pcbi.1008855.ref012","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/ncomms10133","article-title":"Mammalian SRP receptor switches the Sec61 translocase from Sec62 to SRP-dependent translocation","volume":"6","author":"B Jadhav","year":"2015","journal-title":"Nature communications"},{"issue":"5","key":"pcbi.1008855.ref013","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/S0092-8674(00)80767-9","article-title":"BiP acts as a molecular ratchet during posttranslational transport of prepro-\u03b1 factor across the ER membrane","volume":"97","author":"KE Matlack","year":"1999","journal-title":"Cell"},{"issue":"11","key":"pcbi.1008855.ref014","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.1016\/j.bbamcr.2018.08.018","article-title":"Profiling of signal sequence characteristics and requirement of different translocation components","volume":"1865","author":"C Yim","year":"2018","journal-title":"Biochimica et Biophysica Acta (BBA)-Molecular Cell Research"},{"issue":"9","key":"pcbi.1008855.ref015","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1016\/j.bbagen.2019.06.005","article-title":"Proper insertion and topogenesis of membrane proteins in the ER depend on Sec63","volume":"1863","author":"SJ Jung","year":"2019","journal-title":"Biochimica et Biophysica Acta (BBA)-General Subjects"},{"issue":"19","key":"pcbi.1008855.ref016","doi-asserted-by":"crossref","first-page":"4270","DOI":"10.1242\/jcs.153650","article-title":"The Sec62\u2013Sec63 translocon facilitates translocation of the C-terminus of membrane proteins","volume":"127","author":"SJ Jung","year":"2014","journal-title":"Journal of cell science"},{"issue":"2","key":"pcbi.1008855.ref017","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1083\/jcb.134.2.269","article-title":"Signal sequences specify the targeting route to the endoplasmic reticulum membrane","volume":"134","author":"D Ng","year":"1996","journal-title":"The Journal of cell biology"},{"issue":"7","key":"pcbi.1008855.ref018","doi-asserted-by":"crossref","first-page":"1632","DOI":"10.1016\/j.cell.2014.05.024","article-title":"Structure of the mammalian ribosome-Sec61 complex to 3.4 \u00c5 resolution","volume":"157","author":"RM Voorhees","year":"2014","journal-title":"Cell"},{"issue":"6268","key":"pcbi.1008855.ref019","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1126\/science.aad4992","article-title":"Structure of the Sec61 channel opened by a signal sequence","volume":"351","author":"RM Voorhees","year":"2016","journal-title":"Science"},{"issue":"8","key":"pcbi.1008855.ref020","doi-asserted-by":"crossref","first-page":"2718","DOI":"10.1529\/biophysj.105.073304","article-title":"Size, motion, and function of the SecY translocon revealed by molecular dynamics simulations with virtual probes","volume":"90","author":"P Tian","year":"2006","journal-title":"Biophysical journal"},{"issue":"7","key":"pcbi.1008855.ref021","doi-asserted-by":"crossref","first-page":"2356","DOI":"10.1529\/biophysj.105.075291","article-title":"Molecular dynamics studies of the archaeal translocon","volume":"90","author":"J Gumbart","year":"2006","journal-title":"Biophysical journal"},{"issue":"39","key":"pcbi.1008855.ref022","doi-asserted-by":"crossref","first-page":"11147","DOI":"10.1021\/bi700835d","article-title":"Structural determinants of lateral gate opening in the protein translocon","volume":"46","author":"J Gumbart","year":"2007","journal-title":"Biochemistry"},{"issue":"11","key":"pcbi.1008855.ref023","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1016\/j.str.2009.09.010","article-title":"Regulation of the protein-conducting channel by a bound ribosome","volume":"17","author":"J Gumbart","year":"2009","journal-title":"Structure"},{"issue":"28","key":"pcbi.1008855.ref024","doi-asserted-by":"crossref","first-page":"11588","DOI":"10.1073\/pnas.0900638106","article-title":"Insertion of short transmembrane helices by the Sec61 translocon","volume":"106","author":"S Jaud","year":"2009","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"47","key":"pcbi.1008855.ref025","doi-asserted-by":"crossref","first-page":"18856","DOI":"10.1073\/pnas.1318432110","article-title":"Functional asymmetry within the Sec61p translocon","volume":"110","author":"E Demirci","year":"2013","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"26","key":"pcbi.1008855.ref026","doi-asserted-by":"crossref","first-page":"14876","DOI":"10.1039\/C9RA01684H","article-title":"A molecular dynamics study on the resilience of Sec61 channel from open to closed state","volume":"9","author":"S Sun","year":"2019","journal-title":"RSC advances"},{"issue":"12","key":"pcbi.1008855.ref027","doi-asserted-by":"crossref","first-page":"5399","DOI":"10.1073\/pnas.0914752107","article-title":"Hydrophobically stabilized open state for the lateral gate of the Sec translocon","volume":"107","author":"B