{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T12:34:40Z","timestamp":1780490080830,"version":"3.54.1"},"update-to":[{"DOI":"10.1371\/journal.pbio.3003364","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T00:00:00Z","timestamp":1759449600000}}],"reference-count":76,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T00:00:00Z","timestamp":1757980800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010463","name":"Institut Curie","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100010463","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008017","name":"Institut Gustave-Roussy","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008017","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CNRS"},{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00c3\u00a9 et de la Recherche M\u00c3\u00a9dicale","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"name":"ANR","award":["ANR-10-EQPX-03"],"award-info":[{"award-number":["ANR-10-EQPX-03"]}]},{"name":"ANR","award":["ANR-10-INBS-09-08"],"award-info":[{"award-number":["ANR-10-INBS-09-08"]}]},{"DOI":"10.13039\/100018694","name":"HORIZON EUROPE Marie Sklodowska-Curie Actions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018694","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FRM"},{"DOI":"10.13039\/100018694","name":"HORIZON EUROPE Marie Sklodowska-Curie Actions","doi-asserted-by":"publisher","award":["PRESTIGE-2017-3-0017"],"award-info":[{"award-number":["PRESTIGE-2017-3-0017"]}],"id":[{"id":"10.13039\/100018694","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosbiology.org"],"crossmark-restriction":false},"short-container-title":["PLoS Biol"],"abstract":"<jats:p>Although metabolic benefits of glycolysis have been extensively described in tumor cells, the extra-metabolic functions linked to this energetic pathway in tumor growth and cell proliferation have not been clearly established yet. Recently, some key glycolytic enzymes, such as glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase 2, were reported to regulate mRNA translation. Translational control of gene expression is considered as a critical effector in cancer biology, representing a highly promising area of research. Here, we report that Hexokinase 2 (HK2), a glucose kinase that catalyzes the first step of glycolysis at the outer mitochondrial membrane (OMM), is an RNA-binding protein (RBP) that regulates mRNA translation in melanoma cell lines. Polysome profiling experiments followed by RNA sequencing indicate that the translational regulation exerted by HK2 is partly independent of the metabolic status or the glycolytic pathway. We found that HK2 specifically regulates translation of the mRNA encoding SOX10, a transcription factor implicated in the regulation of tumor initiation, maintenance, and progression in melanoma. RNA-protein interaction assays, including CrossLinking ImmunoPrecipitation (CLIP), indicate that HK2 is an RBP whose interaction with RNA is independent of its enzymatic activity, its ability to bind glucose or its association with the OMM. HK2 directly interacts with the 5\u2032 untranslated region (5\u2032UTR) of the <jats:italic>SOX10<\/jats:italic> mRNA through a stem-loop RNA secondary structure. Using RNA-protein proximity ligation assays and a fluorescence-based ribosome-bound mRNA mapping method, we found that high glucose conditions, which promote the release of HK2 from the OMM, induce an increase in HK2-<jats:italic>SOX10<\/jats:italic> mRNA interaction and <jats:italic>SOX10<\/jats:italic> mRNA translation in the cytoplasm. We further showed that HK2-dependent <jats:italic>SOX10<\/jats:italic> mRNA translation is involved in melanoma cell proliferation and colony formation. Collectively, our data highlight a nonmetabolic function of HK2 acting as an RBP and translation regulator.<\/jats:p>","DOI":"10.1371\/journal.pbio.3003364","type":"journal-article","created":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T17:37:41Z","timestamp":1758044261000},"page":"e3003364","update-policy":"https:\/\/doi.org\/10.1371\/journal.pbio.