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Interpretation of stable isotope labelling data (as for BrdU and CFSE) necessitates simplifying assumptions. Here we investigate the impact of three of the most commonly used simplifying assumptions: (i) that the cell population of interest is closed, (ii) that the population of interest is kinetically homogeneous, and (iii) that the population is spatially homogeneous and suggest pragmatic ways in which the resulting errors can be reduced.\n                  <\/jats:p>","DOI":"10.1371\/journal.pcbi.1012704","type":"journal-article","created":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T16:14:29Z","timestamp":1748967269000},"page":"e1012704","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["The impact of model assumptions in interpreting cell kinetic studies"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7456-339X","authenticated-orcid":true,"given":"Ada Wing 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