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While intercellular DIP::Dpr interactions have been well characterized, DIPs and Dprs are often co-expressed within the same cells, raising the question as to whether they also interact in\n                    <jats:italic>cis<\/jats:italic>\n                    . We show, in cultured cells and\n                    <jats:italic>in vivo<\/jats:italic>\n                    , that DIP-\u03b1 and DIP-\u03b4 can interact in\n                    <jats:italic>cis<\/jats:italic>\n                    with their ligands, Dpr6\/10 and Dpr12, respectively. When co-expressed in\n                    <jats:italic>cis<\/jats:italic>\n                    with their cognate partners, these Dprs regulate the extent of\n                    <jats:italic>trans<\/jats:italic>\n                    binding, presumably through competitive\n                    <jats:italic>cis<\/jats:italic>\n                    interactions. We demonstrate the neurodevelopmental effects of\n                    <jats:italic>cis<\/jats:italic>\n                    inhibition in fly motor neurons and in the mushroom body. We further show that a long disordered region of DIP-\u03b1 at the C-terminus is required for\n                    <jats:italic>cis<\/jats:italic>\n                    but not\n                    <jats:italic>trans<\/jats:italic>\n                    interactions, likely because it alleviates geometric constraints on\n                    <jats:italic>cis<\/jats:italic>\n                    binding. Thus, the balance between\n                    <jats:italic>cis<\/jats:italic>\n                    and\n                    <jats:italic>trans<\/jats:italic>\n                    interactions plays a role in controlling neural development.\n                  <\/jats:p>","DOI":"10.1371\/journal.pbio.3003030","type":"journal-article","created":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T13:29:59Z","timestamp":1741008599000},"page":"e3003030","update-policy":"https:\/\/doi.org\/10.1371\/journal.pbio.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Members of the DIP and Dpr adhesion protein families use cis inhibition to shape neural development in Drosophila"],"prefix":"10.1371","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8129-4033","authenticated-orcid":true,"given":"Nicholas C.","family":"Morano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davys 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