{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T16:19:40Z","timestamp":1792081180695,"version":"4.4.0"},"update-to":[{"DOI":"10.1371\/journal.pbio.3000943","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2020,11,30]],"date-time":"2020-11-30T00:00:00Z","timestamp":1606694400000}}],"reference-count":59,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T00:00:00Z","timestamp":1605484800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Hi1414\/6-1"],"award-info":[{"award-number":["Hi1414\/6-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["WE2720\/4-1"],"award-info":[{"award-number":["WE2720\/4-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["MO1084\/2-1"],"award-info":[{"award-number":["MO1084\/2-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["AxoGlia"],"award-info":[{"award-number":["AxoGlia"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["MyeliNANO"],"award-info":[{"award-number":["MyeliNANO"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Research Center Nanoscale Microscopy and Molecular Physiology of the Brain"],"award-info":[{"award-number":["Research Center Nanoscale Microscopy and Molecular Physiology of the Brain"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Research Center Nanoscale Microscopy and Molecular Physiology of the Brain"],"award-info":[{"award-number":["Research Center Nanoscale Microscopy and Molecular Physiology of the Brain"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB-TR43"],"award-info":[{"award-number":["SFB-TR43"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Na262\/1-2"],"award-info":[{"award-number":["Na262\/1-2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosbiology.org"],"crossmark-restriction":false},"short-container-title":["PLoS Biol"],"abstract":"<jats:p>In several neurodegenerative disorders, axonal pathology may originate from impaired oligodendrocyte-to-axon support of energy substrates. We previously established transgenic mice that allow measuring axonal ATP levels in electrically active optic nerves. Here, we utilize this technique to explore axonal ATP dynamics in the <jats:italic>Plp<\/jats:italic><jats:sup>null\/y<\/jats:sup> mouse model of spastic paraplegia. Optic nerves from <jats:italic>Plp<\/jats:italic><jats:sup>null\/y<\/jats:sup> mice exhibited lower and more variable basal axonal ATP levels and reduced compound action potential (CAP) amplitudes, providing a missing link between axonal pathology and a role of oligodendrocytes in brain energy metabolism. Surprisingly, when <jats:italic>Plp<\/jats:italic><jats:sup>null\/y<\/jats:sup> optic nerves are challenged with transient glucose deprivation, both ATP levels and CAP decline slower, but recover faster upon reperfusion of glucose. Structurally, myelin sheaths display an increased frequency of cytosolic channels comprising glucose and monocarboxylate transporters, possibly facilitating accessibility of energy substrates to the axon. These data imply that complex metabolic alterations of the axon\u2013myelin unit contribute to the phenotype of <jats:italic>Plp<\/jats:italic><jats:sup>null\/y<\/jats:sup> mice.<\/jats:p>","DOI":"10.1371\/journal.pbio.3000943","type":"journal-article","created":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T13:35:44Z","timestamp":1605533744000},"page":"e3000943","update-policy":"https:\/\/doi.org\/10.1371\/journal.pbio.corrections_policy","source":"Crossref","is-referenced-by-count":38,"title":["Structural myelin defects are associated with low axonal ATP levels but rapid recovery from energy deprivation in a mouse model of spastic paraplegia"],"prefix":"10.1371","volume":"18","author":[{"given":"Andrea","family":"Trevisiol","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5079-502X","authenticated-orcid":true,"given":"Kathrin","family":"Kusch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4814-7517","authenticated-orcid":true,"given":"Anna M.","family":"Steyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1775-2159","authenticated-orcid":true,"given":"Ingo","family":"Gregor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christos","family":"Nardis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrike","family":"Winkler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"K\u00f6hler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Restrepo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2902-7165","authenticated-orcid":true,"given":"Wiebke","family":"M\u00f6bius","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7710-5738","authenticated-orcid":true,"given":"Hauke B.","family":"Werner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus-Armin","family":"Nave","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6327-0089","authenticated-orcid":true,"given":"Johannes","family":"Hirrlinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,11,16]]},"reference":[{"issue":"29","key":"pbio.3000943.ref001","doi-asserted-by":"crossref","first-page":"6586","DOI":"10.1523\/JNEUROSCI.1034-18.2018","article-title":"Axonal