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Hexokinase 2 is an RNA-binding protein that regulates mRNA translation independently of glycolysis and induces melanoma cell proliferation"],"prefix":"10.1371","volume":"23","author":[{"given":"Ana Luisa","family":"Dian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucilla","family":"Fabbri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Moya-Plana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppina","family":"Claps","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana C.","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line M.","family":"Labb\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Quidville","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain","family":"Martineau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doroth\u00e9e","family":"Baille","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laetitia","family":"Besse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9dric","family":"Messaoudi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9verine","family":"Roy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Raynal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain","family":"Baulande","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wael M.","family":"Rabeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Robert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1452-7164","authenticated-orcid":true,"given":"St\u00e9phan","family":"Vagner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,9,16]]},"reference":[{"issue":"3215","key":"pbio.3003364.ref001","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1126\/science.124.3215.269","article-title":"On respiratory impairment in cancer cells","volume":"124","author":"O Warburg","year":"1956","journal-title":"Science"},{"issue":"2","key":"pbio.3003364.ref002","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1038\/s42255-020-0172-2","article-title":"We need to talk about the Warburg effect","volume":"2","author":"RJ DeBerardinis","year":"2020","journal-title":"Nat Metab"},{"key":"pbio.3003364.ref003","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1146\/annurev-cellbio-092910-154237","article-title":"Aerobic glycolysis: meeting the metabolic requirements of cell proliferation","volume":"27","author":"SY Lunt","year":"2011","journal-title":"Annu Rev Cell Dev Biol"},{"issue":"5930","key":"pbio.3003364.ref004","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1126\/science.1160809","article-title":"Understanding the Warburg effect: the metabolic requirements of cell proliferation","volume":"324","author":"MG Vander Heiden","year":"2009","journal-title":"Science"},{"issue":"12","key":"pbio.3003364.ref005","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1016\/j.tem.2015.09.012","article-title":"Metabolic enzymes enjoying new partnerships as RNA-binding proteins","volume":"26","author":"A Castello","year":"2015","journal-title":"Trends Endocrinol Metab"},{"issue":"4","key":"pbio.3003364.ref006","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1016\/j.molcel.2016.06.029","article-title":"Comprehensive identification of RNA-binding domains in human cells","volume":"63","author":"A Castello","year":"2016","journal-title":"Mol Cell"},{"issue":"6","key":"pbio.3003364.ref007","doi-asserted-by":"crossref","first-page":"2755","DOI":"10.1074\/jbc.270.6.2755","article-title":"Glyceraldehyde-3-phosphate dehydrogenase selectively binds AU-rich RNA in the NAD(+)-binding region (Rossmann fold)","volume":"270","author":"E Nagy","year":"1995","journal-title":"J Biol Chem"},{"key":"pbio.3003364.ref008","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S1874-6047(08)60210-3","article-title":"2 Evolutionary and structural relationships among dehydrogenases","author":"MG Rossman","year":"1975","journal-title":"The Enzymes"},{"issue":"2","key":"pbio.3003364.ref009","doi-asserted-by":"crossref","first-page":"3344","DOI":"10.18632\/oncotarget.13763","article-title":"Hexokinase 2 enhances the metastatic potential of tongue squamous cell carcinoma via the SOD2-H2O2 pathway","volume":"8","author":"W Wang","year":"2017","journal-title":"Oncotarget"},{"issue":"2","key":"pbio.3003364.ref010","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1084\/jem.20101470","article-title":"Hexokinase 