Zhang","year":"2010","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"2","key":"pcbi.1008855.ref028","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1073\/pnas.1220361110","article-title":"Exploring the nature of the translocon-assisted protein insertion","volume":"110","author":"A Rychkova","year":"2013","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"4","key":"pcbi.1008855.ref029","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1016\/j.celrep.2012.08.039","article-title":"Long-timescale dynamics and regulation of Sec-facilitated protein translocation","volume":"2","author":"B Zhang","year":"2012","journal-title":"Cell reports"},{"issue":"3","key":"pcbi.1008855.ref030","doi-asserted-by":"crossref","first-page":"e1005427","DOI":"10.1371\/journal.pcbi.1005427","article-title":"Structurally detailed coarse-grained model for Sec-facilitated co-translational protein translocation and membrane integration","volume":"13","author":"MJ Niesen","year":"2017","journal-title":"PLoS computational biology"},{"issue":"6","key":"pcbi.1008855.ref031","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/S0092-8674(00)81738-9","article-title":"Signal sequence recognition in posttranslational protein transport across the yeast ER membrane","volume":"94","author":"K Plath","year":"1998","journal-title":"Cell"},{"issue":"1","key":"pcbi.1008855.ref032","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1091\/mbc.e03-06-0390","article-title":"Interactions between Sec complex and prepro-\u03b1-factor during posttranslational protein transport into the endoplasmic reticulum","volume":"15","author":"K Plath","year":"2004","journal-title":"Molecular biology of the cell"},{"issue":"5958","key":"pcbi.1008855.ref033","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1126\/science.1178535","article-title":"Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome","volume":"326","author":"T Becker","year":"2009","journal-title":"Science"},{"issue":"1","key":"pcbi.1008855.ref034","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1002\/cpbi.3","article-title":"Comparative protein structure modeling using MODELLER","volume":"54","author":"B Webb","year":"2016","journal-title":"Current protocols in bioinformatics"},{"issue":"1","key":"pcbi.1008855.ref035","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/1471-2121-13-34","article-title":"N-acetylation and phosphorylation of Sec complex subunits in the ER membrane","volume":"13","author":"C Soromani","year":"2012","journal-title":"BMC cell biology"},{"issue":"4","key":"pcbi.1008855.ref036","doi-asserted-by":"crossref","first-page":"e0215950","DOI":"10.1371\/journal.pone.0215950","article-title":"The N-terminus of Sec61p plays key roles in ER protein import and ERAD","volume":"14","author":"F Elia","year":"2019","journal-title":"Plos one"},{"issue":"suppl_2","key":"pcbi.1008855.ref037","doi-asserted-by":"crossref","first-page":"W665","DOI":"10.1093\/nar\/gkh381","article-title":"PDB2PQR: an automated pipeline for the setup of Poisson\u2013Boltzmann electrostatics calculations","volume":"32","author":"TJ Dolinsky","year":"2004","journal-title":"Nucleic acids research"},{"issue":"9","key":"pcbi.1008855.ref038","doi-asserted-by":"crossref","first-page":"e880","DOI":"10.1371\/journal.pone.0000880","article-title":"Automated builder and database of protein\/membrane complexes for molecular dynamics simulations","volume":"2","author":"S Jo","year":"2007","journal-title":"PloS one"},{"issue":"27","key":"pcbi.1008855.ref039","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1002\/jcc.23702","article-title":"CHARMM-GUI membrane builder toward realistic biological membrane simulations","volume":"35","author":"EL Wu","year":"2014","journal-title":"Journal of computational chemistry"},{"issue":"D1","key":"pcbi.1008855.ref040","doi-asserted-by":"crossref","first-page":"D370","DOI":"10.1093\/nar\/gkr703","article-title":"OPM database and PPM web server: resources for positioning of proteins in membranes","volume":"40","author":"MA Lomize","year":"2012","journal-title":"Nucleic acids research"},{"issue":"16","key":"pcbi.1008855.ref041","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1002\/jcc.20291","article-title":"GROMACS: fast, flexible, and free","volume":"26","author":"D Van Der Spoel","year":"2005","journal-title":"Journal of computational chemistry"},{"issue":"23","key":"pcbi.1008855.ref042","doi-asserted-by":"crossref","first-page":"7830","DOI":"10.1021\/jp101759q","article-title":"Update of the CHARMM all-atom additive force field for lipids: validation on six lipid types","volume":"114","author":"JB Klauda","year":"2010","journal-title":"The journal of physical chemistry B"},{"issue":"9","key":"pcbi.1008855.ref043","doi-asserted-by":"crossref","first-page":"3257","DOI":"10.1021\/ct300400x","article-title":"Optimization of the additive CHARMM all-atom