ensheathment in the nervous system of lamprey: implications for the evolution of myelinating glia","volume":"38","author":"M-T Weil","year":"2018","journal-title":"J Neurosci"},{"key":"pbio.3000943.ref002","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1146\/annurev-cellbio-100913-013101","article-title":"Myelination of the nervous system: mechanisms and functions","volume":"30","author":"K-A Nave","year":"2014","journal-title":"Annu Rev Cell Dev Biol"},{"issue":"4","key":"pbio.3000943.ref003","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1038\/nrn2797","article-title":"Myelination and the trophic support of long axons","volume":"11","author":"K-A Nave","year":"2010","journal-title":"Nat Rev Neurosci."},{"issue":"7399","key":"pbio.3000943.ref004","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1038\/nature11007","article-title":"Glycolytic oligodendrocytes maintain myelin and long-term axonal integrity","volume":"485","author":"U F\u00fcnfschilling","year":"2012","journal-title":"Nature"},{"issue":"7408","key":"pbio.3000943.ref005","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1038\/nature11314","article-title":"Oligodendroglia metabolically support axons and contribute to neurodegeneration","volume":"487","author":"Y Lee","year":"2012","journal-title":"Nature"},{"issue":"11","key":"pbio.3000943.ref006","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1002\/glia.22737","article-title":"Adapting brain metabolism to myelination and long-range signal transduction","volume":"62","author":"J Hirrlinger","year":"2014","journal-title":"Glia"},{"issue":"1","key":"pbio.3000943.ref007","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.neuron.2016.05.016","article-title":"Oligodendroglial NMDA receptors regulate glucose import and axonal energy metabolism","volume":"91","author":"AS Saab","year":"2016","journal-title":"Neuron"},{"key":"pbio.3000943.ref008","doi-asserted-by":"crossref","first-page":"e24241","DOI":"10.7554\/eLife.24241","article-title":"Monitoring ATP dynamics in electrically active white matter tracts","volume":"6","author":"A Trevisiol","year":"2017","journal-title":"Elife"},{"key":"pbio.3000943.ref009","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.conb.2018.04.025","article-title":"Converging cellular themes for the hereditary spastic paraplegias","volume":"51","author":"C Blackstone","year":"2018","journal-title":"Curr Opin Neurobiol"},{"issue":"2","key":"pbio.3000943.ref010","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1017\/S1740925X0900009X","article-title":"Phylogeny of proteolipid proteins: divergence, constraints, and the evolution of novel functions in myelination and neuroprotection.","volume":"4","author":"W M\u00f6bius","year":"2008","journal-title":"Neuron Glia Biol"},{"issue":"1","key":"pbio.3000943.ref011","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/s12035-009-8071-2","article-title":"Myelin proteomics: molecular anatomy of an insulating sheath","volume":"40","author":"O Jahn","year":"2009","journal-title":"Mol Neurobiol"},{"issue":"4","key":"pbio.3000943.ref012","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1002\/glia.22456","article-title":"A critical role for the cholesterol-associated proteolipids PLP and M6B in myelination of the central nervous system","volume":"61","author":"HB Werner","year":"2013","journal-title":"Glia"},{"issue":"3","key":"pbio.3000943.ref013","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1038\/ng0394-257","article-title":"X-linked spastic paraplegia and Pelizaeus-Merzbacher disease are allelic disorders at the proteolipid protein locus","volume":"6","author":"P Saugier-Veber","year":"1994","journal-title":"Nat Genet"},{"issue":"Pt 3","key":"pbio.3000943.ref014","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1093\/brain\/awf043","article-title":"Patients lacking the major CNS myelin protein, proteolipid protein 1, develop length-dependent axonal degeneration in the absence of demyelination and inflammation","volume":"125","author":"JY Garbern","year":"2002","journal-title":"Brain"},{"issue":"2","key":"pbio.3000943.ref015","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/j.neuroscience.2009.12.047","article-title":"Novel neuronal proteolipid protein isoforms encoded by the human myelin proteolipid protein 1 gene","volume":"166","author":"C Sarret","year":"2010","journal-title":"Neuroscience"},{"issue":"11","key":"pbio.3000943.ref016","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.1002\/glia.23193","article-title":"Genetic dissection of oligodendroglial and neuronal Plp1 function in a