{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T12:48:41Z","timestamp":1776948521734,"version":"3.51.4"},"update-to":[{"DOI":"10.1371\/journal.pcbi.1012449","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T00:00:00Z","timestamp":1728864000000}}],"reference-count":34,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2024,10,2]],"date-time":"2024-10-02T00:00:00Z","timestamp":1727827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100001246","name":"Ambrose Monell Foundation","doi-asserted-by":"publisher","award":["Switch grant"],"award-info":[{"award-number":["Switch grant"]}],"id":[{"id":"10.13039\/100001246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001006","name":"Breast Cancer Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001006","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NICHD\/NIH","award":["R01HD105049"],"award-info":[{"award-number":["R01HD105049"]}]}],"content-domain":{"domain":["www.ploscompbiol.org"],"crossmark-restriction":false},"short-container-title":["PLoS Comput Biol"],"abstract":"<jats:p>Persons with germline variants in the tumor suppressor gene phosphatase and tensin homolog, <jats:italic>PTEN<\/jats:italic>, are molecularly diagnosed with PTEN hamartoma tumor syndrome (PHTS). PHTS confers high risks of specific malignancies, and up to 23% of the patients are diagnosed with autism spectrum disorder (ASD) and\/or developmental delay (DD). The accurate prediction of these two seemingly disparate phenotypes (cancer <jats:italic>vs<\/jats:italic>. ASD\/DD) for PHTS at the individual level remains elusive despite the available statistical prevalence of specific phenotypes of the syndrome at the population level. The pleiotropy of the syndrome may, in part, be due to the alterations of the key multi-functions of PTEN. Maintenance of genome integrity is one of the key biological functions of PTEN, but no integrative studies have been conducted to quantify the DNA damage response (DDR) in individuals with PHTS and to relate to phenotypes and genotypes. In this study, we used 43 PHTS patient-derived lymphoblastoid cell lines (LCLs) to investigate the associations between DDR and <jats:italic>PTEN<\/jats:italic> genotypes and\/or clinical phenotypes ASD\/DD <jats:italic>vs<\/jats:italic>. cancer. The dynamics of DDR of \u03b3-irradiated LCLs were analyzed using the exponential decay mathematical model to fit temporal changes in \u03b3H2AX levels which report the degree of DNA damage. We found that <jats:italic>PTEN<\/jats:italic> nonsense variants are associated with less efficient DNA damage repair ability resulting in higher DNA damage levels at 24 hours after irradiation compared to <jats:italic>PTEN<\/jats:italic> missense variants. Regarding PHTS phenotypes, LCLs from PHTS individuals with ASD\/DD showed faster DNA damage repairing rate than those from patients without ASD\/DD or cancer. We also applied the reaction-diffusion partial differential equation (PDE) mathematical model, a cell growth model with a DNA damage term, to accurately describe the DDR process in the LCLs. For each LCL, we can derive parameters of the PDE. Then we averaged the numerical results by PHTS phenotypes. By performing simple subtraction of two subgroup average results, we found that PHTS-ASD\/DD is associated with higher live cell density at lower DNA damage level but lower cell density level at higher DNA damage level compared to LCLs from individuals with PHTS-cancer and PHTS-neither.<\/jats:p>","DOI":"10.1371\/journal.pcbi.1012449","type":"journal-article","created":{"date-parts":[[2024,10,2]],"date-time":"2024-10-02T17:32:57Z","timestamp":1727890377000},"page":"e1012449","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Quantitative evaluation of DNA damage repair dynamics to elucidate predictors of autism vs. cancer in individuals with germline PTEN variants"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6056-222X","authenticated-orcid":true,"given":"Ruipeng","family":"Wei","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Hitomi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tammy","family":"Sadler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lamis","family":"Yehia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Calvetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2971-7673","authenticated-orcid":true,"given":"Jacob","family":"Scott","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charis","family":"Eng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2024,10,2]]},"reference":[{"issue":"6144","key":"pcbi.1012449.ref001","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1126\/science.1236188","article-title":"Nuclear 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