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While bacteria use various mechanisms to compromise host membranes, the specific processes and factors involved are often unknown.\n                    <jats:italic>Shigella flexneri<\/jats:italic>\n                    , a major human pathogen, accesses the cytosol relying on the Type Three Secretion System (T3SS) and secreted effectors. Using in-cell correlative light and electron microscopy, we tracked the sequential steps of\n                    <jats:italic>Shigella<\/jats:italic>\n                    host cell entry. Moreover, we captured the T3SS, which projects a needle from the bacterial surface, in the process of puncturing holes in the vacuolar membrane. This initial puncture ensures disruption of the vacuole. 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