novel mouse model of spastic paraplegia type 2","volume":"65","author":"KA L\u00fcders","year":"2017","journal-title":"Glia"},{"issue":"1","key":"pbio.3000943.ref017","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S0896-6273(01)80046-5","article-title":"Assembly of CNS myelin in the absence of proteolipid protein","volume":"18","author":"M Klugmann","year":"1997","journal-title":"Neuron"},{"issue":"5369","key":"pbio.3000943.ref018","doi-asserted-by":"crossref","first-page":"1610","DOI":"10.1126\/science.280.5369.1610","article-title":"Axonal swellings and degeneration in mice lacking the major proteolipid of myelin","volume":"280","author":"I Griffiths","year":"1998","journal-title":"Science"},{"issue":"1","key":"pbio.3000943.ref019","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1083\/jcb.200312012","article-title":"Oligodendroglial modulation of fast axonal transport in a mouse model of hereditary spastic paraplegia","volume":"166","author":"JM Edgar","year":"2004","journal-title":"J Cell Biol"},{"issue":"2","key":"pbio.3000943.ref020","doi-asserted-by":"crossref","first-page":"107492","DOI":"10.1016\/j.jsb.2020.107492","article-title":"Pathology of myelinated axons in the PLP-deficient mouse model of spastic paraplegia type 2 revealed by volume imaging using focused ion beam-scanning electron microscopy","volume":"210","author":"AM Steyer","year":"2020","journal-title":"J Struct Biol"},{"issue":"1","key":"pbio.3000943.ref021","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1002\/glia.22922","article-title":"Proteolipid protein modulates preservation of peripheral axons and premature death when myelin protein zero is lacking","volume":"64","author":"J Patzig","year":"2016","journal-title":"Glia"},{"issue":"37","key":"pbio.3000943.ref022","doi-asserted-by":"crossref","first-page":"15651","DOI":"10.1073\/pnas.0904764106","article-title":"Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators","volume":"106","author":"H Imamura","year":"2009","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"pbio.3000943.ref023","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1038\/jcbfm.1992.107","article-title":"Ion homeostasis in rat brain in vivo: intra- and extracellular Ca2+ and H+ in the hippocampus during recovery from short-term, transient ischemia","volume":"12","author":"IA Silver","year":"1992","journal-title":"J Cereb Blood Flow Metab"},{"issue":"1\u20132","key":"pbio.3000943.ref024","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/0006-8993(92)90496-V","article-title":"Changes in intracellular pH of mouse hippocampal slices responding to hypoxia and\/or glucose depletion","volume":"572","author":"N Fujiwara","year":"1992","journal-title":"Brain Res"},{"issue":"3\u20134","key":"pbio.3000943.ref025","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.ceca.2004.02.013","article-title":"Calcium and pH homeostasis in neurons during hypoxia and ischemia","volume":"36","author":"H Yao","year":"2004","journal-title":"Cell Calcium"},{"issue":"4","key":"pbio.3000943.ref026","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1038\/jcbfm.1992.83","article-title":"Anoxia-induced changes in extracellular K+ and pH in mammalian central white matter","volume":"12","author":"BR Ransom","year":"1992","journal-title":"J Cereb Blood Flow Metab"},{"issue":"6","key":"pbio.3000943.ref027","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1016\/j.conb.2013.09.008","article-title":"The role of myelin and oligodendrocytes in axonal energy metabolism","volume":"23","author":"AS Saab","year":"2013","journal-title":"Curr Opin Neurobiol"},{"issue":"2","key":"pbio.3000943.ref028","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1134\/S0006297914020084","article-title":"Study on ATP concentration changes in cytosol of individual cultured neurons during glutamate-induced deregulation of calcium homeostasis","volume":"79","author":"AM Surin","year":"2014","journal-title":"Biochemistry (Mosc)"},{"issue":"8","key":"pbio.3000943.ref029","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1002\/jnr.24361","article-title":"FRET\u2010based imaging of intracellular ATP in organotypic brain slices","volume":"97","author":"R Lerchundi","year":"2019","journal-title":"J Neurosci Res"},{"issue":"4","key":"pbio.3000943.ref030","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1016\/j.neuroscience.2009.10.028","article-title":"Molecular disruptions of the panglial syncytium block potassium siphoning and axonal saltatory conduction: pertinence to neuromyelitis optica and other demyelinating diseases of the central nervous system","volume":"168","author":"JE Rash","year":"2010","journal-title":"Neuroscience"},{"issue":"2","key":"pbio.3000943.ref031","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1002\/1097-4547(20010115)63:2<151::AID-JNR1007>3.0.CO;2-Y","article-title":"Myelin