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However, it remains elusive why organisms have evolved such an astonishing diversity of isoforms. Here, we show that fitness and immunity properties have driven the modern evolution of <jats:italic><jats:italic>Dscam1<\/jats:italic><\/jats:italic> isoform diversity. We assess multiple aspects of fly fitness in deletion mutants harboring exon 4, 6, or 9 clusters, respectively, reducing ectodomain isoform diversity stepwise from 18,612 to 396. All fitness-related traits generally improved as the potential number of isoforms increased; however, the magnitude of the changes varied remarkably in a variable cluster-specific manner. Correlation analysis revealed that fitness-related traits were much more sensitive to reductions in Dscam1 diversity compared to canonical neuronal self\/non-self discrimination. We conclude that the role of Dscam1 isoforms in canonical neuronal self-avoidance and self\/non-self discrimination is mediated by a small fraction of all isoforms (&lt;1\/10), whereas a separate role essential for other developmental contexts and resistances, likely in fitness and immunity, requires almost full isoform diversity. Thus, fitness and immunity properties, rather than canonical neuronal functions, are the dominant drivers during the modern diversification of the Dscam1 isoform. Our findings suggest that Dscam1 diversity is closely linked to adaptation and species diversification in arthropods.<\/jats:p>","DOI":"10.1371\/journal.pbio.3003383","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T17:38:22Z","timestamp":1757698702000},"page":"e3003383","update-policy":"https:\/\/doi.org\/10.1371\/journal.pbio.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["The modern expansion of Dscam1 isoform diversity in Drosophila is linked to fitness and 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