{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T11:09:29Z","timestamp":1774091369172,"version":"3.50.1"},"update-to":[{"DOI":"10.1371\/journal.pcbi.1012992","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T00:00:00Z","timestamp":1745798400000}}],"reference-count":20,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T00:00:00Z","timestamp":1744761600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP22H04926"],"award-info":[{"award-number":["JP22H04926"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.ploscompbiol.org"],"crossmark-restriction":false},"short-container-title":["PLoS Comput Biol"],"abstract":"<jats:p>RMPJ is an image-processing tool that facilitates the effective enhancement of low-contrast analysis objects and enables preprocessing to quantify the morphological features of the objects under analysis. This ImageJ plugin implements seven image-processing algorithms based on rotational morphological processing (RMP), a variation of traditional morphological operations. RMPJ enables the enhancement and extraction of intricate and nuanced features in biomedical images. Furthermore, it facilitates the separation and extraction of multiple aggregated structures. This software aims to expand the scope of research in life sciences by providing an image-processing method that quantitatively analyzes the morphological information of objects.<\/jats:p>","DOI":"10.1371\/journal.pcbi.1012992","type":"journal-article","created":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T17:49:01Z","timestamp":1744825741000},"page":"e1012992","update-policy":"https:\/\/doi.org\/10.1371\/journal.pcbi.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["RMPJ: An ImageJ plugin for morphological information processing in biomedical images"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7931-8083","authenticated-orcid":true,"given":"Yoshitaka","family":"Kimori","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,4,16]]},"reference":[{"key":"pcbi.1012992.ref001","volume-title":"Mathematical morphology: from theory to applications","author":"L Najman","year":"2010"},{"key":"pcbi.1012992.ref002","volume-title":"Mathematical morphology in image processing","author":"ER Dougherty","year":"1993"},{"key":"pcbi.1012992.ref003","volume-title":"Morphological image analysis","author":"P Soille","year":"2003"},{"issue":"1","key":"pcbi.1012992.ref004","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jsb.2011.07.007","article-title":"Fractal dimension analysis and mathematical morphology of structural changes in actin filaments imaged by electron microscopy","volume":"176","author":"Y Kimori","year":"2011","journal-title":"J Struct Biol"},{"key":"pcbi.1012992.ref005","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1186\/1471-2105-11-373","article-title":"Extended morphological processing: a practical method for automatic spot detection of biological markers from microscopic images","volume":"11","author":"Y Kimori","year":"2010","journal-title":"BMC Bioinformatics"},{"key":"pcbi.1012992.ref006","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.jtbi.2015.10.031","article-title":"Quantifying morphological features of actin cytoskeletal filaments in plant cells based on mathematical morphology","volume":"389","author":"Y Kimori","year":"2016","journal-title":"J Theor Biol"},{"key":"pcbi.1012992.ref007","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/2043-9113-1-33","article-title":"Mathematical morphology-based approach to the enhancement of morphological features in medical images","volume":"1","author":"Y Kimori","year":"2011","journal-title":"J Clin Bioinforma"},{"key":"pcbi.1012992.ref008","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1107\/S0909049513020761","article-title":"Morphological image processing for quantitative shape analysis of biomedical structures: effective contrast enhancement","volume":"20","author":"Y Kimori","year":"2013","journal-title":"J Synchrotron Radiat"},{"issue":"7","key":"pcbi.1012992.ref009","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"CA Schneider","year":"2012","journal-title":"Nature Methods"},{"issue":"22","key":"pcbi.1012992.ref010","doi-asserted-by":"crossref","first-page":"3532","DOI":"10.1093\/bioinformatics\/btw413","article-title":"MorphoLibJ: integrated library and plugins for mathematical morphology with ImageJ","volume":"32","author":"D Legland","year":"2016","journal-title":"Bioinformatics"},{"issue":"1","key":"pcbi.1012992.ref011","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1109\/TSMC.1979.4310076","article-title":"A threshold selection method from gray-level histograms","volume":"9","author":"N Otsu","year":"1979","journal-title":"IEEE Trans on Syst Man Cybern"},{"key":"pcbi.1012992.ref012","article-title":"Mammographic image analysis society (MIAS) database v1.21","author":"J Suckling","year":"2015"},{"issue":"7","key":"pcbi.1012992.ref013","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1038\/nmeth.2083","article-title":"Annotated high-throughput microscopy image sets for validation","volume":"9","author":"V Ljosa","year":"2012","journal-title":"Nat Methods"},{"issue":"3","key":"pcbi.1012992.ref014","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/0734-189X(85)90133-1","article-title":"Moment-preserving thresholding: A new approach","volume":"29","author":"W-H Tsai","year":"1985","journal-title":"Comput Vis Graph Image Process"},{"issue":"13","key":"pcbi.1012992.ref015","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera\u2014a visualization system for exploratory research and analysis","volume":"25","author":"EF Pettersen","year":"2004","journal-title":"J Comput Chem"},{"key":"pcbi.1012992.ref016","doi-asserted-by":"crossref","first-page":"70919","DOI":"10.1109\/ACCESS.2022.3187507","article-title":"A Morphological Image Preprocessing Method Based on the Geometrical Shape of Lesions to Improve the Lesion Recognition Performance of Convolutional Neural Networks","volume":"10","author":"Y Kimori","year":"2022","journal-title":"IEEE Access"},{"issue":"11","key":"pcbi.1012992.ref017","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1109\/34.969120","article-title":"Directional morphological filtering","volume":"23","author":"P Soille","year":"2001","journal-title":"IEEE Trans Pattern Anal Machine Intell"},{"issue":"4","key":"pcbi.1012992.ref018","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/j.imavis.2006.07.021","article-title":"Efficient complete and incomplete path openings and closings","volume":"25","author":"H Talbot","year":"2007","journal-title":"Image Vision Comput"},{"issue":"2","key":"pcbi.1012992.ref019","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1109\/TPAMI.2017.2672972","article-title":"Curvilinear structure analysis by ranking the orientation responses of path operators","volume":"40","author":"O Merveille","year":"2018","journal-title":"IEEE Trans Pattern Anal Mach Intell"},{"issue":"7","key":"pcbi.1012992.ref020","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1109\/34.192465","article-title":"Pattern spectrum and multiscale shape representation","volume":"11","author":"P Maragos","year":"1989","journal-title":"IEEE Trans Pattern Anal Mach Intell"}],"updated-by":[{"DOI":"10.1371\/journal.pcbi.1012992","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T00:00:00Z","timestamp":1745798400000}}],"container-title":["PLOS Computational Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pcbi.1012992","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T17:56:23Z","timestamp":1745862983000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pcbi.1012992"}},"subtitle":[],"editor":[{"given":"Stacey D.","family":"Finley","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2025,4,16]]},"references-count":20,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,4,16]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pcbi.1012992","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.pcbi.1012992","asserted-by":"object"}]},"ISSN":["1553-7358"],"issn-type":[{"value":"1553-7358","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,16]]}}}