{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T06:53:38Z","timestamp":1768892018727,"version":"3.49.0"},"update-to":[{"DOI":"10.1371\/journal.pcbi.1011594","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,11,9]],"date-time":"2023-11-09T00:00:00Z","timestamp":1699488000000}}],"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T00:00:00Z","timestamp":1698624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI167860"],"award-info":[{"award-number":["R01AI167860"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.ploscompbiol.org"],"crossmark-restriction":false},"short-container-title":["PLoS Comput Biol"],"abstract":"<jats:p><jats:italic>Bacteroides fragilis<\/jats:italic> is a universal member of the dominant commensal gut phylum Bacteroidetes. Its fermentation products and abundance have been linked to obesity, inflammatory bowel disease, and other disorders through its effects on host metabolic regulation and the immune system. As of yet, there has been no curated systems-level characterization of <jats:italic>B<\/jats:italic>. <jats:italic>fragilis<\/jats:italic>\u2019 metabolism that provides a comprehensive analysis of the link between human diet and <jats:italic>B<\/jats:italic>. <jats:italic>fragilis<\/jats:italic>\u2019 metabolic products. To address this, we developed a genome-scale metabolic model of <jats:italic>B<\/jats:italic>. <jats:italic>fragilis<\/jats:italic> strain 638R. The model <jats:italic>i<\/jats:italic>MN674 contains 1,634 reactions, 1,362 metabolites, three compartments, and reflects the strain\u2019s ability to utilize 142 metabolites. Predictions made with this model include its growth rate and efficiency on these substrates, the amounts of each fermentation product it produces under different conditions, and gene essentiality for each biomass component. The model highlights and resolves gaps in knowledge of <jats:italic>B<\/jats:italic>. <jats:italic>fragilis<\/jats:italic>\u2019 carbohydrate metabolism and its corresponding transport proteins. This high quality model provides the basis for rational prediction of <jats:italic>B<\/jats:italic>. <jats:italic>fragilis<\/jats:italic>\u2019 metabolic interactions with its environment and its host.<\/jats:p>","DOI":"10.1371\/journal.pcbi.1011594","type":"journal-article","created":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T18:07:45Z","timestamp":1698689265000},"page":"e1011594","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Genome-scale metabolic modeling of the human gut bacterium Bacteroides fragilis strain 638R"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6284-3656","authenticated-orcid":true,"given":"Maxwell","family":"Neal","sequence":"first","affiliation":[]},{"given":"Deepan","family":"Thiruppathy","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8062-3296","authenticated-orcid":true,"given":"Karsten","family":"Zengler","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2023,10,30]]},"reference":[{"key":"pcbi.1011594.ref001","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1128\/CMR.00008-07","article-title":"Bacteroides: the good, the bad, and the nitty-gritty","volume":"20","author":"HM Wexler","year":"2007","journal-title":"Clin Microbiol Rev"},{"key":"pcbi.1011594.ref002","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1038\/nature02285","article-title":"The strict anaerobe Bacteroides fragilis grows in and benefits from nanomolar concentrations of oxygen","volume":"427","author":"AD Baughn","year":"2004","journal-title":"Nature"},{"key":"pcbi.1011594.ref003","doi-asserted-by":"crossref","first-page":"14","DOI":"10.3390\/microorganisms7010014","article-title":"What is the healthy gut microbiota composition? 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