2 is a key mediator of aerobic glycolysis and promotes tumor growth in human glioblastoma multiforme","volume":"208","author":"A Wolf","year":"2011","journal-title":"J Exp Med"},{"issue":"2","key":"pbio.3003364.ref011","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.ccr.2013.06.014","article-title":"Hexokinase 2 is required for tumor initiation and maintenance and its systemic deletion is therapeutic in mouse models of cancer","volume":"24","author":"KC Patra","year":"2013","journal-title":"Cancer Cell"},{"key":"pbio.3003364.ref012","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1186\/s12863-016-0459-1","article-title":"Effect of lentivirus-mediated shRNA inactivation of HK1, HK2, and HK3 genes in colorectal cancer and melanoma cells","volume":"17","author":"AV Kudryavtseva","year":"2016","journal-title":"BMC Genet"},{"issue":"5","key":"pbio.3003364.ref013","doi-asserted-by":"crossref","first-page":"428","DOI":"10.4161\/cbt.23786","article-title":"Excess glucose induces hypoxia-inducible factor-1\u03b1 in pancreatic cancer cells and stimulates glucose metabolism and cell migration","volume":"14","author":"Z Liu","year":"2013","journal-title":"Cancer Biol Ther"},{"issue":"1","key":"pbio.3003364.ref014","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1038\/s41467-022-28440-3","article-title":"A non-catalytic scaffolding activity of hexokinase 2 contributes to EMT and metastasis","volume":"13","author":"CS Blaha","year":"2022","journal-title":"Nat Commun"},{"key":"pbio.3003364.ref015","doi-asserted-by":"crossref","DOI":"10.1016\/j.cell.2018.11.004","article-title":"The human RNA-binding proteome and its dynamics during translational arrest","volume":"176","author":"J Trendel","year":"2019","journal-title":"Cell"},{"issue":"2","key":"pbio.3003364.ref016","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1038\/s41587-018-0001-2","article-title":"Comprehensive identification of RNA-protein interactions in any organism using orthogonal organic phase separation (OOPS)","volume":"37","author":"RML Queiroz","year":"2019","journal-title":"Nat Biotechnol"},{"issue":"1","key":"pbio.3003364.ref017","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1038\/s41467-019-08942-3","article-title":"Purification of cross-linked RNA-protein complexes by phenol-toluol extraction","volume":"10","author":"EC Urdaneta","year":"2019","journal-title":"Nat Commun"},{"issue":"9","key":"pbio.3003364.ref018","doi-asserted-by":"crossref","first-page":"4725","DOI":"10.1093\/nar\/gkaa256","article-title":"Plasticity of nuclear and cytoplasmic stress responses of RNA-binding proteins","volume":"48","author":"M Backlund","year":"2020","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"pbio.3003364.ref019","doi-asserted-by":"crossref","DOI":"10.1016\/j.cell.2022.12.004","article-title":"Glucose dissociates DDX21 dimers to regulate mRNA splicing and tissue differentiation","volume":"186","author":"W Miao","year":"2023","journal-title":"Cell"},{"issue":"6","key":"pbio.3003364.ref020","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1016\/j.cell.2013.05.016","article-title":"Posttranscriptional control of T cell effector function by aerobic glycolysis","volume":"153","author":"C-H Chang","year":"2013","journal-title":"Cell"},{"issue":"6","key":"pbio.3003364.ref021","doi-asserted-by":"crossref","DOI":"10.1016\/j.cell.2017.05.022","article-title":"The mammalian ribo-interactome reveals ribosome functional diversity and heterogeneity","volume":"169","author":"D Simsek","year":"2017","journal-title":"Cell"},{"issue":"49","key":"pbio.3003364.ref022","doi-asserted-by":"crossref","first-page":"42626","DOI":"10.1074\/jbc.M111.282046","article-title":"Comparative metabolic flux profiling of melanoma cell lines: beyond the Warburg effect","volume":"286","author":"DA Scott","year":"2011","journal-title":"J Biol Chem"},{"issue":"1","key":"pbio.3003364.ref023","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.bbrc.2010.06.057","article-title":"Targeting glutamine metabolism sensitizes melanoma cells to TRAIL-induced death","volume":"398","author":"J-Z Qin","year":"2010","journal-title":"Biochem