proteolipid proteins promote the interaction of oligodendrocytes and axons","volume":"63","author":"DA Yool","year":"2001","journal-title":"J Neurosci Res"},{"issue":"11","key":"pbio.3000943.ref032","doi-asserted-by":"crossref","first-page":"1832","DOI":"10.1002\/glia.22561","article-title":"Uncoupling of neuroinflammation from axonal degeneration in mice lacking the myelin protein tetraspanin-2","volume":"61","author":"P de Monasterio-Schrader","year":"2013","journal-title":"Glia"},{"issue":"3","key":"pbio.3000943.ref033","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1002\/glia.20370","article-title":"Subtle myelin defects in PLP-null mice","volume":"54","author":"J Rosenbluth","year":"2006","journal-title":"Glia"},{"issue":"Pt A","key":"pbio.3000943.ref034","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.brainres.2016.02.027","article-title":"Electron microscopy of myelin: Structure preservation by high-pressure freezing","volume":"1641","author":"W M\u00f6bius","year":"2016","journal-title":"Brain Res"},{"issue":"2","key":"pbio.3000943.ref035","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.celrep.2016.12.053","article-title":"Antagonistic functions of MBP and CNP establish cytosolic channels in CNS myelin","volume":"18","author":"N Snaidero","year":"2017","journal-title":"Cell Rep"},{"issue":"4","key":"pbio.3000943.ref036","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1002\/glia.23549","article-title":"Maintenance of high proteolipid protein level in adult central nervous system myelin is required to preserve the integrity of myelin and axons","volume":"67","author":"KA L\u00fcders","year":"2019","journal-title":"Glia"},{"key":"pbio.3000943.ref037","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1113\/jphysiol.2003.042416","article-title":"Glycogen regulation and functional role in mouse white matter","volume":"549","author":"Pt 2","year":"2003","journal-title":"J Physiol"},{"issue":"18","key":"pbio.3000943.ref038","doi-asserted-by":"crossref","first-page":"6804","DOI":"10.1523\/JNEUROSCI.20-18-06804.2000","article-title":"Astrocytic glycogen influences axon function and survival during glucose deprivation in central white matter","volume":"20","author":"R Wender","year":"2000","journal-title":"J Neurosci"},{"issue":"5","key":"pbio.3000943.ref039","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1002\/jnr.10081","article-title":"Metabolic substrates other than glucose support axon function in central white matter","volume":"66","author":"AM Brown","year":"2001","journal-title":"J Neurosci Res"},{"issue":"1","key":"pbio.3000943.ref040","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.neuroimage.2010.11.022","article-title":"Visualization of cytoplasmic diffusion within living myelin sheaths of CNS white matter axons using microinjection of the fluorescent dye Lucifer Yellow","volume":"56","author":"AA Velumian","year":"2011","journal-title":"Neuroimage"},{"key":"pbio.3000943.ref041","doi-asserted-by":"crossref","first-page":"467","DOI":"10.3389\/fnins.2018.00467","article-title":"The axon-myelin unit in development and degenerative disease","volume":"12","author":"RM Stassart","year":"2018","journal-title":"Front Neurosci"},{"issue":"1","key":"pbio.3000943.ref042","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s00401-019-01985-2","article-title":"Ketogenic diet ameliorates axonal defects and promotes myelination in Pelizaeus-Merzbacher disease","volume":"138","author":"SK Stumpf","year":"2019","journal-title":"Acta Neuropathol"},{"issue":"1","key":"pbio.3000943.ref043","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/0006-8993(91)91154-S","article-title":"Compound action potential of nerve recorded by suction electrode: a theoretical and experimental analysis","volume":"546","author":"PK Stys","year":"1991","journal-title":"Brain Res"},{"issue":"2","key":"pbio.3000943.ref044","doi-asserted-by":"crossref","first-page":"e57712","DOI":"10.1371\/journal.pone.0057712","article-title":"A genetically encoded FRET lactate sensor and its use to detect the Warburg effect in single cancer cells","volume":"8","author":"A San Mart\u00edn","year":"2013","journal-title":"PLoS ONE"},{"key":"pbio.3000943.ref045","doi-asserted-by":"crossref","first-page":"26","DOI":"10.3389\/fnene.2010.00026","article-title":"High resolution measurement of the glycolytic rate","volume":"2","author":"CX Bittner","year":"2010","journal-title":"Front Neuroenergetics"},{"issue":"7","key":"pbio.3000943.ref046","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"J Schindelin","year":"2012","journal-title":"Nat Methods"},{"issue":"1","key":"pbio.3000943.ref047","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1002\/cne.903380110","article-title":"Morphology of astrocytes