{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T17:23:12Z","timestamp":1775236992910,"version":"3.50.1"},"reference-count":160,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2022,5,13]],"date-time":"2022-05-13T00:00:00Z","timestamp":1652400000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["U01CA202958"],"award-info":[{"award-number":["U01CA202958"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["U01CA244101"],"award-info":[{"award-number":["U01CA244101"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["U01CA244101"],"award-info":[{"award-number":["U01CA244101"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["U01CA202958"],"award-info":[{"award-number":["U01CA202958"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.ploscompbiol.org"],"crossmark-restriction":false},"short-container-title":["PLoS Comput Biol"],"abstract":"<jats:p>\n                    Myeloid-derived monocyte and macrophages are key cells in the bone that contribute to remodeling and injury repair. However, their temporal polarization status and control of bone-resorbing osteoclasts and bone-forming osteoblasts responses is largely unknown. In this study, we focused on two aspects of monocyte\/macrophage dynamics and polarization states over time: 1) the injury-triggered pro- and anti-inflammatory monocytes\/macrophages temporal profiles, 2) the contributions of pro- versus anti-inflammatory monocytes\/macrophages in coordinating healing response. Bone healing is a complex multicellular dynamic process. While traditional\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    experimentation may capture the behavior of select populations with high resolution, they cannot simultaneously track the behavior of multiple populations. To address this, we have used an integrated coupled ordinary differential equations (ODEs)-based framework describing multiple cellular species to\n                    <jats:italic>in vivo<\/jats:italic>\n                    bone injury data in order to identify and test various hypotheses regarding bone cell populations dynamics. Our approach allowed us to infer several biological insights including, but not limited to,: 1) anti-inflammatory macrophages are key for early osteoclast inhibition and pro-inflammatory macrophage suppression, 2) pro-inflammatory macrophages are involved in osteoclast bone resorptive activity, whereas osteoblasts promote osteoclast differentiation, 3) Pro-inflammatory monocytes\/macrophages rise during two expansion waves, which can be explained by the anti-inflammatory macrophages-mediated inhibition phase between the two waves. In addition, we further tested the robustness of the mathematical model by comparing simulation results to an independent experimental dataset. Taken together, this novel comprehensive mathematical framework allowed us to identify biological mechanisms that best recapitulate bone injury data and that explain the coupled cellular population dynamics involved in the process. Furthermore, our hypothesis testing methodology could be used in other contexts to decipher mechanisms in complex multicellular processes.\n                  <\/jats:p>","DOI":"10.1371\/journal.pcbi.1009839","type":"journal-article","created":{"date-parts":[[2022,5,13]],"date-time":"2022-05-13T13:40:23Z","timestamp":1652449223000},"page":"e1009839","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Integrated computational and in vivo models reveal Key Insights into macrophage behavior during bone healing"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6944-3569","authenticated-orcid":true,"given":"Etienne","family":"Baratchart","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8848-2507","authenticated-orcid":true,"given":"Chen Hao","family":"Lo","sequence":"additional","affiliation":[]},{"given":"Conor C.","family":"Lynch","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8527-0776","authenticated-orcid":true,"given":"David","family":"Basanta","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2022,5,13]]},"reference":[{"key":"pcbi.1009839.ref001","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/j.semcdb.2008.07.004","article-title":"Bone remodeling during fracture repair: The cellular picture","volume":"19","author":"A. 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