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Here, we introduce MEDUSA (\n                    <jats:bold>ME<\/jats:bold>\n                    mbrane\n                    <jats:bold>D<\/jats:bold>\n                    iff\n                    <jats:bold>U<\/jats:bold>\n                    se\n                    <jats:bold>S<\/jats:bold>\n                    cattering\n                    <jats:bold>A<\/jats:bold>\n                    nalysis), a cloud-based analysis tool to determine the bending modulus,\n                    <jats:italic>\u03ba<\/jats:italic>\n                    , from the analysis of X-ray diffuse scattering. MEDUSA uses GPU (graphics processing unit) accelerated hardware and a parallelized algorithm to run the calculations efficiently in a few seconds. MEDUSA\u2019s graphical user interface allows the user to upload 2-dimensional data collected from different sources, perform background subtraction and distortion corrections, select regions of interest, run the fitting procedure and output the fitted parameters, the membranes\u2019 bending modulus\n                    <jats:italic>\u03ba<\/jats:italic>\n                    , and compressional modulus\n                    <jats:italic>B<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1371\/journal.pcbi.1011749","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T13:32:11Z","timestamp":1704720731000},"page":"e1011749","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["MEDUSA: A cloud-based tool for the analysis of X-ray diffuse scattering to obtain the bending modulus from oriented membrane stacks"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8193-5654","authenticated-orcid":true,"given":"Sebastian","family":"Himbert","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorian","family":"Gaboo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emre","family":"Brookes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John F.","family":"Nagle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0558-7475","authenticated-orcid":true,"given":"Maikel C.","family":"Rheinst\u00e4dter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,1,8]]},"reference":[{"key":"pcbi.1011749.ref001","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1038\/ncb2570","article-title":"Bending membranes","volume":"14","author":"T Kirchhausen","year":"2012","journal-title":"Nature Cell Biology"},{"key":"pcbi.1011749.ref002","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1038\/nature04396","article-title":"Membrane curvature and mechanisms of dynamic cell membrane remodelling","volume":"438","author":"H.T. 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