Biophys Res Commun"},{"issue":"12","key":"pbio.3003364.ref024","doi-asserted-by":"crossref","first-page":"6461","DOI":"10.1093\/nar\/gkad440","article-title":"Pyruvate Kinase M (PKM) binds ribosomes in a poly-ADP ribosylation dependent manner to induce translational stalling","volume":"51","author":"NS Kejiou","year":"2023","journal-title":"Nucleic Acids Res"},{"key":"pbio.3003364.ref025","doi-asserted-by":"crossref","first-page":"2935","DOI":"10.1038\/ncomms3935","article-title":"Tumour-associated mutant p53 drives the Warburg effect","volume":"4","author":"C Zhang","year":"2013","journal-title":"Nat Commun"},{"issue":"3","key":"pbio.3003364.ref026","doi-asserted-by":"crossref","DOI":"10.1016\/j.cmet.2018.08.012","article-title":"Glycolytic stimulation is not a requirement for M2 macrophage differentiation","volume":"28","author":"F Wang","year":"2018","journal-title":"Cell Metab"},{"issue":"19","key":"pbio.3003364.ref027","doi-asserted-by":"crossref","first-page":"8788","DOI":"10.1073\/pnas.1003428107","article-title":"Mitochondrial metabolism and ROS generation are essential for Kras-mediated tumorigenicity","volume":"107","author":"F Weinberg","year":"2010","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"pbio.3003364.ref028","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1038\/s41467-017-02354-x","article-title":"ERK-mediated phosphorylation regulates SOX10 sumoylation and targets expression in mutant BRAF melanoma","volume":"9","author":"S Han","year":"2018","journal-title":"Nat Commun"},{"issue":"8","key":"pbio.3003364.ref029","doi-asserted-by":"crossref","first-page":"2212","DOI":"10.1038\/jid.2014.128","article-title":"SOX10 promotes melanoma cell invasion by regulating melanoma inhibitory activity","volume":"134","author":"SA Graf","year":"2014","journal-title":"J Invest Dermatol"},{"issue":"1","key":"pbio.3003364.ref030","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0190834","article-title":"Identification and functional analysis of SOX10 phosphorylation sites in melanoma","volume":"13","author":"JC Cronin","year":"2018","journal-title":"PLoS One"},{"issue":"4","key":"pbio.3003364.ref031","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1158\/2159-8290.CD-13-0440","article-title":"Response of BRAF-mutant melanoma to BRAF inhibition is mediated by a network of transcriptional regulators of glycolysis","volume":"4","author":"TJ Parmenter","year":"2014","journal-title":"Cancer Discov"},{"issue":"3","key":"pbio.3003364.ref032","doi-asserted-by":"crossref","DOI":"10.26508\/lsa.201800088","article-title":"Silica-based solid-phase extraction of cross-linked nucleic acid-bound proteins","volume":"1","author":"C Asencio","year":"2018","journal-title":"Life Sci Alliance"},{"issue":"3","key":"pbio.3003364.ref033","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.molcel.2018.01.005","article-title":"Advances in CLIP technologies for studies of protein-RNA interactions","volume":"69","author":"FCY Lee","year":"2018","journal-title":"Mol Cell"},{"issue":"1","key":"pbio.3003364.ref034","doi-asserted-by":"crossref","DOI":"10.1038\/s43586-021-00018-1","article-title":"CLIP and complementary methods","volume":"1","author":"M Hafner","year":"2021","journal-title":"Nat Rev Methods Primers"},{"issue":"3","key":"pbio.3003364.ref035","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1016\/j.cell.2016.05.076","article-title":"Hexokinase is an innate immune receptor for the detection of bacterial peptidoglycan","volume":"166","author":"AJ Wolf","year":"2016","journal-title":"Cell"},{"issue":"9","key":"pbio.3003364.ref036","article-title":"Aerobic glycolysis promotes tumor immune evasion by hexokinase2-mediated phosphorylation of I\u03baB\u03b1","volume":"34","author":"D Guo","year":"2022","journal-title":"Cell Metab"},{"issue":"1","key":"pbio.3003364.ref037","doi-asserted-by":"crossref","DOI":"10.1042\/BSR20171666","article-title":"The catalytic inactivation of the N-half of human hexokinase 2 and structural and biochemical characterization of its mitochondrial conformation","volume":"38","author":"MH Nawaz","year":"2018","journal-title":"Biosci