protein force field targeting improved sampling of the backbone \u03d5, \u03c8 and side-chain \u03c71 and \u03c72 dihedral angles","volume":"8","author":"RB Best","year":"2012","journal-title":"Journal of chemical theory and computation"},{"issue":"2","key":"pcbi.1008855.ref044","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1063\/1.445869","article-title":"Comparison of simple potential functions for simulating liquid water","volume":"79","author":"WL Jorgensen","year":"1983","journal-title":"The Journal of chemical physics"},{"issue":"1","key":"pcbi.1008855.ref045","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0263-7855(96)00018-5","article-title":"VMD: visual molecular dynamics","volume":"14","author":"W Humphrey","year":"1996","journal-title":"Journal of molecular graphics"},{"issue":"12","key":"pcbi.1008855.ref046","doi-asserted-by":"crossref","first-page":"10089","DOI":"10.1063\/1.464397","article-title":"Particle mesh Ewald: An N\u22c5 log (N) method for Ewald sums in large systems","volume":"98","author":"T Darden","year":"1993","journal-title":"The Journal of chemical physics"},{"issue":"8","key":"pcbi.1008855.ref047","doi-asserted-by":"crossref","first-page":"4069","DOI":"10.1063\/1.449071","article-title":"The nose\u2013hoover thermostat","volume":"83","author":"DJ Evans","year":"1985","journal-title":"The Journal of chemical physics"},{"issue":"12","key":"pcbi.1008855.ref048","doi-asserted-by":"crossref","first-page":"7182","DOI":"10.1063\/1.328693","article-title":"Polymorphic transitions in single crystals: A new molecular dynamics method","volume":"52","author":"M Parrinello","year":"1981","journal-title":"Journal of Applied physics"},{"issue":"12","key":"pcbi.1008855.ref049","doi-asserted-by":"crossref","first-page":"2577","DOI":"10.1002\/bip.360221211","article-title":"Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features","volume":"22","author":"W Kabsch","year":"1983","journal-title":"Biopolymers: Original Research on Biomolecules"},{"issue":"1-2","key":"pcbi.1008855.ref050","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1093\/emboj\/20.1.262","article-title":"Sec63p and Kar2p are required for the translocation of SRP-dependent precursors into the yeast endoplasmic reticulum in vivo","volume":"20","author":"BP Young","year":"2001","journal-title":"The EMBO journal"},{"issue":"5","key":"pcbi.1008855.ref051","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1016\/j.cell.2013.02.003","article-title":"A network of cytosolic factors targets SRP-independent proteins to the endoplasmic reticulum","volume":"152","author":"T Ast","year":"2013","journal-title":"Cell"},{"issue":"24","key":"pcbi.1008855.ref052","doi-asserted-by":"crossref","first-page":"5121","DOI":"10.1093\/bioinformatics\/btz459","article-title":"AutoDock CrankPep: combining folding and docking to predict protein\u2013peptide complexes","volume":"35","author":"Y Zhang","year":"2019","journal-title":"Bioinformatics"},{"issue":"1","key":"pcbi.1008855.ref053","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1751-0473-3-12","article-title":"CRANKITE: a fast polypeptide backbone conformation sampler","volume":"3","author":"AA Podtelezhnikov","year":"2008","journal-title":"Source Code for Biology and Medicine"},{"issue":"42","key":"pcbi.1008855.ref054","doi-asserted-by":"crossref","first-page":"30618","DOI":"10.1074\/jbc.M701840200","article-title":"The transmembrane domain is sufficient for Sbh1p function, its association with the Sec61 complex, and interaction with Rtn1p","volume":"282","author":"D Feng","year":"2007","journal-title":"Journal of Biological Chemistry"},{"issue":"12","key":"pcbi.1008855.ref055","doi-asserted-by":"crossref","first-page":"3455","DOI":"10.1091\/mbc.9.12.3455","article-title":"Sec61p serves multiple roles in secretory precursor binding and translocation into the endoplasmic reticulum membrane","volume":"9","author":"M Pilon","year":"1998","journal-title":"Molecular biology of the cell"},{"issue":"2","key":"pcbi.1008855.ref056","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1083\/jcb.101.2.460","article-title":"Processing of the asparagine-linked oligosaccharides of secreted and intracellular forms of the vesicular stomatitis virus G protein: in vivo evidence of Golgi apparatus compartmentalization","volume":"101","author":"CA Gabel","year":"1985","journal-title":"The Journal of cell biology"},{"issue":"9","key":"pcbi.1008855.ref057","doi-asserted-by":"crossref","first-page":"4063","DOI":"10.1091\/mbc.e06-03-0200","article-title":"The plug domain of yeast Sec61p is important for efficient protein translocation, but is not essential for cell viability","volume":"17","author":"T Junne","year":"2006","journal-title":"Molecular biology of the cell"},{"issue":"26","key":"pcbi.1008855.ref058","doi-asserted-by":"crossref","first-page":"8799","DOI":"10.1021\/jp1029163","article-title":"Single