{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T04:59:49Z","timestamp":1778734789019,"version":"3.51.4"},"reference-count":25,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T00:00:00Z","timestamp":1745193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100001229","name":"G. Harold and Leila Y. Mathers Charitable Foundation","doi-asserted-by":"publisher","award":["MF-2107-01851"],"award-info":[{"award-number":["MF-2107-01851"]}],"id":[{"id":"10.13039\/100001229","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NIH","award":["R01AI175245, T32AI112541"],"award-info":[{"award-number":["R01AI175245, T32AI112541"]}]},{"DOI":"10.13039\/100023015","name":"Advanced Research Projects Agency for Health","doi-asserted-by":"crossref","award":["1AY2AX000077"],"award-info":[{"award-number":["1AY2AX000077"]}],"id":[{"id":"10.13039\/100023015","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.ploscompbiol.org"],"crossmark-restriction":false},"short-container-title":["PLoS Comput Biol"],"abstract":"<jats:p>The potential diversity in the global repertoire of human antibody sequences is currently not well understood due to the limited existing paired antibody heavy-light chain sequence data that has been hindered by the low throughput and high costs of current single-cell sequencing methods. Here, we report IgHuAb, a large language model for high-throughput generation of paired human antibody sequences. Using IgHuAb, we created SynAbLib, a synthetic human antibody library that mimics population-level features of naturally occurring human antibody sequences, yet is associated with significantly greater diversity in sequence space. Further, experimental validation of a diverse set of antibodies from SynAbLib showed robust expression yields. IgHuAb and SynAbLib provide a readily expandable platform for human monoclonal antibody generation that can be efficiently mined for antibody sequences with target properties.<\/jats:p>","DOI":"10.1371\/journal.pcbi.1012932","type":"journal-article","created":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T17:48:58Z","timestamp":1745257738000},"page":"e1012932","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["An expandable synthetic library of human paired antibody sequences"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-7029-2641","authenticated-orcid":true,"given":"Toma M.","family":"Marinov","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Perry 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Kenlay, F.A. Dreyer, A. Kovaltsuk, D. Miketa, D. Pires, C.M Deane. Large scale paired antibody language models. 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