Rep"},{"issue":"1","key":"pbio.3003364.ref038","doi-asserted-by":"crossref","DOI":"10.1016\/j.molcel.2019.04.018","article-title":"R-DeeP: proteome-wide and quantitative identification of RNA-dependent proteins by density gradient ultracentrifugation","volume":"75","author":"M Caudron-Herger","year":"2019","journal-title":"Mol Cell"},{"key":"pbio.3003364.ref039","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkab393","article-title":"catRAPID omics v2.0: going deeper and wider in the prediction of protein-RNA interactions","volume":"49","author":"A Armaos","year":"2021","journal-title":"Nucleic Acids Res"},{"issue":"11","key":"pbio.3003364.ref040","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1261\/rna.058248.116","article-title":"A proximity-dependent assay for specific RNA-protein interactions in intact cells","volume":"22","author":"W Zhang","year":"2016","journal-title":"RNA"},{"issue":"8016","key":"pbio.3003364.ref041","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate structure prediction of biomolecular interactions with AlphaFold 3","volume":"630","author":"J Abramson","year":"2024","journal-title":"Nature"},{"issue":"34","key":"pbio.3003364.ref042","doi-asserted-by":"crossref","first-page":"4683","DOI":"10.1038\/sj.onc.1209595","article-title":"Mitochondrial hexokinases, novel mediators of the antiapoptotic effects of growth factors and Akt","volume":"25","author":"RB Robey","year":"2006","journal-title":"Oncogene"},{"issue":"6652","key":"pbio.3003364.ref043","doi-asserted-by":"crossref","DOI":"10.1126\/science.add3067","article-title":"Spatially resolved single-cell translatomics at molecular resolution","volume":"380","author":"H Zeng","year":"2023","journal-title":"Science"},{"issue":"1","key":"pbio.3003364.ref044","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1038\/s41467-022-28801-y","article-title":"Targeting SOX10-deficient cells to reduce the dormant-invasive phenotype state in melanoma","volume":"13","author":"C Capparelli","year":"2022","journal-title":"Nat Commun"},{"issue":"8","key":"pbio.3003364.ref045","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1038\/s41556-020-0547-3","article-title":"Robust gene expression programs underlie recurrent cell states and phenotype switching in melanoma","volume":"22","author":"J Wouters","year":"2020","journal-title":"Nat Cell Biol"},{"issue":"7494","key":"pbio.3003364.ref046","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1038\/nature13121","article-title":"Reversible and adaptive resistance to BRAF(V600E) inhibition in melanoma","volume":"508","author":"C Sun","year":"2014","journal-title":"Nature"},{"issue":"8","key":"pbio.3003364.ref047","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1038\/ncb2535","article-title":"Sox10 promotes the formation and maintenance of giant congenital naevi and melanoma","volume":"14","author":"O Shakhova","year":"2012","journal-title":"Nat Cell Biol"},{"issue":"8","key":"pbio.3003364.ref048","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1038\/s41419-023-06009-2","article-title":"Hexokinase 2 confers radio-resistance in hepatocellular carcinoma by promoting autophagy-dependent degradation of AIMP2","volume":"14","author":"Y Zheng","year":"2023","journal-title":"Cell Death Dis"},{"key":"pbio.3003364.ref049","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1042\/bj20031465","article-title":"In self-defence: hexokinase promotes voltage-dependent anion channel closure and prevents mitochondria-mediated apoptotic cell death","volume":"377","author":"H Azoulay-Zohar","year":"2004","journal-title":"Biochem J"},{"issue":"11","key":"pbio.3003364.ref050","doi-asserted-by":"crossref","first-page":"1446","DOI":"10.1261\/rna.079210.122","article-title":"The mutual interaction of glycolytic enzymes and RNA in post-transcriptional regulation","volume":"28","author":"M Wegener","year":"2022","journal-title":"RNA"},{"issue":"3","key":"pbio.3003364.ref051","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.bbrc.2011.01.034","article-title":"Binding of glyceraldehyde-3-phosphate dehydrogenase to the cis-acting element of structure-anchored repression in ccn2 mRNA","volume":"405","author":"S Kondo","year":"2011","journal-title":"Biochem