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Using conventional approaches, we found that genes\n                    <jats:italic>Rv3377c<\/jats:italic>\n                    and\n                    <jats:italic>Rv3378c<\/jats:italic>\n                    , but not\n                    <jats:italic>Rv3376<\/jats:italic>\n                    , were necessary for 1-TbAd biosynthesis. Here, we introduce\n                    <jats:underline>l<\/jats:underline>\n                    inear\n                    <jats:underline>m<\/jats:underline>\n                    odels for\n                    <jats:underline>m<\/jats:underline>\n                    ass\n                    <jats:underline>s<\/jats:underline>\n                    pectrometry\n                    <jats:italic>(limms)<\/jats:italic>\n                    software as a next-generation lipidomics tool to study the essential functions of lipid biosynthetic enzymes on a whole-cell basis. Using\n                    <jats:italic>limms<\/jats:italic>\n                    , whole-cell lipid profiles deepened the phenotypic landscape of comparative mass spectrometry experiments and identified a large family of approximately 100 terpene nucleoside metabolites downstream of\n                    <jats:italic>Rv3378c<\/jats:italic>\n                    . We validated the identity of previously unknown adenine-, adenosine-, and lipid-modified tuberculosinol-containing molecules using synthetic chemistry and collisional mass spectrometry, including comprehensive profiling of bacterial lipids that fragment to adenine. We tracked terpene nucleoside genotypes and lipid phenotypes among\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    complex (MTC) species that did or did not evolve to productively infect either human or nonhuman mammals. Although 1-TbAd biosynthesis genes were thought to be restricted to the MTC, we identified the locus in unexpected species outside the MTC. Sequence analysis of the locus showed nucleotide usage characteristic of plasmids from plant-associated bacteria, clarifying the origin and timing of horizontal gene transfer to a pre-MTC progenitor. The data demonstrated correlation between high level terpene nucleoside biosynthesis and mycobacterial competence for human infection, and 2 mechanisms of 1-TbAd biosynthesis loss. Overall, the selective gain and evolutionary retention of tuberculosinyl metabolites in modern species that cause human TB suggest a role in human TB disease, and the newly discovered molecules represent candidate disease-specific biomarkers.\n                  <\/jats:p>","DOI":"10.1371\/journal.pbio.3002813","type":"journal-article","created":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T13:38:38Z","timestamp":1727703518000},"page":"e3002813","update-policy":"https:\/\/doi.org\/10.1371\/journal.pbio.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Mycobacteria that cause tuberculosis have retained ancestrally acquired genes for the biosynthesis of chemically diverse terpene nucleosides"],"prefix":"10.1371","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5762-6755","authenticated-orcid":true,"given":"Jacob 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