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Distributed systems, however, introduce additional challenges since consortium composition and spatiotemporal dynamics of constituent strains must be robustly controlled to achieve desired circuit behaviors. Here, we address these challenges with a modeling-based investigation of emergent spatiotemporal population dynamics using cell-length control in monolayer, two-strain bacterial consortia. We demonstrate that with dynamic control of a strain\u2019s division length, nematic cell alignment in close-packed monolayers can be destabilized. We find that this destabilization confers an emergent, competitive advantage to smaller-length strains\u2014but by mechanisms that differ depending on the spatial patterns of the population. We used complementary modeling approaches to elucidate underlying mechanisms: an agent-based model to simulate detailed mechanical and signaling interactions between the competing strains, and a reductive, stochastic lattice model to represent cell-cell interactions with a single rotational parameter. Our modeling suggests that spatial strain-fraction oscillations can be generated when cell-length control is coupled to quorum-sensing signaling in negative feedback topologies. Our research employs novel methods of population control and points the way to programming strain fraction dynamics in consortial synthetic biology.<\/jats:p>","DOI":"10.1371\/journal.pcbi.1009381","type":"journal-article","created":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T14:40:35Z","timestamp":1632321635000},"page":"e1009381","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":26,"title":["Emergent spatiotemporal population dynamics with cell-length control of synthetic microbial consortia"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8863-3133","authenticated-orcid":true,"given":"James J.","family":"Winkle","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6926-7593","authenticated-orcid":true,"given":"Bhargav R.","family":"Karamched","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4975-8854","authenticated-orcid":true,"given":"Matthew R.","family":"Bennett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7119-491X","authenticated-orcid":true,"given":"William","family":"Ott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1975-3913","authenticated-orcid":true,"given":"Kre\u0161imir","family":"Josi\u0107","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,9,22]]},"reference":[{"key":"pcbi.1009381.ref001","doi-asserted-by":"crossref","DOI":"10.1038\/nmicrobiol.2017.83","article-title":"A stabilized microbial ecosystem of self-limiting bacteria using synthetic quorum-regulated lysis","volume":"2","author":"SR Scott","year":"2017","journal-title":"Nat Microbiol"},{"key":"pcbi.1009381.ref002","doi-asserted-by":"crossref","unstructured":"Bittihn P, Din MO, Tsimring LS, Hasty J. 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