Biophys Res Commun"},{"issue":"9","key":"pbio.3003364.ref052","doi-asserted-by":"crossref","first-page":"3762","DOI":"10.1158\/0008-5472.CAN-04-3954","article-title":"Glyceraldehyde-3-phosphate dehydrogenase binds to the AU-Rich 3\u2019 untranslated region of colony-stimulating factor-1 (CSF-1) messenger RNA in human ovarian cancer cells: possible role in CSF-1 posttranscriptional regulation and tumor phenotype","volume":"65","author":"N Bonaf\u00e9","year":"2005","journal-title":"Cancer Res"},{"issue":"8","key":"pbio.3003364.ref053","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1158\/1541-7786.MCR-07-2170","article-title":"The multifunctional protein glyceraldehyde-3-phosphate dehydrogenase is both regulated and controls colony-stimulating factor-1 messenger RNA stability in ovarian cancer","volume":"6","author":"Y Zhou","year":"2008","journal-title":"Mol Cancer Res"},{"issue":"2","key":"pbio.3003364.ref054","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.abb.2012.09.004","article-title":"Glyceraldehyde-3-phosphate dehydrogenase regulates cyclooxygenase-2 expression by targeting mRNA stability","volume":"528","author":"Y Ikeda","year":"2012","journal-title":"Arch Biochem Biophys"},{"key":"pbio.3003364.ref055","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1007\/978-1-4939-7634-8_15","article-title":"High-resolution, high-throughput analysis of Hfq-binding sites using UV crosslinking and analysis of cDNA (CRAC).","author":"B Sy","year":"2018","journal-title":"Bacterial regulatory RNA: methods and protocols"},{"issue":"11","key":"pbio.3003364.ref056","doi-asserted-by":"crossref","first-page":"1324","DOI":"10.1134\/S0006297917110104","article-title":"Factors beyond enolase 2 and mitochondrial Lysyl-tRNA synthetase precursor are required for tRNA import into yeast mitochondria","volume":"82","author":"MV Baleva","year":"2017","journal-title":"Biochemistry (Mosc)"},{"issue":"5","key":"pbio.3003364.ref057","doi-asserted-by":"crossref","first-page":"9354","DOI":"10.3390\/ijms16059354","article-title":"A moonlighting human protein is involved in mitochondrial import of tRNA","volume":"16","author":"M Baleva","year":"2015","journal-title":"Int J Mol Sci"},{"issue":"12","key":"pbio.3003364.ref058","doi-asserted-by":"crossref","first-page":"1609","DOI":"10.1101\/gad.385706","article-title":"A glycolytic enzyme, enolase, is recruited as a cofactor of tRNA targeting toward mitochondria in Saccharomyces cerevisiae","volume":"20","author":"N Entelis","year":"2006","journal-title":"Genes Dev"},{"issue":"5093","key":"pbio.3003364.ref059","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1126\/science.8420004","article-title":"Sequence-specific binding of transfer RNA by glyceraldehyde-3-phosphate dehydrogenase","volume":"259","author":"R Singh","year":"1993","journal-title":"Science"},{"issue":"1","key":"pbio.3003364.ref060","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1038\/s41392-020-00444-9","article-title":"The regulation of protein translation and its implications for cancer","volume":"6","author":"P Song","year":"2021","journal-title":"Signal Transduct Target Ther"},{"issue":"9","key":"pbio.3003364.ref061","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1038\/s41568-021-00380-y","article-title":"The plasticity of mRNA translation during cancer progression and therapy resistance","volume":"21","author":"L Fabbri","year":"2021","journal-title":"Nat Rev Cancer"},{"issue":"9","key":"pbio.3003364.ref062","doi-asserted-by":"crossref","first-page":"2574","DOI":"10.1016\/j.drudis.2022.05.017","article-title":"The development of small-molecule inhibitors targeting hexokinase 2","volume":"27","author":"W Shan","year":"2022","journal-title":"Drug Discov Today"},{"issue":"2","key":"pbio.3003364.ref063","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1158\/1541-7786.MCR-23-0290","article-title":"SOX10 loss sensitizes melanoma cells to cytokine-mediated inflammatory cell death","volume":"22","author":"SR Rosenbaum","year":"2024","journal-title":"Mol Cancer Res"},{"issue":"12","key":"pbio.3003364.ref064","doi-asserted-by":"crossref","first-page":"2726","DOI":"10.1002\/ijc.23449","article-title":"Aerobic