{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:39:23Z","timestamp":1787243963227,"version":"build-2736575974"},"update-to":[{"DOI":"10.1371\/journal.pcbi.1009658","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T00:00:00Z","timestamp":1639958400000}}],"reference-count":89,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2021,12,8]],"date-time":"2021-12-08T00:00:00Z","timestamp":1638921600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000781","name":"european research council","doi-asserted-by":"publisher","award":["AdG788970"],"award-info":[{"award-number":["AdG788970"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001674","name":"fondation leducq","doi-asserted-by":"publisher","award":["11 CVD-01"],"award-info":[{"award-number":["11 CVD-01"]}],"id":[{"id":"10.13039\/501100001674","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"bundesministerium f\u00fcr bildung und forschung","doi-asserted-by":"publisher","award":["CaRNAtion"],"award-info":[{"award-number":["CaRNAtion"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.ploscompbiol.org"],"crossmark-restriction":false},"short-container-title":["PLoS Comput Biol"],"abstract":"<jats:p>\n                    RNA-binding proteins (RBPs) can regulate more than a single aspect of RNA metabolism. We searched for such previously undiscovered multifunctionality within a set of 143 RBPs, by defining the predictive value of RBP abundance for the transcription and translation levels of known RBP target genes across 80 human hearts. This led us to newly associate 27 RBPs with cardiac translational regulation\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Of these, 21 impacted both RNA expression and translation, albeit for virtually independent sets of target genes. We highlight a subset of these, including G3BP1, PUM1, UCHL5, and DDX3X, where dual regulation is achieved through differential affinity for target length, by which separate biological processes are controlled. Like the RNA helicase DDX3X, the known splicing factors EFTUD2 and PRPF8\u2014all identified as multifunctional RBPs by our analysis\u2014selectively influence target translation rates depending on 5\u2019 UTR structure. Our analyses identify dozens of RBPs as being multifunctional and pinpoint potential novel regulators of translation, postulating unanticipated complexity of protein-RNA interactions at consecutive stages of gene expression.\n                  <\/jats:p>","DOI":"10.1371\/journal.pcbi.1009658","type":"journal-article","created":{"date-parts":[[2021,12,8]],"date-time":"2021-12-08T13:44:06Z","timestamp":1638971046000},"page":"e1009658","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":42,"title":["Multifunctional RNA-binding proteins influence mRNA abundance and translational efficiency of distinct sets of target genes"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3980-3789","authenticated-orcid":true,"given":"Valentin","family":"Schneider-Lunitz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Ruiz-Orera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1218-6223","authenticated-orcid":true,"given":"Norbert","family":"Hubner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9593-1980","authenticated-orcid":true,"given":"Sebastiaan","family":"van Heesch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,12,8]]},"reference":[{"key":"pcbi.1009658.ref001","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1038\/nrg3813","article-title":"A census of human RNA-binding proteins","volume":"15","author":"S Gerstberger","year":"2014","journal-title":"Nat Rev Genet"},{"key":"pcbi.1009658.ref002","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.molcel.2019.04.018","article-title":"R-DeeP: Proteome-wide and Quantitative Identification of RNA-Dependent Proteins by Density Gradient Ultracentrifugation","volume":"75","author":"M Caudron-Herger","year":"2019","journal-title":"Mol Cell"},{"key":"pcbi.1009658.ref003","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1016\/j.celrep.2019.09.060","article-title":"Systematic Discovery of Endogenous Human Ribonucleoprotein Complexes","volume":"29","author":"AL Mallam","year":"2019","journal-title":"Cell Rep"},{"key":"pcbi.1009658.ref004","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.cell.2018.11.004","article-title":"The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest","volume":"176","author":"J Trendel","year":"2019","journal-title":"Cell"},{"key":"pcbi.1009658.ref005","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1016\/j.molcel.2016.06.029","article-title":"Comprehensive Identification of RNA-Binding Domains in Human Cells","volume":"63","author":"A Castello","year":"2016","journal-title":"Mol Cell"},{"key":"pcbi.1009658.ref006","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1007\/s00424-016-1819-4","article-title":"The expanding universe of ribonucleoproteins: of novel RNA-binding proteins and unconventional interactions","volume":"468","author":"BM Beckmann","year":"2016","journal-title":"Pflugers Arch"},{"key":"pcbi.1009658.ref007","article-title":"The cohesin loader NIPBL interacts with pre-ribosomal RNA and treacle to regulate ribosomal RNA synthesis","author":"X Kong","year":"2019","journal-title":"bioRxiv"},{"key":"pcbi.1009658.ref008","doi-asserted-by":"crossref","first-page":"67","DOI":"10.3389\/fmolb.2017.00067","article-title":"Handshakes and Fights: The Regulatory Interplay of RNA-Binding Proteins.","volume":"4","author":"E Dassi","year":"2017","journal-title":"Front Mol Biosci"},{"key":"pcbi.1009658.ref009","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.3389\/fimmu.2018.01094","article-title":"Uncovering the Role of RNA-Binding Proteins in Gene Expression in the Immune System.","volume":"9","author":"MD D\u00edaz-Mu\u00f1oz","year":"2018","journal-title":"Front Immunol"},{"key":"pcbi.1009658.ref010","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1042\/BST20150171","article-title":"Poly(A)-binding proteins and mRNA localization: who rules the roost?","volume":"43","author":"NK Gray","year":"2015","journal-title":"Biochem Soc Trans"},{"key":"pcbi.1009658.ref011","doi-asserted-by":"crossref","first-page":"e0231450","DOI":"10.1371\/journal.pone.0231450","article-title":"Analysis of the nucleocytoplasmic shuttling RNA-binding protein HNRNPU using optimized HITS-CLIP method.","volume":"15","author":"M Yugami","year":"2020","journal-title":"PLoS One."},{"key":"pcbi.1009658.ref012","first-page":"711","article-title":"A large-scale binding and functional map of human RNA-binding proteins","volume":"583","author":"EL Van Nostrand","year":"2020","journal-title":"Nature. 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