{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T06:16:05Z","timestamp":1772172965381,"version":"3.50.1"},"update-to":[{"DOI":"10.1371\/journal.pcbi.1009779","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T00:00:00Z","timestamp":1643241600000}}],"reference-count":93,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2022,1,14]],"date-time":"2022-01-14T00:00:00Z","timestamp":1642118400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1615916"],"award-info":[{"award-number":["1615916"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.ploscompbiol.org"],"crossmark-restriction":false},"short-container-title":["PLoS Comput Biol"],"abstract":"<jats:p>Cellular differentiation during hematopoiesis is guided by gene regulatory networks (GRNs) comprising transcription factors (TFs) and the effectors of cytokine signaling. Based largely on analyses conducted at steady state, these GRNs are thought to be organized as a hierarchy of bistable switches, with antagonism between Gata1 and PU.1 driving red- and white-blood cell differentiation. Here, we utilize transient gene expression patterns to infer the genetic architecture\u2014the type and strength of regulatory interconnections\u2014and dynamics of a twelve-gene GRN including key TFs and cytokine receptors. We trained gene circuits, dynamical models that learn genetic architecture, on high temporal-resolution gene-expression data from the differentiation of an inducible cell line into erythrocytes and neutrophils. The model is able to predict the consequences of gene knockout, knockdown, and overexpression experiments and the inferred interconnections are largely consistent with prior empirical evidence. The inferred genetic architecture is densely interconnected rather than hierarchical, featuring extensive cross-antagonism between genes from alternative lineages and positive feedback from cytokine receptors. The analysis of the dynamics of gene regulation in the model reveals that PU.1 is one of the last genes to be upregulated in neutrophil conditions and that the upregulation of PU.1 and other neutrophil genes is driven by Cebpa and Gfi1 instead. This model inference is confirmed in an independent single-cell RNA-Seq dataset from mouse bone marrow in which Cebpa and Gfi1 expression precedes the neutrophil-specific upregulation of PU.1 during differentiation. These results demonstrate that full PU.1 upregulation during neutrophil development involves regulatory influences extrinsic to the Gata1-PU.1 bistable switch. Furthermore, although there is extensive cross-antagonism between erythroid and neutrophil genes, it does not have a hierarchical structure. More generally, we show that the combination of high-resolution time series data and data-driven dynamical modeling can uncover the dynamics and causality of developmental events that might otherwise be obscured.<\/jats:p>","DOI":"10.1371\/journal.pcbi.1009779","type":"journal-article","created":{"date-parts":[[2022,1,14]],"date-time":"2022-01-14T14:43:09Z","timestamp":1642171389000},"page":"e1009779","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Data-driven modeling predicts gene regulatory network dynamics during the differentiation of multipotential hematopoietic progenitors"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8679-7046","authenticated-orcid":true,"given":"Joanna E.","family":"Handzlik","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7677-7228","authenticated-orcid":true,"family":"Manu","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2022,1,14]]},"reference":[{"issue":"4","key":"pcbi.1009779.ref001","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.cell.2008.01.025","article-title":"Hematopoiesis: an evolving paradigm for stem cell biology","volume":"132","author":"SH Orkin","year":"2008","journal-title":"Cell"},{"issue":"4","key":"pcbi.1009779.ref002","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.smim.2008.08.003","article-title":"Gene regulatory networks directing myeloid and lymphoid cell fates within the immune system","volume":"20","author":"P Laslo","year":"2008","journal-title":"Semin 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