glycolysis in cancers: implications for the usability of oxygen-responsive genes and fluorodeoxyglucose-PET as markers of tissue hypoxia","volume":"122","author":"M Busk","year":"2008","journal-title":"Int J Cancer"},{"issue":"14","key":"pbio.3003364.ref065","doi-asserted-by":"crossref","DOI":"10.1016\/j.molcel.2022.05.019","article-title":"Riboregulation of Enolase 1 activity controls glycolysis and embryonic stem cell differentiation","volume":"82","author":"I Huppertz","year":"2022","journal-title":"Mol Cell"},{"issue":"1","key":"pbio.3003364.ref066","doi-asserted-by":"crossref","first-page":"5713","DOI":"10.1038\/s41467-019-13360-6","article-title":"An epitranscriptomic mechanism underlies selective mRNA translation remodelling in melanoma persister cells","volume":"10","author":"S Shen","year":"2019","journal-title":"Nat Commun"},{"issue":"7516","key":"pbio.3003364.ref067","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1038\/nature13572","article-title":"eIF4F is a nexus of resistance to anti-BRAF and anti-MEK cancer therapies","volume":"513","author":"L Boussemart","year":"2014","journal-title":"Nature"},{"key":"pbio.3003364.ref068","author":"N Servant","year":"2023","journal-title":"Bioinfo-pf-curie\/RNA-seq: v4.0.0"},{"key":"pbio.3003364.ref069","doi-asserted-by":"crossref","first-page":"11194","DOI":"10.1038\/ncomms11194","article-title":"Genome-wide assessment of differential translations with ribosome profiling data","volume":"7","author":"Z Xiao","year":"2016","journal-title":"Nat Commun"},{"issue":"12","key":"pbio.3003364.ref070","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"MI Love","year":"2014","journal-title":"Genome Biol"},{"key":"pbio.3003364.ref071","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/978-0-387-98141-3_10","article-title":"Reducing duplication","author":"H Wickham","year":"2009","journal-title":"ggplot2"},{"key":"pbio.3003364.ref072","doi-asserted-by":"crossref","first-page":"100071","DOI":"10.1074\/jbc.RA120.015293","article-title":"Linker residues regulate the activity and stability of hexokinase 2, a promising anticancer target","volume":"296","author":"JC Ferreira","year":"2021","journal-title":"J Biol Chem"},{"issue":"3","key":"pbio.3003364.ref073","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0092444","article-title":"ColonyArea: an ImageJ plugin to automatically quantify colony formation in clonogenic assays","volume":"9","author":"C Guzm\u00e1n","year":"2014","journal-title":"PLoS One"},{"issue":"1","key":"pbio.3003364.ref074","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1038\/s41592-020-01018-x","article-title":"Cellpose: a generalist algorithm for cellular segmentation","volume":"18","author":"C Stringer","year":"2021","journal-title":"Nat Methods"},{"issue":"14","key":"pbio.3003364.ref075","doi-asserted-by":"crossref","first-page":"1840","DOI":"10.1093\/bioinformatics\/btt276","article-title":"TANGO: a generic tool for high-throughput 3D image analysis for studying nuclear organization","volume":"29","author":"J Ollion","year":"2013","journal-title":"Bioinformatics"},{"key":"pbio.3003364.ref076","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1111\/j.1365-2818.2006.01706.x","article-title":"A guided tour into subcellular colocalization analysis in light microscopy","volume":"224","author":"S Bolte","year":"2006","journal-title":"J Microsc"}],"updated-by":[{"DOI":"10.1371\/journal.pbio.3003364","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T00:00:00Z","timestamp":1759449600000}}],"container-title":["PLOS Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pbio.3003364","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T17:42:58Z","timestamp":1759513378000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pbio.3003364"}},"subtitle":[],"editor":[{"given":"Elena","family":"Rainero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,9,16]]},"references-count":76,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,9,16]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pbio.3003364","relation":{},"ISSN":["1545-7885"],"issn-type":[{"value":"1545-7885","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,16]]}}}