and oligodendrocytes during development in the intact rat optic nerve","volume":"338","author":"AM Butt","year":"1993","journal-title":"J Comp Neurol"},{"issue":"23","key":"pbio.3000943.ref048","doi-asserted-by":"crossref","first-page":"9781","DOI":"10.1523\/JNEUROSCI.5582-12.2013","article-title":"Integrin-linked kinase regulates process extension in oligodendrocytes via control of actin cytoskeletal dynamics","volume":"33","author":"RW O\u2019Meara","year":"2013","journal-title":"J Neurosci"},{"issue":"8","key":"pbio.3000943.ref049","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1007\/BF01184071","article-title":"Three-dimensional morphology of astrocytes and oligodendrocytes in the intact mouse optic nerve","volume":"23","author":"AM Butt","year":"1994","journal-title":"J Neurocytol"},{"issue":"1","key":"pbio.3000943.ref050","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/0165-5728(94)90125-2","article-title":"Morphological changes in oligodendrocytes in the intact mouse optic nerve following intravitreal injection of tumour necrosis factor","volume":"51","author":"AM Butt","year":"1994","journal-title":"J Neuroimmunol"},{"key":"pbio.3000943.ref051","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/978-1-4939-9072-6_3","article-title":"Myelin: methods for purification and proteome analysis","volume":"1936","author":"MS Erwig","year":"2019","journal-title":"Methods Mol Biol"},{"issue":"29","key":"pbio.3000943.ref052","doi-asserted-by":"crossref","first-page":"7717","DOI":"10.1523\/JNEUROSCI.1254-07.2007","article-title":"Proteolipid protein is required for transport of sirtuin 2 into CNS myelin","volume":"27","author":"HB Werner","year":"2007","journal-title":"J Neurosci"},{"issue":"1","key":"pbio.3000943.ref053","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1186\/s40478-017-0497-6","article-title":"Blood-brain barrier hyperpermeability precedes demyelination in the cuprizone model","volume":"5","author":"SA Berghoff","year":"2017","journal-title":"Acta Neuropathol Commun"},{"issue":"24","key":"pbio.3000943.ref054","doi-asserted-by":"crossref","first-page":"7920","DOI":"10.1523\/JNEUROSCI.16-24-07920.1996","article-title":"Monoclonal antibody O10 defines a conformationally sensitive cell-surface epitope of proteolipid protein (PLP): evidence that PLP misfolding underlies dysmyelination in mutant mice","volume":"16","author":"M Jung","year":"1996","journal-title":"J Neurosci"},{"key":"pbio.3000943.ref055","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1007\/978-1-4939-9072-6_20","article-title":"Transmission electron microscopy of oligodendrocytes and myelin","volume":"1936","author":"M-T Weil","year":"2019","journal-title":"Methods Mol Biol"},{"key":"pbio.3000943.ref056","doi-asserted-by":"crossref","first-page":"e43888","DOI":"10.7554\/eLife.43888","article-title":"Anillin facilitates septin assembly to prevent pathological outfoldings of central nervous system myelin","volume":"8","author":"MS Erwig","year":"2019","journal-title":"Elife"},{"key":"pbio.3000943.ref057","volume-title":"SBEM Protocol v7_01_10. NCMIR methods for 3D EM: a new protocol for preparation of biological specimens for serial blockface scanning electron microscopy","author":"TJ Deerinck","year":"2020"},{"issue":"1","key":"pbio.3000943.ref058","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1006\/jsbi.1996.0013","article-title":"Computer visualization of three-dimensional image data using IMOD","volume":"116","author":"JR Kremer","year":"1996","journal-title":"J Struct Biol"},{"issue":"1","key":"pbio.3000943.ref059","doi-asserted-by":"crossref","first-page":"e1002340","DOI":"10.1371\/journal.pbio.1002340","article-title":"Microscopy Image Browser: a platform for segmentation and analysis of multidimensional datasets","volume":"14","author":"I Belevich","year":"2016","journal-title":"PLoS Biol"}],"updated-by":[{"DOI":"10.1371\/journal.pbio.3000943","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2020,11,30]],"date-time":"2020-11-30T00:00:00Z","timestamp":1606694400000}}],"container-title":["PLOS Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pbio.3000943","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,30]],"date-time":"2020-11-30T14:33:36Z","timestamp":1606746816000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pbio.3000943"}},"subtitle":[],"editor":[{"given":"Kelly R.","family":"Monk","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,11,16]]},"references-count":59,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,11,16]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pbio.3000943","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.pbio.3000943","asserted-by":"object"}]},"ISSN":["1545-7885"],"issn-type":[{"value":"1545-7885","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,16]]}}}