mutation effects on conformational change and membrane deformation of influenza hemagglutinin fusion peptides","volume":"114","author":"J Li","year":"2010","journal-title":"The Journal of Physical Chemistry B"},{"issue":"19","key":"pcbi.1008855.ref059","doi-asserted-by":"crossref","first-page":"3380","DOI":"10.1038\/sj.emboj.7600804","article-title":"Investigating the SecY plug movement at the SecYEG translocation channel","volume":"24","author":"PC Tam","year":"2005","journal-title":"The EMBO journal"},{"issue":"31","key":"pcbi.1008855.ref060","doi-asserted-by":"crossref","first-page":"23747","DOI":"10.1074\/jbc.M110.124636","article-title":"Immobilization of the plug domain inside the SecY channel allows unrestricted protein translocation","volume":"285","author":"JAL a Nijeholt","year":"2010","journal-title":"Journal of Biological Chemistry"},{"issue":"4","key":"pcbi.1008855.ref061","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1016\/j.str.2016.02.001","article-title":"Unlocking the bacterial SecY translocon","volume":"24","author":"RA Corey","year":"2016","journal-title":"Structure"},{"issue":"6","key":"pcbi.1008855.ref062","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1083\/jcb.201207163","article-title":"A gating motif in the translocation channel sets the hydrophobicity threshold for signal sequence function","volume":"199","author":"SF Trueman","year":"2012","journal-title":"Journal of Cell Biology"},{"key":"pcbi.1008855.ref063","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1038\/s41594-020-00541-x","article-title":"Stepwise gating of the Sec61 protein-conducting channel by Sec63 and Sec62","volume":"28","author":"S Itskanov","year":"2021","journal-title":"Nature Structural & Molecular Biology"},{"key":"pcbi.1008855.ref064","first-page":"e105642","article-title":"Architecture of the active post-translational Sec translocon","volume":"40","author":"TH Weng","year":"2021","journal-title":"The EMBO Journal"},{"issue":"3","key":"pcbi.1008855.ref065","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1016\/j.bbrc.2010.04.168","article-title":"The mammalian and yeast translocon complexes comprise a characteristic Sec61 channel","volume":"396","author":"F Erdmann","year":"2010","journal-title":"Biochemical and biophysical research communications"},{"issue":"3","key":"pcbi.1008855.ref066","doi-asserted-by":"crossref","first-page":"228","DOI":"10.4161\/chan.5.3.15314","article-title":"Sec61 complexes form ubiquitous ER Ca2+ leak channels","volume":"5","author":"S Lang","year":"2011","journal-title":"Channels"},{"issue":"7","key":"pcbi.1008855.ref067","doi-asserted-by":"crossref","first-page":"2125","DOI":"10.1074\/jbc.RA119.010378","article-title":"The conserved C-terminus of Sss1p is required to maintain the endoplasmic reticulum permeability barrier","volume":"295","author":"CM Witham","year":"2020","journal-title":"Journal of Biological Chemistry"},{"issue":"6","key":"pcbi.1008855.ref068","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1016\/j.molcel.2017.08.012","article-title":"Endoplasmic reticulum transport of glutathione by Sec61 is regulated by Ero1 and Bip","volume":"67","author":"AJ Ponsero","year":"2017","journal-title":"Molecular cell"},{"issue":"22","key":"pcbi.1008855.ref069","doi-asserted-by":"crossref","first-page":"15845","DOI":"10.1074\/jbc.M113.533794","article-title":"Structural and functional profiling of the lateral gate of the Sec61 translocon","volume":"289","author":"JH Reithinger","year":"2014","journal-title":"Journal of Biological Chemistry"},{"issue":"W1","key":"pcbi.1008855.ref070","doi-asserted-by":"crossref","first-page":"W368","DOI":"10.1093\/nar\/gky309","article-title":"MOLEonline: a web-based tool for analyzing channels, tunnels and pores (2018 update)","volume":"46","author":"L Pravda","year":"2018","journal-title":"Nucleic acids research"}],"updated-by":[{"DOI":"10.1371\/journal.pcbi.1008855","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,4,8]],"date-time":"2021-04-08T00:00:00Z","timestamp":1617840000000}}],"container-title":["PLOS Computational Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pcbi.1008855","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,8]],"date-time":"2021-04-08T20:55:01Z","timestamp":1617915301000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pcbi.1008855"}},"subtitle":[],"editor":[{"given":"Alexander","family":"MacKerell","sequence":"first","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2021,3,29]]},"references-count":70,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,3,29]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pcbi.1008855","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.pcbi.1008855","asserted-by":"object"}]},"ISSN":["1553-7358"],"issn-type":[{"value":"1553-7358","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,29]]}}}