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However, much less is known about the signaling pathways downstream of neurexin\u2019s largely invariant intracellular domain (ICD). <jats:italic>Caenorhabditis elegans<\/jats:italic> contains a single neurexin gene that we have previously shown is required for presynaptic assembly and stabilization. To gain insight into the signaling pathways mediating neurexin\u2019s presynaptic functions, we employed a proximity ligation method, endogenously tagging neurexin\u2019s intracellular domain with the promiscuous biotin ligase TurboID, allowing us to isolate adjacent biotinylated proteins by streptavidin pull-down and mass spectrometry. We compared our experimental strain to a control strain in which neurexin, endogenously tagged with TurboID, was dispersed from presynaptic active zones by the deletion of its C-terminal PDZ-binding motif. Selection of this control strain, which differs from the experimental strain only in its synaptic localization, was critical to identifying interactions specifically occurring at synapses. Using this approach, we identified both known and novel intracellular interactors of neurexin, including active zone scaffolds, actin-binding proteins (including almost every member of the Arp2\/3 complex), signaling molecules, and mediators of RNA trafficking, protein synthesis and degradation, among others. Characterization of mutants for candidate neurexin interactors revealed that they recapitulate aspects of the <jats:italic>nrx-1(-)<\/jats:italic> mutant phenotype, suggesting they may be involved in neurexin signaling. Finally, to investigate a possible role for neurexin in local actin assembly, we endogenously tagged its intracellular domain with actin depolymerizing and sequestering peptides (DeActs) and found that this led to defects in active zone assembly. Together, these results suggest neurexin\u2019s intracellular domain may be involved in presynaptic actin-assembly, and furthermore highlight a novel approach to achieving high specificity for <jats:italic>in vivo<\/jats:italic> proteomics experiments.<\/jats:p>","DOI":"10.1371\/journal.pbio.3002466","type":"journal-article","created":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T18:20:56Z","timestamp":1705947656000},"page":"e3002466","update-policy":"https:\/\/doi.org\/10.1371\/journal.pbio.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Characterization of the intracellular neurexin interactome by in vivo proximity ligation suggests its involvement in presynaptic actin assembly"],"prefix":"10.1371","volume":"22","author":[{"given":"Marcos","family":"Schaan 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